Analytical methods for metallothionein detection

J 2011

Analytical methods for metallothionein detection

RYVOLOVÁ, Markéta, Soňa KRIZKOVA, Vojtěch ADAM, M. BEKLOVA, Libuše TRNKOVÁ et. al.

Základní údaje

Originální název

Analytical methods for metallothionein detection

Autoři

RYVOLOVÁ, Markéta (203 Česká republika), Soňa KRIZKOVA (203 Česká republika), Vojtěch ADAM (203 Česká republika), M. BEKLOVA (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, domácí), Jaromír HUBÁLEK (203 Česká republika) a René KIZEK (203 Česká republika, garant)

Vydání

Curr. Anal. Chem. 2011, 1573-4110

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.000

Kód RIV

RIV/00216224:14310/11:00053554

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000293130200010

Klíčová slova anglicky

Metallothionein; Electrochemistry; Immunochemistry; Gel electrophoresis; Capillary electrophoresis; Chromatography; Spectroscopy; Mass spectrometry; Saturation assays; Cancer; Environmental analysis

Štítky

AKR, rivok

Změněno: 12. 3. 2012 11:01, Ing. Andrea Mikešková

Anotace

V originále

Metallothioneins (MTs) are a group of low molecular mass, cysteine-rich proteins with a variety of functions including involvement in metal homeostasis, free radical scavenging, protection against heavy metal damage, and metabolic regulation via Zn donation. The overexpression of MTs in proliferating cells is turning the attention to the study of MTs as novel promising marker of tumor diseases. Besides, levels of MTs in invertebrates and aquatic vertebrates well correlate with heavy metal pollution of an environment and, thus, serve as bio-environmental marker. It is not surprising that these proteins are of great interest not only for biochemists, molecular biologists clinical chemists, but also for environmental chemists and ecologists. Detection and quantification of MTs, which is needed in all above mentioned areas, is challenging due to the high cysteine content and relatively low molecular mass. This review is giving an overview of the analytical methods used for determination of MTs comprising a broad range of immunochemical, electrochemical as well as spectrometric methods, which have been optimized and even hyphenated with different separation methods to detect MTs. More attention is paid to the structural analysis of these proteins as well as the employment of analytical instruments for environmental, biochemical and clinical purposes.

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

RYVOLOVÁ, Markéta, Soňa KRIZKOVA, Vojtěch ADAM, M. BEKLOVA, Libuše TRNKOVÁ, Jaromír HUBÁLEK a René KIZEK. Analytical methods for metallothionein detection. Curr. Anal. Chem. 2011, roč. 7, č. 3, s. 243-261. ISSN 1573-4110.

@article{954024,
   author = {Ryvolová, Markéta and KRIZKOVA, Soňa and Adam, Vojtěch and BEKLOVA, M. and Trnková, Libuše and Hubálek, Jaromír and Kizek, René},
   article_number = {3},
   keywords = {Metallothionein; Electrochemistry; Immunochemistry; Gel electrophoresis; Capillary electrophoresis; Chromatography; Spectroscopy; Mass spectrometry; Saturation assays; Cancer; Environmental analysis},
   language = {eng},
   issn = {1573-4110},
   journal = {Curr. Anal. Chem.},
   title = {Analytical methods for metallothionein detection},
   url = {http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=8&SID=V254NakJe2LpBEPl4g1&page=1&doc=1},
   volume = {7},
   year = {2011}
}

TY  - JOUR
ID  - 954024
AU  - Ryvolová, Markéta - KRIZKOVA, Soňa - Adam, Vojtěch - BEKLOVA, M. - Trnková, Libuše - Hubálek, Jaromír - Kizek, René
PY  - 2011
TI  - Analytical methods for metallothionein detection
JF  - Curr. Anal. Chem.
VL  - 7
IS  - 3
SP  - 243-261
EP  - 243-261
SN  - 15734110
KW  - Metallothionein
KW  - Electrochemistry
KW  - Immunochemistry
KW  - Gel electrophoresis
KW  - Capillary electrophoresis
KW  - Chromatography
KW  - Spectroscopy
KW  - Mass spectrometry
KW  - Saturation assays
KW  - Cancer
KW  - Environmental analysis
UR  - http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=8&SID=V254NakJe2LpBEPl4g1&page=1&doc=1
N2  - Metallothioneins (MTs) are a group of low molecular mass, cysteine-rich proteins with a variety of functions including involvement in metal homeostasis, free radical scavenging, protection against heavy metal damage, and metabolic regulation via Zn donation. The overexpression of MTs in proliferating cells is turning the attention to the study of MTs as novel promising marker of tumor diseases. Besides, levels of MTs in invertebrates and aquatic vertebrates well correlate with heavy metal pollution of an environment and, thus, serve as bio-environmental marker. It is not surprising that these proteins are of great interest not only for biochemists, molecular biologists clinical chemists, but also for environmental chemists and ecologists. Detection and quantification of MTs, which is needed in all above mentioned areas, is challenging due to the high cysteine content and relatively low molecular mass. This review is giving an overview of the analytical methods used for determination of MTs comprising a broad range of immunochemical, electrochemical as well as spectrometric methods, which have been optimized and even hyphenated with different separation methods to detect MTs. More attention is paid to the structural analysis of these proteins as well as the employment of analytical instruments for environmental, biochemical and clinical purposes.
ER  -

RYVOLOVÁ, Markéta, Soňa KRIZKOVA, Vojtěch ADAM, M. BEKLOVA, Libuše TRNKOVÁ, Jaromír HUBÁLEK a René KIZEK. Analytical methods for metallothionein detection. \textit{Curr. Anal. Chem.}. 2011, roč.~7, č.~3, s.~243-261. ISSN~1573-4110.

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