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@article{952522, author = {Gelbíčová, Tereza and Karpíšková, Renata}, article_number = {1}, keywords = {virulence; listeria; antimicrobial resistance; restriction fragment length polymorphism; pulsed-field gel electrophoresis (PFGE)}, language = {eng}, issn = {1212-1800}, journal = {Czech Journal of Food Sciences}, note = {Opraven obor publikace}, title = {Outdoor environment as a source of Listeria monocytogenes in food chain}, volume = {30}, year = {2012} }
TY - JOUR ID - 952522 AU - Gelbíčová, Tereza - Karpíšková, Renata PY - 2012 TI - Outdoor environment as a source of Listeria monocytogenes in food chain JF - Czech Journal of Food Sciences VL - 30 IS - 1 SP - 83-88 EP - 83-88 SN - 12121800 N1 - Opraven obor publikace KW - virulence KW - listeria KW - antimicrobial resistance KW - restriction fragment length polymorphism KW - pulsed-field gel electrophoresis (PFGE) N2 - The aims of this study were to monitor the presence of Listeria monocytogenes in environmental sources and to evaluate the phenotypic and molecular characteristics of the isolates recovered. L. monocytogenes was isolated in 12 (11.2%) of the 107 samples from the wild, farm environment, and vegetation. Most isolates (83.3%) were of serotype 1/2a and the remainder (2) were of serotype 4b. All 12 isolates were susceptible to the whole range of antimicrobials tested. These12 strains were carriers of the virulence genes prfA, hlyA, actA, plcA, plcB, inlA, inlB, inlC, and inlJ. The detection of the inlA gene in 4 (33.3%) of 12 strains using the PCR-RFLP suggests the potential of some of these strains to penetrate into epithelial cells of the intestinal barrier. Macrorestriction analysis also confirmed clonal identity of some environmental isolates with food and human isolates. These results indicate that the external environment is a source of potentially pathogenic strains of L. monocytogenes. ER -
GELBÍČOVÁ, Tereza a Renata KARPÍŠKOVÁ. Outdoor environment as a source of Listeria monocytogenes in food chain. \textit{Czech Journal of Food Sciences}. 2012, roč.~30, č.~1, s.~83-88. ISSN~1212-1800.
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