TRACHTOVÁ, Štěpánka, Ondřel KAMAN, Alena ŠPANOVÁ, Pavel VEVERKA, Emil POLLERT and Bohuslav RITTICH. Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation. (Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation.). Journal of Separation Science. Weinheim: Wiley-VCH, 2011, vol. 34, No 21, p. 3077-3082. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201100442. |
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@article{951843, author = {Trachtová, Štěpánka and Kaman, Ondřel and Španová, Alena and Veverka, Pavel and Pollert, Emil and Rittich, Bohuslav}, article_location = {Weinheim}, article_number = {21}, doi = {http://dx.doi.org/10.1002/jssc.201100442}, keywords = {DNA isolation; lanthanum manganese perovskite; nanoparticles; PCR; silica shell}, language = {eng}, issn = {1615-9306}, journal = {Journal of Separation Science}, title = {Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation.}, volume = {34}, year = {2011} }
TY - JOUR ID - 951843 AU - Trachtová, Štěpánka - Kaman, Ondřel - Španová, Alena - Veverka, Pavel - Pollert, Emil - Rittich, Bohuslav PY - 2011 TI - Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation. JF - Journal of Separation Science VL - 34 IS - 21 SP - 3077-3082 EP - 3077-3082 PB - Wiley-VCH SN - 16159306 KW - DNA isolation KW - lanthanum manganese perovskite KW - nanoparticles KW - PCR KW - silica shell N2 - Magnetic La0.75Sr0.25MnO3 nanoparticles possessing an approximately 20 nm thick silica shell (LSMO(0.25)@SiO2) were characterised and tested for the isolation of PCR-ready bacterial DNA. The results presented here show that thenanoparticles do not interfere in PCR. DNA was reversibly adsorbed on their silica shell from the aqueous phase system (16 % PEG 6000 – 2 M NaCl). The method proposed was used for DNA isolation from complex food samples (dairy products and probiotic food supplements). The isolated DNA was compatible with PCR. The main advantages of the nanoparticles tested for routine use were their high colloidal stability allowing a more precise dosage and therefore high reproducibility of DNA isolation. ER -
TRACHTOVÁ, Štěpánka, Ondřel KAMAN, Alena ŠPANOVÁ, Pavel VEVERKA, Emil POLLERT and Bohuslav RITTICH. Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation. (Silica coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation.). \textit{Journal of Separation Science}. Weinheim: Wiley-VCH, 2011, vol.~34, No~21, p.~3077-3082. ISSN~1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201100442.
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