| HAMAL, Petr, Juraj HANZEN, František HORN, Jitka TRTKOVÁ, Lenka RUSKOVÁ, Renata VEČEŘOVÁ, Filip RŮŽIČKA, Anna VOLLEKOVA a Vladislav RACLAVSKÝ. Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae. Online. Folia Microbiologica. 2011, roč. 56, č. 5, s. 407-414. ISSN 0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-011-0063-8. [citováno 2024-04-23] |
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@article{959068,
author = {Hamal, Petr and Hanzen, Juraj and Horn, František and Trtková, Jitka and Rusková, Lenka and Večeřová, Renata and Růžička, Filip and Vollekova, Anna and Raclavský, Vladislav},
article_number = {5},
doi = {http://dx.doi.org/10.1007/s12223-011-0063-8},
keywords = {STRAIN DELINEATION; ANTIFUNGAL SUSCEPTIBILITY; ALBICANS BIOFILMS; IDENTIFICATION; MENINGITIS; PATIENT; YEAST; EPIDEMIOLOGY; TRANSMISSION},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae},
volume = {56},
year = {2011}
}
TY - JOUR
ID - 959068
AU - Hamal, Petr - Hanzen, Juraj - Horn, František - Trtková, Jitka - Rusková, Lenka - Večeřová, Renata - Růžička, Filip - Vollekova, Anna - Raclavský, Vladislav
PY - 2011
TI - Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae
JF - Folia Microbiologica
VL - 56
IS - 5
SP - 407-414
EP - 407-414
SN - 00155632
KW - STRAIN DELINEATION
KW - ANTIFUNGAL SUSCEPTIBILITY
KW - ALBICANS BIOFILMS
KW - IDENTIFICATION
KW - MENINGITIS
KW - PATIENT
KW - YEAST
KW - EPIDEMIOLOGY
KW - TRANSMISSION
N2 - A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied retrospectively. To confirm identity, isolates were typed using pulsed-field gel electrophoresis and melting curve of random amplified polymorphic DNA (McRAPD). Further, the ability to form biofilm and its susceptibility to systemic antifungals were evaluated. Using McRAPD, identity of C. lusitaniae isolates showing slight microevolutionary changes in karyotypes was undoubtedly confirmed; successful application of numerical interpretation of McRAPD for typing is demonstrated here for the first time. The strain was also recognized as a strong biofilm producer. Moreover, minimum biofilm inhibitory concentrations were very high, in contrast to low antifungal minimum inhibitory concentrations of isolates. It can be concluded that McRAPD seems to be a simple and reliable method not only for identification but also for typing of yeasts. A ventriculoperitoneal shunt colonized by C. lusitaniae was revealed as the source of this nosocomial infection, and the ability of the strain to form biofilm on its surface likely caused treatment failure.
ER -
HAMAL, Petr, Juraj HANZEN, František HORN, Jitka TRTKOVÁ, Lenka RUSKOVÁ, Renata VEČEŘOVÁ, Filip RŮŽIČKA, Anna VOLLEKOVA a Vladislav RACLAVSKÝ. Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae. Online. \textit{Folia Microbiologica}. 2011, roč.~56, č.~5, s.~407-414. ISSN~0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-011-0063-8. [citováno 2024-04-23]
|
