Polyethyleneimine coated capillary in nucleotide and short
oligonucleotide analysis

a 2011

Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis

BITTOVÁ, Miroslava, Vendula ROBLOVÁ, Eva MAJEROVÁ a Libuše TRNKOVÁ

Základní údaje

Originální název

Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis

Autoři

BITTOVÁ, Miroslava (203 Česká republika, garant, domácí), Vendula ROBLOVÁ (203 Česká republika, domácí), Eva MAJEROVÁ (203 Česká republika, domácí) a Libuše TRNKOVÁ (203 Česká republika, domácí)

Vydání

8th International Interdisciplinary Meeting on Bioanalysis - CECE 2011, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/11:00053816

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-904959-0-6

Klíčová slova anglicky

capillary electrophoresis polyethyleneimine coating nucleotides oligonucleotides

Štítky

AKR

Změněno: 13. 4. 2012 12:40, prof. RNDr. Libuše Trnková, CSc.

Anotace

V originále

Inner surface modification of fused-silica capillaries in capillary zone electrophoresis with an appropriate chemical reagents allow targeted selection of separation system for analytes of interest. Polyethyleneimine (PEI) is polycationic highly branched polymer with positively charged primary, secondary and tertiary amino groups in the structure that can be used as fused-silica capillary modifier. Its irreversible adsorption on the silanol groups of the fused-silica capillary surface eliminates negative charge and excess of charged amino groups cause reversion of electroosmotic flow. In our work, we successfully applied polyethyleneimine coated capillaries in analysis of nucleotides and synthetic pentamers. We studied the influence of particular component of Tris/boric acid/EDTA buffer (TBE) on the PEI coated capillary surface and the effect of hydroxyethylcellulose (HEC) and/or B-cyclodextrin on the nucleotide separation. Finally, we analyzed DNA restricted fragments from Nicotiana tabacum.

Návaznosti

LC06035, projekt VaV

Název: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu

Citovat

BITTOVÁ, Miroslava, Vendula ROBLOVÁ, Eva MAJEROVÁ a Libuše TRNKOVÁ. Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis. In 8th International Interdisciplinary Meeting on Bioanalysis - CECE 2011. 2011. ISBN 978-80-904959-0-6.

@proceedings{956906,
   author = {Bittová, Miroslava and Roblová, Vendula and Majerová, Eva and Trnková, Libuše},
   booktitle = {8th International Interdisciplinary Meeting on Bioanalysis - CECE 2011},
   keywords = {capillary electrophoresis polyethyleneimine coating nucleotides oligonucleotides},
   language = {eng},
   isbn = {978-80-904959-0-6},
   title = {Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis},
   year = {2011}
}

TY  - CONF
ID  - 956906
AU  - Bittová, Miroslava - Roblová, Vendula - Majerová, Eva - Trnková, Libuše
PY  - 2011
TI  - Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis
SN  - 9788090495906
KW  - capillary electrophoresis polyethyleneimine coating nucleotides oligonucleotides
N2  - Inner surface modification of fused-silica capillaries in capillary zone electrophoresis with an appropriate chemical reagents allow targeted selection of separation system for analytes of interest. Polyethyleneimine (PEI) is polycationic highly branched polymer with positively charged primary, secondary and tertiary amino groups in the structure that can be used as fused-silica capillary modifier. Its irreversible adsorption on the silanol groups of the fused-silica capillary surface eliminates negative charge and excess of charged amino groups cause reversion of electroosmotic flow. In our work, we successfully applied polyethyleneimine coated capillaries in analysis of nucleotides and synthetic pentamers. We studied the influence of particular component of Tris/boric acid/EDTA buffer (TBE) on the PEI coated capillary surface and the effect of hydroxyethylcellulose (HEC) and/or B-cyclodextrin on the nucleotide separation. Finally, we analyzed DNA restricted fragments from Nicotiana tabacum.
ER  -

BITTOVÁ, Miroslava, Vendula ROBLOVÁ, Eva MAJEROVÁ a Libuše TRNKOVÁ. Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis. In \textit{8th International Interdisciplinary Meeting on Bioanalysis - CECE 2011}. 2011. ISBN~978-80-904959-0-6.

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