Neuroinflammatory reaction of peripheral glial cells in two
different models of neuropathic pain

p 2011

Neuroinflammatory reaction of peripheral glial cells in two different models of neuropathic pain

DUBOVÝ, Petr; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ; Václav BRÁZDA; Lucie STREJČKOVÁ et al.

Základní údaje

Originální název

Neuroinflammatory reaction of peripheral glial cells in two different models of neuropathic pain

Autoři

DUBOVÝ, Petr; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ; Václav BRÁZDA a Lucie STREJČKOVÁ

Vydání

Morphology 2011, Praha, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Vyžádané přednášky

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Schwann cells; satellite glial cells; cytokines

Změněno: 17. 2. 2012 08:08, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

Many experimental studies have documented a correlation of pro-inflammatory cytokine elevations with induction and maintenance of neuropathic pain. In our experiments, Wistar rats operated on unilateral chronic constriction injury of the sciatic nerve and sham-operated rats were allowed to survive for 1, 3, 7 and 14 days. Withdrawal thresholds for mechanical allodynia and thermal hyperalgesia were measured in the ipsi- and contralateral hind and fore paws. Expression of TNFa and IL-6 mRNAs was investigated by in situ hybridization and quantified by means of qRT-PCR in L4-L5 (homonymous) and C7-C8 (heteronymous) dorsal root ganglia (DRG). Bilateral up-regulation of TNFa and IL-6 mRNAs was found in the neurons and satellite glial cells of DRG from all investigated segments. TNFa mRNA was peaked in L4-L5 DRG at one day while IL-6 mRNA three days from CCI. Elevation of TNFa and IL-6 mRNAs in C7-C8 DRG was of lower magnitude when compared with L4-L5 DRG. An increased level of cytokine mRNAs was measured and in situ hybridization staining was found in satellite glial cells of DRG from sham-operated animals. The changes of TNFa and IL-6 mRNAs in DRG did not correlate with mechanical allodynia and thermal hyperalgesia.

Návaznosti

MSM0021622404, záměr

Název: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Citovat

DUBOVÝ, Petr; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ; Václav BRÁZDA a Lucie STREJČKOVÁ. Neuroinflammatory reaction of peripheral glial cells in two different models of neuropathic pain. In Morphology 2011, Praha. 2011.

@misc{951863,
   author = {Dubový, Petr and Hradilová Svíženská, Ivana and Klusáková, Ilona and Brázda, Václav and Strejčková, Lucie},
   booktitle = {Morphology 2011, Praha},
   keywords = {Schwann cells; satellite glial cells; cytokines},
   language = {eng},
   title = {Neuroinflammatory reaction of peripheral glial cells in two different models of neuropathic pain},
   year = {2011}
}

TY  - SLIDE
ID  - 951863
AU  - Dubový, Petr - Hradilová Svíženská, Ivana - Klusáková, Ilona - Brázda, Václav - Strejčková, Lucie
PY  - 2011
TI  - Neuroinflammatory reaction of peripheral glial cells in two different models of neuropathic pain
KW  - Schwann cells
KW  - satellite glial cells
KW  - cytokines
N2  - Many experimental studies have documented a correlation of pro-inflammatory cytokine elevations with induction and maintenance of neuropathic pain. In our experiments, Wistar rats operated on unilateral chronic constriction injury of the sciatic nerve and sham-operated rats were allowed to survive for 1, 3, 7 and 14 days. Withdrawal thresholds for mechanical allodynia and thermal hyperalgesia were measured in the ipsi- and contralateral hind and fore paws. Expression of TNFa and IL-6 mRNAs was investigated by in situ hybridization and quantified by means of qRT-PCR in L4-L5 (homonymous) and C7-C8 (heteronymous) dorsal root ganglia (DRG). Bilateral up-regulation of TNFa and IL-6 mRNAs was found in the neurons and satellite glial cells of DRG from all investigated segments. TNFa mRNA was peaked in L4-L5 DRG at one day while IL-6 mRNA three days from CCI. Elevation of TNFa and IL-6 mRNAs in C7-C8 DRG was of lower magnitude when compared with L4-L5 DRG. An increased level of cytokine mRNAs was measured and in situ hybridization staining was found in satellite glial cells of DRG from sham-operated animals. The changes of TNFa and IL-6 mRNAs in DRG did not correlate with mechanical allodynia and thermal hyperalgesia.
ER  -

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