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@proceedings{955109,
author = {Lubal, Přemysl and Holubová, Sylvie and Trnková, Libuše},
booktitle = {Biopolymers: Structure and dynamics, A biophysical workshop},
keywords = {oligonucleotides;cytosine;adenine;molecular spectroscopy;equispec;opium},
language = {eng},
isbn = {978-80-263-0050-2},
title = {A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides},
year = {2011}
}
TY - CONF
ID - 955109
AU - Lubal, Přemysl - Holubová, Sylvie - Trnková, Libuše
PY - 2011
TI - A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides
SN - 9788026300502
KW - oligonucleotides;cytosine;adenine;molecular spectroscopy;equispec;opium
N2 - The protonation constants of nucleobases and relevant nucleosides and nucleotides were determined by means of potenciometry and molecular absorption spectroscopy in UV region (I=0.15 mol.l-1 NaCl,t=25C) and the experimental data were evaluated by OPIUM program. In addition, thermodynamic parameters for nucleobase ligands were estimated. Also, some examples of ligand complexation by transition metal ions (e.g. copper(II),zinc(II),kadmium(II)) will be presented and their importance for bioligand speciation will be discussed. The protonation behavior of ODN containing C, i.e.(CCC)n,n=1–3, was followed by means of molecular spectroscopy in pH region 2–8 and variable temperature and ionic strength adjusted by NaCl. The experimental data were treated by means of OPIUM, MCR – ALS and EQUISPEC software and distribution diagram of species as pH fuction were calculated. For comparison, the protonation behavior of ODN with the (CAC)3, A3C3C3 and C3A3C3 sequence was also investigated. The influence of the structural motif and length on protonation constants o studied ODN seems to be very important due to probace stabilization of the secondary structure of the biomacromolecule.
ER -
LUBAL, Přemysl, Sylvie HOLUBOVÁ a Libuše TRNKOVÁ. A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides. In \textit{Biopolymers: Structure and dynamics, A biophysical workshop}. 2011. ISBN~978-80-263-0050-2.
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