Experimental Administration of the Probiotic Escherichia coli
Strain Nissle 1917 Results in Decreased Diversity of E. coli
Strains in Pigs

J 2012

Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs

ŠMAJS, David; Jan BUREŠ; Jan ŠMARDA; Eva CHALOUPKOVÁ; Jaroslav KVĚTINA et al.

Základní údaje

Originální název

Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs

Autoři

ŠMAJS, David; Jan BUREŠ; Jan ŠMARDA; Eva CHALOUPKOVÁ; Jaroslav KVĚTINA; Miroslav FÖRSTL; Darina KOHOUTOVÁ; Martin KUNEŠ; Stanislav REJCHRT; Jiřina LESNÁ a Marcela KOPÁČOVÁ

Vydání

Current Microbiology, 2012, 0343-8651

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.520

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/12:00059148

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1007/s00284-011-0051-x

UT WoS

000300769800001

Klíčová slova anglicky

Escherichia coli; pig; strain Escherichia coli Nissle 1917

Příznaky

Mezinárodní význam

Změněno: 23. 3. 2012 14:07, Mgr. Michal Petr

Anotace

V originále

The strain Escherichia coli Nissle 1917 (EcN) is widely used as an efficient probiotic in therapy and prevention of human infectious diseases, especially of the intestinal system. Concurrently, small adult pigs are being used as experimental omnivore models to study human gastrointestinal functions. EcN bacteria were applied to 6 adult healthy female pigs in a 2-week trial. 6 Control animals remained untreated. Altogether, 164 and 149 bacterial strains were isolated from smear samples taken from gastrointestinal mucosa in the experimental and control group, respectively. Each individual E. coli strain was then tested for the presence of 29 bacteriocin-encoding determinants as well as for DNA markers of A, B1, B2 and D phylogenetic groups. A profound reduction of E. coli genetic variance (from 32 variants to 13 ones, P = 0.0006) was found in the experimental group, accompanied by a lower incidence of bacteriocin producers in the experimental group when compared to control (21.3 and 34.9%, respectively; P = 0.007) and by changes in the incidence of individual bacteriocin types. The experimental administration of EcN strain was not sufficient for stable colonization of porcine gut, but induced significant changes in the enterobacterial microbiota.

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

NS9665, projekt VaV

Název: Molekulární typizace bakteriocinů produkovaných kmeny Escherichia coli z klinického materiálu: identifikace kolicinů a mikrocinů v souborech komensálních a patogenních kmenů

Investor: Ministerstvo zdravotnictví ČR, Molekulární typizace bakteriocinů produkovaných kmeny Escherichia coli z klinického materiálu: identifikace kolicinů a mikrocinů v souborech komensálních a patogenních kmenů

Citovat

ŠMAJS, David; Jan BUREŠ; Jan ŠMARDA; Eva CHALOUPKOVÁ; Jaroslav KVĚTINA; Miroslav FÖRSTL; Darina KOHOUTOVÁ; Martin KUNEŠ; Stanislav REJCHRT; Jiřina LESNÁ a Marcela KOPÁČOVÁ. Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs. Current Microbiology. 2012, roč. 64, č. 3, s. 205-210. ISSN 0343-8651. Dostupné z: https://doi.org/10.1007/s00284-011-0051-x.

@article{961061,
   author = {Šmajs, David and Bureš, Jan and Šmarda, Jan and Chaloupková, Eva and Květina, Jaroslav and Förstl, Miroslav and Kohoutová, Darina and Kuneš, Martin and Rejchrt, Stanislav and Lesná, Jiřina and Kopáčová, Marcela},
   article_number = {3},
   doi = {https://doi.org/10.1007/s00284-011-0051-x},
   keywords = {Escherichia coli; pig; strain Escherichia coli Nissle 1917},
   language = {eng},
   issn = {0343-8651},
   journal = {Current Microbiology},
   title = {Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs},
   volume = {64},
   year = {2012}
}

TY  - JOUR
ID  - 961061
AU  - Šmajs, David - Bureš, Jan - Šmarda, Jan - Chaloupková, Eva - Květina, Jaroslav - Förstl, Miroslav - Kohoutová, Darina - Kuneš, Martin - Rejchrt, Stanislav - Lesná, Jiřina - Kopáčová, Marcela
PY  - 2012
TI  - Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs
JF  - Current Microbiology
VL  - 64
IS  - 3
SP  - 205-210
EP  - 205-210
SN  - 03438651
KW  - Escherichia coli
KW  - pig
KW  - strain Escherichia coli Nissle 1917
N2  - The strain Escherichia coli Nissle 1917 (EcN) is widely used as an efficient probiotic in therapy and prevention of human infectious diseases, especially of the intestinal system. Concurrently, small adult pigs are being used as experimental omnivore models to study human gastrointestinal functions. EcN bacteria were applied to 6 adult healthy female pigs in a 2-week trial. 6 Control animals remained untreated. Altogether, 164 and 149 bacterial strains were isolated from smear samples taken from gastrointestinal mucosa in the experimental and control group, respectively. Each individual E. coli strain was then tested for the presence of 29 bacteriocin-encoding determinants as well as for DNA markers of A, B1, B2 and D phylogenetic groups. A profound reduction of E. coli genetic variance (from 32 variants to 13 ones, P = 0.0006) was found in the experimental group, accompanied by a lower incidence of bacteriocin producers in the experimental group when compared to control (21.3 and 34.9%, respectively; P = 0.007) and by changes in the incidence of individual bacteriocin types. The experimental administration of EcN strain was not sufficient for stable colonization of porcine gut, but induced significant changes in the enterobacterial microbiota.
ER  -

ŠMAJS, David; Jan BUREŠ; Jan ŠMARDA; Eva CHALOUPKOVÁ; Jaroslav KVĚTINA; Miroslav FÖRSTL; Darina KOHOUTOVÁ; Martin KUNEŠ; Stanislav REJCHRT; Jiřina LESNÁ a Marcela KOPÁČOVÁ. Experimental Administration of the Probiotic Escherichia coli Strain Nissle 1917 Results in Decreased Diversity of E. coli Strains in Pigs. \textit{Current Microbiology}. 2012, roč.~64, č.~3, s.~205-210. ISSN~0343-8651. Dostupné z: https://doi.org/10.1007/s00284-011-0051-x.

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