How is transgelin involved in the mechanism of breast cancer
metastasis?

a 2011

How is transgelin involved in the mechanism of breast cancer metastasis?

DVOŘÁKOVÁ, Monika; Pavel BOUCHAL; Petr MÜLLER; Alexander SCHERL; Rudolf NENUTIL et al.

Základní údaje

Originální název

How is transgelin involved in the mechanism of breast cancer metastasis?

Autoři

DVOŘÁKOVÁ, Monika; Pavel BOUCHAL; Petr MÜLLER; Alexander SCHERL; Rudolf NENUTIL a Bořivoj VOJTĚŠEK

Vydání

„2nd RECAMO joint meeting“: Role of p53, MDM2, AGR2/3 and ubiquitin/chaperone system in tumour biology, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-86793-20-7

Klíčová slova česky

transgelin, breast cancer, metastasis

Klíčová slova anglicky

transgelin, breast cancer, metastasis

Příznaky

Mezinárodní význam

Změněno: 19. 12. 2011 20:49, doc. Mgr. Pavel Bouchal, Ph.D.

Anotace

V originále

The main challenge in breast cancer treatment is a metastatic disease. For estimation of the risk of metastatic disease, traditional prognostic factors like tumor size, tumor grade or lymph node status are currently used. However, the predictive value of these factors is not sufficient and there is an urgent need for new more reliable molecular markers with higher sensitivity and selectivity. Using a comparative proteomic approach, we identified transgelin as protein with increased expression in lymph-node positive low grade breast tumors compared to non-metastatic ones. This altered expression was further confirmed on transcriptional level using quantitative real-time PCR with absolute quantitation. Transgelin has been largely studied as an abundant protein of smooth muscle cells. With further development of proteomics and progress in the field of biomarker discovery, there is increasing number of studies in which altered expression of transgelin in tumor samples is described. Transgelin is thus, in all probability, involved in the process of tumor development. However, the results from the studies are often contradictory, even in the same tumor type and there are missing studies describing its function in these processes. With the aim to clarify the role of transgelin in breast cancer metastasis, we used a panel of breast cancer tissues with different characteristics and metastatic potential. Using siRNA silencing of transgelin expression, we characterized its effect on migration features of tumor cells. We also focused on the identification of protein-protein interactions of this protein using combination of pull-down approach with a quantitative proteomics based on stable isotope labeling with amino acids in cell cultures (SILAC). The samples were measured on a high-resolution mass spectrometry instrument (Orbitrap Velos) and the data were evaluated using MaxQuant software. Our results contribute to understanding the complex field of breast cancer metastasis and indicate new directions of our further experiments.

Návaznosti

GAP304/10/0868, projekt VaV

Název: Studium molekulárních mechanismů časného metastazování do lymfatických uzlin u karcinomu prsu nízkého stupně malignity pomocí proteomických technik

Investor: Grantová agentura ČR, Studium molekulárních mechanismů časného metastazování do lymfatických uzlin u karcinomu prsu nízkého stupně malignity pomocí proteomických technik

MZ0MOU2005, záměr

Název: Funkční diagnostika zhoubných nádorů

Citovat

DVOŘÁKOVÁ, Monika; Pavel BOUCHAL; Petr MÜLLER; Alexander SCHERL; Rudolf NENUTIL a Bořivoj VOJTĚŠEK. How is transgelin involved in the mechanism of breast cancer metastasis? In „2nd RECAMO joint meeting“: Role of p53, MDM2, AGR2/3 and ubiquitin/chaperone system in tumour biology. 2011. ISBN 978-80-86793-20-7.

@proceedings{960209,
   author = {Dvořáková, Monika and Bouchal, Pavel and Müller, Petr and Scherl, Alexander and Nenutil, Rudolf and Vojtěšek, Bořivoj},
   booktitle = {„2nd RECAMO joint meeting“: Role of p53, MDM2, AGR2/3 and ubiquitin/chaperone system in tumour biology},
   keywords = {transgelin, breast cancer, metastasis},
   language = {eng},
   isbn = {978-80-86793-20-7},
   title = {How is transgelin involved in the mechanism of breast cancer metastasis?},
   year = {2011}
}

TY  - CONF
ID  - 960209
AU  - Dvořáková, Monika - Bouchal, Pavel - Müller, Petr - Scherl, Alexander - Nenutil, Rudolf - Vojtěšek, Bořivoj
PY  - 2011
TI  - How is transgelin involved in the mechanism of breast cancer metastasis?
SN  - 9788086793207
KW  - transgelin, breast cancer, metastasis
N2  - The main challenge in breast cancer treatment is a metastatic disease. For estimation of the risk of metastatic disease, traditional prognostic factors like tumor size, tumor grade or lymph node status are currently used. However, the predictive value of these factors is not sufficient and there is an urgent need for new more reliable molecular markers with higher sensitivity and selectivity. Using a comparative proteomic approach, we identified transgelin as protein with increased expression in lymph-node positive low grade breast tumors compared to non-metastatic ones. This altered expression was further confirmed on transcriptional level using quantitative real-time PCR with absolute quantitation. Transgelin has been largely studied as an abundant protein of smooth muscle cells. With further development of proteomics and progress in the field of biomarker discovery, there is increasing number of studies in which altered expression of transgelin in tumor samples is described. Transgelin is thus, in all probability, involved in the process of tumor development. However, the results from the studies are often contradictory, even in the same tumor type and there are missing studies describing its function in these processes. With the aim to clarify the role of transgelin in breast cancer metastasis, we used a panel of breast cancer tissues with different characteristics and metastatic potential. Using siRNA silencing of transgelin expression, we characterized its effect on migration features of tumor cells. We also focused on the identification of protein-protein interactions of this protein using combination of pull-down approach with a quantitative proteomics based on stable isotope labeling with amino acids in cell cultures (SILAC). The samples were measured on a high-resolution mass spectrometry instrument (Orbitrap Velos) and the data were evaluated using MaxQuant software. Our results contribute to understanding the complex field of breast cancer metastasis and indicate new directions of our further experiments.
ER  -

DVOŘÁKOVÁ, Monika; Pavel BOUCHAL; Petr MÜLLER; Alexander SCHERL; Rudolf NENUTIL a Bořivoj VOJTĚŠEK. How is transgelin involved in the mechanism of breast cancer metastasis? In \textit{„2nd RECAMO joint meeting“: Role of p53, MDM2, AGR2/3 and ubiquitin/chaperone system in tumour biology}. 2011. ISBN~978-80-86793-20-7.

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