Contents of Sulforaphane and Total Isothiocyanates,
Antimutagenic Activity, and Inhibition of Clastogenicity in
Pulp Juices from Cruciferous Plants

J 2011

Contents of Sulforaphane and Total Isothiocyanates, Antimutagenic Activity, and Inhibition of Clastogenicity in Pulp Juices from Cruciferous Plants

TOTUŠEK, Jiří; Jan TŘÍSKA; Danuše LEFNEROVÁ; Jan STROHALM; Naděžda VRCHOTOVÁ et al.

Základní údaje

Originální název

Contents of Sulforaphane and Total Isothiocyanates, Antimutagenic Activity, and Inhibition of Clastogenicity in Pulp Juices from Cruciferous Plants

Autoři

TOTUŠEK, Jiří; Jan TŘÍSKA; Danuše LEFNEROVÁ; Jan STROHALM; Naděžda VRCHOTOVÁ; Ondřej ZENDULKA; Jiřina PRŮCHOVÁ; Jana CHALOUPKOVÁ; Pavla NOVOTNÁ a Milan HOUŠKA

Vydání

Czech Journal of Food Sciences, 2011, 1212-1800

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.522

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/11:00056917

Organizační jednotka

Lékařská fakulta

UT WoS

000296874400011

Klíčová slova anglicky

cruciferous vegetables; juice; antimutagenic effects; sulforaphane; isothiocyanates

Příznaky

Mezinárodní význam

Změněno: 11. 4. 2012 15:40, Mgr. Michal Petr

Anotace

V originále

The consumption of Cruciferous vegetables is important for the prevention of cancerous diseases, particularly colorectal cancer. The effects of technological treatments (freezing, pasteurisation, high-pressure treatment) on the content of isothiocyanates, considered to be the active substance, were observed in single-species vegetable juices prepared from cruciferous vegetables (broccoli, cauliflower, Brussels sprouts, white and red cabbage). The contents of sulforaphane and total isothiocyanates were studied relative to the temperature, action period, and time delay after juice pressing. Sulforaphane and total isothiocyanates were determined by HPLC. Sulforaphane content in various parts of fresh broccoli was also assessed. Antimutagenic activity of the juices (frozen, pasteurised, and high-pressure treated) was evaluated using the Ames test and the following mutagens: AFTB1 (aflatoxin B1), IQ (2-amino-3-methyl-3H-imidazo-[ 4,5-f]quinoline), and MNU (2-nitroso-2-methylurea). Clastogenicity inhibition of the mutagens, in response to broccoli juice, as well as of pure sulforaphane, was observed using an in vivo experiment (the micronucleus test). It was shown that in terms of sulforaphane content, it is best to let broccoli juice stand for 60 min after pressing and pH adjustment. Sulforaphane content does not change under heating to 60 deg.C. Its content decreases considerably (compared to fresh juice) with heating to higher temperatures than 60 deg.C. High-pressure treatment preserves mutagenic inhibition to the same degree as juices freezing.

Návaznosti

QF3287, projekt VaV

Název: Funkční potraviny ze zeleniny a ovoce a dalších zemědělských produktů vyrobené za použití vysokotlakého ošetření

Investor: Ministerstvo zemědělství ČR, Funkční potraviny ze zeleniny a ovoce a dalších zemědělských produktů vyrobené za použití vysokotlakého ošetření

Citovat

TOTUŠEK, Jiří; Jan TŘÍSKA; Danuše LEFNEROVÁ; Jan STROHALM; Naděžda VRCHOTOVÁ; Ondřej ZENDULKA; Jiřina PRŮCHOVÁ; Jana CHALOUPKOVÁ; Pavla NOVOTNÁ a Milan HOUŠKA. Contents of Sulforaphane and Total Isothiocyanates, Antimutagenic Activity, and Inhibition of Clastogenicity in Pulp Juices from Cruciferous Plants. Czech Journal of Food Sciences. 2011, roč. 29, č. 5, s. 548–556. ISSN 1212-1800.

@article{957260,
   author = {Totušek, Jiří and Tříska, Jan and Lefnerová, Danuše and Strohalm, Jan and Vrchotová, Naděžda and Zendulka, Ondřej and Průchová, Jiřina and Chaloupková, Jana and Novotná, Pavla and Houška, Milan},
   article_number = {5},
   keywords = {cruciferous vegetables; juice; antimutagenic effects; sulforaphane; isothiocyanates},
   language = {eng},
   issn = {1212-1800},
   journal = {Czech Journal of Food Sciences},
   title = {Contents of Sulforaphane and Total Isothiocyanates, Antimutagenic Activity, and Inhibition of Clastogenicity in Pulp Juices from Cruciferous Plants},
   volume = {29},
   year = {2011}
}

TY  - JOUR
ID  - 957260
AU  - Totušek, Jiří - Tříska, Jan - Lefnerová, Danuše - Strohalm, Jan - Vrchotová, Naděžda - Zendulka, Ondřej - Průchová, Jiřina - Chaloupková, Jana - Novotná, Pavla - Houška, Milan
PY  - 2011
TI  - Contents of Sulforaphane and Total Isothiocyanates, Antimutagenic Activity, and Inhibition of Clastogenicity in Pulp Juices from Cruciferous Plants
JF  - Czech Journal of Food Sciences
VL  - 29
IS  - 5
SP  - 548–556
EP  - 548–556
SN  - 12121800
KW  - cruciferous vegetables
KW  - juice
KW  - antimutagenic effects
KW  - sulforaphane
KW  - isothiocyanates
N2  - The consumption of Cruciferous vegetables is important for the prevention of cancerous diseases, particularly colorectal cancer. The effects of technological treatments (freezing, pasteurisation, high-pressure treatment) on the content of isothiocyanates, considered to be the active substance, were observed in single-species vegetable juices prepared from cruciferous vegetables (broccoli, cauliflower, Brussels sprouts, white and red cabbage). The contents of sulforaphane and total isothiocyanates were studied relative to the temperature, action period, and time delay after juice pressing. Sulforaphane and total isothiocyanates were determined by HPLC. Sulforaphane content in various parts of fresh broccoli was also assessed. Antimutagenic activity of the juices (frozen, pasteurised, and high-pressure treated) was evaluated using the Ames test and the following mutagens: AFTB1 (aflatoxin B1), IQ (2-amino-3-methyl-3H-imidazo-[ 4,5-f]quinoline), and MNU (2-nitroso-2-methylurea). Clastogenicity inhibition of the mutagens, in response to broccoli juice, as well as of pure sulforaphane, was observed using an in vivo experiment (the micronucleus test). It was shown that in terms of sulforaphane content, it is best to let broccoli juice stand for 60 min after pressing and pH adjustment. Sulforaphane content does not change under heating to 60 deg.C. Its content decreases considerably (compared to fresh juice) with heating to higher temperatures than 60 deg.C. High-pressure treatment preserves mutagenic inhibition to the same degree as juices freezing.
ER  -

TOTUŠEK, Jiří; Jan TŘÍSKA; Danuše LEFNEROVÁ; Jan STROHALM; Naděžda VRCHOTOVÁ; Ondřej ZENDULKA; Jiřina PRŮCHOVÁ; Jana CHALOUPKOVÁ; Pavla NOVOTNÁ a Milan HOUŠKA. Contents of Sulforaphane and Total Isothiocyanates, Antimutagenic Activity, and Inhibition of Clastogenicity in Pulp Juices from Cruciferous Plants. \textit{Czech Journal of Food Sciences}. 2011, roč.~29, č.~5, s.~548–556. ISSN~1212-1800.

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