Towards understanding the mechanisms of metastasis in low grade
breast cancer: Discovery of new targets via

a 2011

Towards understanding the mechanisms of metastasis in low grade breast cancer: Discovery of new targets via "omics" techniques

BOUCHAL, Pavel; Monika DVOŘÁKOVÁ; Theodoros ROUMELIOTIS; Zbyněk BORTLÍČEK; Iva STRUHÁROVÁ et al.

Základní údaje

Originální název

Towards understanding the mechanisms of metastasis in low grade breast cancer: Discovery of new targets via "omics" techniques

Autoři

BOUCHAL, Pavel; Monika DVOŘÁKOVÁ; Theodoros ROUMELIOTIS; Zbyněk BORTLÍČEK; Iva STRUHÁROVÁ; Spiros D. GARBIS; Rudolf NENUTIL a Bořivoj VOJTĚŠEK

Vydání

HUPO 2011 10th Annual World Congress, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

breast cancer, proteomics, transcriptomics

Příznaky

Mezinárodní význam

Změněno: 22. 11. 2011 11:47, doc. Mgr. Pavel Bouchal, Ph.D.

Anotace

V originále

Background: Although breast cancer is the most common women cancer in the world, traditional prognostic factors are not sufficient for precise risk-group discrimination. This is well illustrated by the group of low grade breast tumors in which small percentage of tumors form lymph node metastases early. Methods: Our effort has been focused on biomarker discovery in the set of 96 breast cancer primary tumors grouped and randomized according to grade, lymph node and hormonal receptor status. iTRAQ-2DLC-MS/MS and 2-D SDSPAGE proteomics approaches were involved in complex protein analysis of tissue lysates. qRT-PCR assays and immunohistochemistry were used as complementary tools for verification of selected gene products expression. Results: Our results indicate several types of differences between the studied groups of tumors, namely: (i) iTRAQ- 2DLC-MS/MS data were internally validated via ErbB2 protein expression as confirmed by immunohistochemistry. (ii) Biological replicates in the study exhibited positive correlation and clustering of proteomic profiles according to clinicopathological parameters. (iii) Expression of selected cytoskeletal proteins correlated with lymph node status of the tumors. (iv) Expression of AGR family members was strongly affected by estrogene receptor status of the tumors. Conclusion: Our findings provide a solid data for targeted functional studies and validation geared towards more indepth description of metastatic mechanisms in low-grade breast cancer tumors.

Návaznosti

GAP304/10/0868, projekt VaV

Název: Studium molekulárních mechanismů časného metastazování do lymfatických uzlin u karcinomu prsu nízkého stupně malignity pomocí proteomických technik

Investor: Grantová agentura ČR, Studium molekulárních mechanismů časného metastazování do lymfatických uzlin u karcinomu prsu nízkého stupně malignity pomocí proteomických technik

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

MZ0MOU2005, záměr

Název: Funkční diagnostika zhoubných nádorů

Citovat

BOUCHAL, Pavel; Monika DVOŘÁKOVÁ; Theodoros ROUMELIOTIS; Zbyněk BORTLÍČEK; Iva STRUHÁROVÁ; Spiros D. GARBIS; Rudolf NENUTIL a Bořivoj VOJTĚŠEK. Towards understanding the mechanisms of metastasis in low grade breast cancer: Discovery of new targets via "omics" techniques. In HUPO 2011 10th Annual World Congress. 2011.

@proceedings{958924,
   author = {Bouchal, Pavel and Dvořáková, Monika and Roumeliotis, Theodoros and Bortlíček, Zbyněk and Struhárová, Iva and Garbis, Spiros D. and Nenutil, Rudolf and Vojtěšek, Bořivoj},
   booktitle = {HUPO 2011 10th Annual World Congress},
   keywords = {breast cancer, proteomics, transcriptomics},
   language = {eng},
   title = {Towards understanding the mechanisms of metastasis in low grade breast cancer: Discovery of new targets via "omics" techniques},
   url = {http://www.hupo2011.com},
   year = {2011}
}

TY  - CONF
ID  - 958924
AU  - Bouchal, Pavel - Dvořáková, Monika - Roumeliotis, Theodoros - Bortlíček, Zbyněk - Struhárová, Iva - Garbis, Spiros D. - Nenutil, Rudolf - Vojtěšek, Bořivoj
PY  - 2011
TI  - Towards understanding the mechanisms of metastasis in low grade breast cancer: Discovery of new targets via "omics" techniques
KW  - breast cancer, proteomics, transcriptomics
UR  - http://www.hupo2011.com
N2  - Background: Although breast cancer is the most common women cancer in the world, traditional prognostic factors are not sufficient for precise risk-group discrimination. This is well illustrated by the group of low grade breast tumors in which small percentage of tumors form lymph node metastases early. Methods: Our effort has been focused on biomarker discovery in the set of 96 breast cancer primary tumors grouped and randomized according to grade, lymph node and hormonal receptor status. iTRAQ-2DLC-MS/MS and 2-D SDSPAGE proteomics approaches were involved in complex protein analysis of tissue lysates. qRT-PCR assays and immunohistochemistry were used as complementary tools for verification of selected gene products expression. Results: Our results indicate several types of differences between the studied groups of tumors, namely: (i) iTRAQ- 2DLC-MS/MS data were internally validated via ErbB2 protein expression as confirmed by immunohistochemistry. (ii) Biological replicates in the study exhibited positive correlation and clustering of proteomic profiles according to clinicopathological parameters. (iii) Expression of selected cytoskeletal proteins correlated with lymph node status of the tumors. (iv) Expression of AGR family members was strongly affected by estrogene receptor status of the tumors. Conclusion: Our findings provide a solid data for targeted functional studies and validation geared towards more indepth description of metastatic mechanisms in low-grade breast cancer tumors.
ER  -

BOUCHAL, Pavel; Monika DVOŘÁKOVÁ; Theodoros ROUMELIOTIS; Zbyněk BORTLÍČEK; Iva STRUHÁROVÁ; Spiros D. GARBIS; Rudolf NENUTIL a Bořivoj VOJTĚŠEK. Towards understanding the mechanisms of metastasis in low grade breast cancer: Discovery of new targets via ''omics'' techniques. In \textit{HUPO 2011 10th Annual World Congress}. 2011.

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