Influence of the lipopolysaccharide structure of Salmonella
enterica serovar Enteritidis on interactions with pig
neutrophils

J 2011

Influence of the lipopolysaccharide structure of Salmonella enterica serovar Enteritidis on interactions with pig neutrophils

MATIAŠOVIC, Ján; Hana ŠTEPÁNOVÁ; Jiří VOLF; Lukáš KUBALA; Petra OVESNÁ et al.

Základní údaje

Originální název

Influence of the lipopolysaccharide structure of Salmonella enterica serovar Enteritidis on interactions with pig neutrophils

Autoři

MATIAŠOVIC, Ján; Hana ŠTEPÁNOVÁ; Jiří VOLF; Lukáš KUBALA; Petra OVESNÁ ; Ivan RYCHLÍK a Martin FALDYNA

Vydání

Veterinary Microbiology, 2011, 0378-1135

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.327

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/11:00053445

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.vetmic.2011.01.007

UT WoS

000290696500022

Klíčová slova anglicky

Salmonella; Pig; Pathogen-associated molecular patterns; Lipopolysaccharide; Neutrophil

Příznaky

Mezinárodní význam

Změněno: 11. 4. 2012 15:54, Mgr. Michal Petr

Anotace

V originále

The key process for immune response development is the recognition of bacteria by the immune system of the host based on the sensing of pathogen-associated molecular patterns (PAMP). One of the most important PAMP is the lipopolysaccharide (LPS) molecule, a complex molecule present in the outer membrane of Gram negative bacteria. In this study we were interested in how different parts of the LPS of Salmonella enterica serovar Enteritidis are recognized by porcine neutrophils. To this aim, we constructed S. Enteritidis mutants with rfaL and rfaC genes disabled in the attachment of the O-antigen and in the synthesis of the inner oligosaccharide core of LPS, respectively.We found that in the absence of serum, both the rfa mutants associated with neutrophils and stimulated them for reactive oxygen species (ROS) production significantly more than the wild-type strain. Addition of polymyxin B, which neutralized lipid A, the endotoxic moiety of LPS, effectively decreased the association of the wild-type strain and the rfaC mutant with neutrophils, but not the rfaL mutant. This indicates that the oligosaccharide core newly exposed on the surface in the rfaL mutant, protected from interaction in the wild-type strain by the O-antigen but completely absent in the rfaC mutant, may represent a new ligand for porcine neutrophils that cannot be neutralized by polymyxin B.

Citovat

MATIAŠOVIC, Ján; Hana ŠTEPÁNOVÁ; Jiří VOLF; Lukáš KUBALA; Petra OVESNÁ; Ivan RYCHLÍK a Martin FALDYNA. Influence of the lipopolysaccharide structure of Salmonella enterica serovar Enteritidis on interactions with pig neutrophils. Veterinary Microbiology. 2011, roč. 150, 1-2, s. 167-172. ISSN 0378-1135. Dostupné z: https://doi.org/10.1016/j.vetmic.2011.01.007.

@article{953114,
   author = {Matiašovic, Ján and Štepánová, Hana and Volf, Jiří and Kubala, Lukáš and Ovesná, Petra and Rychlík, Ivan and Faldyna, Martin},
   article_number = {1-2},
   doi = {https://doi.org/10.1016/j.vetmic.2011.01.007},
   keywords = {Salmonella; Pig; Pathogen-associated molecular patterns; Lipopolysaccharide; Neutrophil},
   language = {eng},
   issn = {0378-1135},
   journal = {Veterinary Microbiology},
   title = {Influence of the lipopolysaccharide structure of Salmonella enterica serovar Enteritidis on interactions with pig neutrophils},
   volume = {150},
   year = {2011}
}

TY  - JOUR
ID  - 953114
AU  - Matiašovic, Ján - Štepánová, Hana - Volf, Jiří - Kubala, Lukáš - Ovesná, Petra - Rychlík, Ivan - Faldyna, Martin
PY  - 2011
TI  - Influence of the lipopolysaccharide structure of Salmonella enterica serovar Enteritidis on interactions with pig neutrophils
JF  - Veterinary Microbiology
VL  - 150
IS  - 1-2
SP  - 167-172
EP  - 167-172
SN  - 03781135
KW  - Salmonella
KW  - Pig
KW  - Pathogen-associated molecular patterns
KW  - Lipopolysaccharide
KW  - Neutrophil
N2  - The key process for immune response development is the recognition of bacteria by the immune system of the host based on the sensing of pathogen-associated molecular patterns (PAMP). One of the most important PAMP is the lipopolysaccharide (LPS) molecule, a complex molecule present in the outer membrane of Gram negative bacteria. In this study we were interested in how different parts of the LPS of Salmonella enterica serovar Enteritidis are recognized by porcine neutrophils. To this aim, we constructed S. Enteritidis mutants with rfaL and rfaC genes disabled in the attachment of the O-antigen and in the synthesis of the inner oligosaccharide core of LPS, respectively.We found that in the absence of serum, both the rfa mutants associated with neutrophils and stimulated them for reactive oxygen species (ROS) production significantly more than the wild-type strain. Addition of polymyxin B, which neutralized lipid A, the endotoxic moiety of LPS, effectively decreased the association of the wild-type strain and the rfaC mutant with neutrophils, but not the rfaL mutant. This indicates that the oligosaccharide core newly exposed on the surface in the rfaL mutant, protected from interaction in the wild-type strain by the O-antigen but completely absent in the rfaC mutant, may represent a new ligand for porcine neutrophils that cannot be neutralized by polymyxin B.
ER  -

MATIAŠOVIC, Ján; Hana ŠTEPÁNOVÁ; Jiří VOLF; Lukáš KUBALA; Petra OVESNÁ; Ivan RYCHLÍK a Martin FALDYNA. Influence of the lipopolysaccharide structure of Salmonella enterica serovar Enteritidis on interactions with pig neutrophils. \textit{Veterinary Microbiology}. 2011, roč.~150, 1-2, s.~167-172. ISSN~0378-1135. Dostupné z: https://doi.org/10.1016/j.vetmic.2011.01.007.

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