Microfluidic robotic device coupled with electrochemical sensor
field for handling of paramagnetic micro-particles as a tool
for determination of plant mRNA.

J 2011

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.

HUSKA, Dalibor, Vojtěch ADAM, Petr BABULA, Libuše TRNKOVÁ, Jaromír HUBÁLEK et. al.

Základní údaje

Originální název

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.

Autoři

HUSKA, Dalibor (203 Česká republika), Vojtěch ADAM (203 Česká republika), Petr BABULA (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, domácí), Jaromír HUBÁLEK (203 Česká republika), Josef ZEHNÁLEK (203 Česká republika), Ladislav HAVEL (203 Česká republika) a René KIZEK (203 Česká republika, garant)

Vydání

Microchim. Acta, Austria, 2011, 0026-3672

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.033

Kód RIV

RIV/00216224:14310/11:00053553

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s00604-011-0545-z

UT WoS

000290522800025

Klíčová slova anglicky

Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 6. 2020 13:45, Mgr. Marie Šípková, DiS.

Anotace

V originále

The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

HUSKA, Dalibor, Vojtěch ADAM, Petr BABULA, Libuše TRNKOVÁ, Jaromír HUBÁLEK, Josef ZEHNÁLEK, Ladislav HAVEL a René KIZEK. Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA. Microchim. Acta. Austria, 2011, roč. 173, 1-2, s. 189-197. ISSN 0026-3672. Dostupné z: https://dx.doi.org/10.1007/s00604-011-0545-z.

@article{954022,
   author = {Huska, Dalibor and Adam, Vojtěch and Babula, Petr and Trnková, Libuše and Hubálek, Jaromír and Zehnálek, Josef and Havel, Ladislav and Kizek, René},
   article_location = {Austria},
   article_number = {1-2},
   doi = {http://dx.doi.org/10.1007/s00604-011-0545-z},
   keywords = {Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles},
   language = {eng},
   issn = {0026-3672},
   journal = {Microchim. Acta},
   title = {Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.},
   url = {https://link.springer.com/article/10.1007/s00604-011-0545-z},
   volume = {173},
   year = {2011}
}

TY  - JOUR
ID  - 954022
AU  - Huska, Dalibor - Adam, Vojtěch - Babula, Petr - Trnková, Libuše - Hubálek, Jaromír - Zehnálek, Josef - Havel, Ladislav - Kizek, René
PY  - 2011
TI  - Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.
JF  - Microchim. Acta
VL  - 173
IS  - 1-2
SP  - 189-197
EP  - 189-197
SN  - 00263672
KW  - Electrochemical detection
KW  - Microfluidics
KW  - Environmental stress
KW  - Cadmium ions
KW  - Paramagnetic particles
UR  - https://link.springer.com/article/10.1007/s00604-011-0545-z
L2  - https://link.springer.com/article/10.1007/s00604-011-0545-z
N2  - The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.
ER  -

HUSKA, Dalibor, Vojtěch ADAM, Petr BABULA, Libuše TRNKOVÁ, Jaromír HUBÁLEK, Josef ZEHNÁLEK, Ladislav HAVEL a René KIZEK. Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA. \textit{Microchim. Acta}. Austria, 2011, roč.~173, 1-2, s.~189-197. ISSN~0026-3672. Dostupné z: https://dx.doi.org/10.1007/s00604-011-0545-z.

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