Immobilization of metallothionein to carbon paste electrode
surface via anti-MT antibodies and its use for biosensing of
silver.

J 2011

Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver.

TRNKOVÁ, Libuše, Soňa KRIZKOVA, Vojtěch ADAM, Jaromír HUBÁLEK, René KIZEK et. al.

Základní údaje

Originální název

Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver.

Autoři

TRNKOVÁ, Libuše (203 Česká republika, domácí), Soňa KRIZKOVA (203 Česká republika), Vojtěch ADAM (203 Česká republika), Jaromír HUBÁLEK (203 Česká republika) a René KIZEK (203 Česká republika, garant)

Vydání

BIOSENSORS & BIOELECTRONICS, 2011, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.602

Kód RIV

RIV/00216224:14310/11:00053555

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.bios.2010.09.035

UT WoS

000286904400066

Klíčová slova anglicky

Heavy metal; Silver; Metallothionein; Square wave voltammetry; Water; Environmental analysis

Štítky

AKR, rivok

Změněno: 20. 4. 2012 11:16, Ing. Andrea Mikešková

Anotace

V originále

In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on the capturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals - labelled as cys(MT) and W(a) - were observed. The cyst,AT corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 mu g/ml) were optimized to suggest a silver(I) ions biosensor. Biosensor (CPE modified with anti-MT antibody) prepared under the optimized conditions was then used for silver(I) ions detection. The detection limit (3 S/N) for silver(I) ions was estimated as 0.5 nM. The proposed biosensor was tested by detection spiking of silver(I) ions in various water samples (from very pure distilled water to rainwater). Recoveries varied from 74 to 104%. (C) 2010 Elsevier B.V. All rights reserved.

Návaznosti

LC06035, projekt VaV

Název: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

TRNKOVÁ, Libuše, Soňa KRIZKOVA, Vojtěch ADAM, Jaromír HUBÁLEK a René KIZEK. Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver. BIOSENSORS & BIOELECTRONICS. 2011, roč. 26, č. 5, s. 2201-2207. ISSN 0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2010.09.035.

@article{954027,
   author = {Trnková, Libuše and KRIZKOVA, Soňa and Adam, Vojtěch and Hubálek, Jaromír and Kizek, René},
   article_number = {5},
   doi = {http://dx.doi.org/10.1016/j.bios.2010.09.035},
   keywords = {Heavy metal; Silver; Metallothionein; Square wave voltammetry; Water; Environmental analysis},
   language = {eng},
   issn = {0956-5663},
   journal = {BIOSENSORS & BIOELECTRONICS},
   title = {Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver.},
   url = {http://ac.els-cdn.com/S0956566310006494/1-s2.0-S0956566310006494-main.pdf?_tid=45582c491f72810960ab2a3968016b45&acdnat=1332250805_449357aac5e95feac50ebc201b6b9175},
   volume = {26},
   year = {2011}
}

TY  - JOUR
ID  - 954027
AU  - Trnková, Libuše - KRIZKOVA, Soňa - Adam, Vojtěch - Hubálek, Jaromír - Kizek, René
PY  - 2011
TI  - Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver.
JF  - BIOSENSORS & BIOELECTRONICS
VL  - 26
IS  - 5
SP  - 2201-2207
EP  - 2201-2207
SN  - 09565663
KW  - Heavy metal
KW  - Silver
KW  - Metallothionein
KW  - Square wave voltammetry
KW  - Water
KW  - Environmental analysis
UR  - http://ac.els-cdn.com/S0956566310006494/1-s2.0-S0956566310006494-main.pdf?_tid=45582c491f72810960ab2a3968016b45&acdnat=1332250805_449357aac5e95feac50ebc201b6b9175
L2  - http://ac.els-cdn.com/S0956566310006494/1-s2.0-S0956566310006494-main.pdf?_tid=45582c491f72810960ab2a3968016b45&acdnat=1332250805_449357aac5e95feac50ebc201b6b9175
N2  - In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on the capturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals - labelled as cys(MT) and W(a) - were observed. The cyst,AT corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 mu g/ml) were optimized to suggest a silver(I) ions biosensor. Biosensor (CPE modified with anti-MT antibody) prepared under the optimized conditions was then used for silver(I) ions detection. The detection limit (3 S/N) for silver(I) ions was estimated as 0.5 nM. The proposed biosensor was tested by detection spiking of silver(I) ions in various water samples (from very pure distilled water to rainwater). Recoveries varied from 74 to 104%. (C) 2010 Elsevier B.V. All rights reserved.
ER  -

TRNKOVÁ, Libuše, Soňa KRIZKOVA, Vojtěch ADAM, Jaromír HUBÁLEK a René KIZEK. Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver. \textit{BIOSENSORS \&{} BIOELECTRONICS}. 2011, roč.~26, č.~5, s.~2201-2207. ISSN~0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2010.09.035.

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