Electrochemical detection of 8 - hydroxy - 2´-dexyguanosine
isolated from a real sample by use of antibody - modified
paramagnetic particles

D 2011

Electrochemical detection of 8 - hydroxy - 2´-dexyguanosine isolated from a real sample by use of antibody - modified paramagnetic particles

ZÍTKA, Ondřej, Soňa KŘÍŽKOVÁ, Libuše TRNKOVÁ, Aleš HORNA, Jaromír HUBÁLEK et. al.

Základní údaje

Originální název

Electrochemical detection of 8 - hydroxy - 2´-dexyguanosine isolated from a real sample by use of antibody - modified paramagnetic particles

Autoři

ZÍTKA, Ondřej, Soňa KŘÍŽKOVÁ, Libuše TRNKOVÁ, Aleš HORNA, Jaromír HUBÁLEK, Vojtěch ADAM a René KIZEK

Vydání

Pardubice, 11th International Nutrition and Diagnostics conference INDC 2011, od s. 32-32, 122 s. 2011

Nakladatel

Univerzita Pardubice

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10403 Physical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7395-378-2

Klíčová slova anglicky

guanosine;paramagnetic particles;elisa;differential pulse voltammetry

Změněno: 8. 3. 2012 14:52, Mgr. Sylvie Dohnalíková

Anotace

V originále

Guanosine derivatives are important for diagnostic of oxidative DNA damamge. 8 - hydroxy - 2-deoxyguanosine (8-OHdG) is one of most abundant products of DNA oxidation. Its determination in biological fluids, e. g. urine is possible. Nevertheless, owing to its low concentration and matrix interferences (uric acid) use of HPLC without previous extraction is difficult. In this study we tested the isolation of 8-OHdG from a biological matrix by using the paramagnetic particles with antibody - modified surface. We used the sandwich ELISA principles for determination of molecules of 8-OHdG. The primary antibody was bound to a paramgnetic particle, then 8-OHdG was attached during the reaction in the sample. Then the secondary antibody with alkaline phosphatase was attached. Concentration of the analyte was determined from concentration of napthol, which is a product of alkaline phosphatase where napthyl phosphate acts product. Every step during whole process was conducted with a magnetic holder which held the magnetic particles with bound substrate in place in the test tube...

Citovat

ZÍTKA, Ondřej, Soňa KŘÍŽKOVÁ, Libuše TRNKOVÁ, Aleš HORNA, Jaromír HUBÁLEK, Vojtěch ADAM a René KIZEK. Electrochemical detection of 8 - hydroxy - 2´-dexyguanosine isolated from a real sample by use of antibody - modified paramagnetic particles. In J. Blattná, A. Horna, T. Zima, A. Zobel. 11th International Nutrition and Diagnostics conference INDC 2011. Pardubice: Univerzita Pardubice, 2011, s. 32-32, 122 s. ISBN 978-80-7395-378-2.

@inproceedings{954072,
   author = {Zítka, Ondřej and Křížková, Soňa and Trnková, Libuše and Horna, Aleš and Hubálek, Jaromír and Adam, Vojtěch and Kizek, René},
   address = {Pardubice},
   booktitle = {11th International Nutrition and Diagnostics conference INDC 2011},
   editor = {J. Blattná, A. Horna, T. Zima, A. Zobel},
   keywords = {guanosine;paramagnetic particles;elisa;differential pulse voltammetry},
   language = {eng},
   location = {Pardubice},
   isbn = {978-80-7395-378-2},
   pages = {32-32},
   publisher = {Univerzita Pardubice},
   title = {Electrochemical detection of 8 - hydroxy - 2´-dexyguanosine isolated from a real sample by use of antibody - modified paramagnetic particles},
   year = {2011}
}

TY  - JOUR
ID  - 954072
AU  - Zítka, Ondřej - Křížková, Soňa - Trnková, Libuše - Horna, Aleš - Hubálek, Jaromír - Adam, Vojtěch - Kizek, René
PY  - 2011
TI  - Electrochemical detection of 8 - hydroxy - 2´-dexyguanosine isolated from a real sample by use of antibody - modified paramagnetic particles
PB  - Univerzita Pardubice
CY  - Pardubice
SN  - 9788073953782
KW  - guanosine;paramagnetic particles;elisa;differential pulse voltammetry
N2  - Guanosine derivatives are important for diagnostic of oxidative DNA damamge. 8 - hydroxy - 2-deoxyguanosine (8-OHdG) is one of most abundant products of DNA oxidation. Its determination in biological fluids, e. g. urine is possible. Nevertheless, owing to its low concentration and matrix interferences (uric acid) use of HPLC without previous extraction is difficult. In this study we tested the isolation of 8-OHdG from a biological matrix by using the paramagnetic particles with antibody - modified surface. We used the sandwich ELISA principles for determination of molecules of 8-OHdG. The primary antibody was bound to a paramgnetic particle, then 8-OHdG was attached during the reaction in the sample. Then the secondary antibody with alkaline phosphatase was attached. Concentration of the analyte was determined from concentration of napthol, which is a product of alkaline phosphatase where napthyl phosphate acts product. Every step during whole process was conducted with a magnetic holder which held the magnetic particles with bound substrate in place in the test tube...
ER  -

ZÍTKA, Ondřej, Soňa KŘÍŽKOVÁ, Libuše TRNKOVÁ, Aleš HORNA, Jaromír HUBÁLEK, Vojtěch ADAM a René KIZEK. Electrochemical detection of 8 - hydroxy - 2´-dexyguanosine isolated from a real sample by use of antibody - modified paramagnetic particles. In J. Blattná, A. Horna, T. Zima, A. Zobel. \textit{11th International Nutrition and Diagnostics conference INDC 2011}. Pardubice: Univerzita Pardubice, 2011, s.~32-32, 122 s. ISBN~978-80-7395-378-2.

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