The interplay of the aryl hydrocarbon receptor and beta-catenin
alters both AhR-dependent transcription and Wnt/beta catenin
signaling in liver progenitors.

J 2011

The interplay of the aryl hydrocarbon receptor and beta-catenin alters both AhR-dependent transcription and Wnt/beta catenin signaling in liver progenitors.

PROCHÁZKOVÁ, Jiřina; Markéta KABÁTKOVÁ; Vítězslav BRYJA; Lenka UMANNOVÁ; Ondřej BERNATÍK et al.

Základní údaje

Originální název

The interplay of the aryl hydrocarbon receptor and beta-catenin alters both AhR-dependent transcription and Wnt/beta catenin signaling in liver progenitors.

Autoři

PROCHÁZKOVÁ, Jiřina; Markéta KABÁTKOVÁ; Vítězslav BRYJA; Lenka UMANNOVÁ ; Ondřej BERNATÍK; Alois KOZUBÍK; Miroslav MACHALA a Jan VONDRÁČEK

Vydání

Toxicological sciences, 2011, 1096-6080

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.652

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/11:00050224

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1093/toxsci/kfr129

UT WoS

000293914500011

Klíčová slova anglicky

Wnt signaling; dioxin; intercellular junctions; contact inhibition; liver progenitor cells; differentiation

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 6. 2014 13:15, Mgr. Markéta Kabátková, Ph.D.

Anotace

V originále

beta-catenin is a key integrator of cadherin-mediated cell-cell adhesion and transcriptional regulation through the Wnt/beta-catenin pathway, which plays an important role in liver biology. Using a model of contact-inhibited liver progenitor cells, we examined the interactions of Wnt/beta-catenin signaling with the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor, which mediates the toxicity of dioxin-like compounds, including their effects on development and hepatocarcinogenesis. We found that AhR and Wnt/beta-catenin cooperated in the induction of AhR transcriptional targets, such as Cyp1a1 and Cyp1b1. However, simultaneously, the activation of AhR led to a decrease of dephosphorylated active beta-catenin pool, as well as to hypophosphorylation of Dishevelled, participating in regulation of Wnt signaling. A sustained AhR activation by its model ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), led to a downregulation of a number of Wnt/beta-catenin pathway target genes. TCDD also induced a switch in cytokeratin expression, where downregulation of cytokeratins 14 and 19 was accompanied with an increased cytokeratin 8 expression. Together with a downregulation of additional markers associated with stem-like phenotype, this indicated that the AhR activation interfered with differentiation of liver progenitors.

Návaznosti

GD204/09/H058, projekt VaV

Název: Mezibuněčná signalizace ve vývoji organismu a vzniku onemocnění

Investor: Grantová agentura ČR, Mezibuněčná signalizace ve vývoji organismu a vzniku onemocnění

MSM0021622430, záměr

Název: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Citovat

PROCHÁZKOVÁ, Jiřina; Markéta KABÁTKOVÁ; Vítězslav BRYJA; Lenka UMANNOVÁ; Ondřej BERNATÍK; Alois KOZUBÍK; Miroslav MACHALA a Jan VONDRÁČEK. The interplay of the aryl hydrocarbon receptor and beta-catenin alters both AhR-dependent transcription and Wnt/beta catenin signaling in liver progenitors. Toxicological sciences. 2011, roč. 122, č. 2, s. 349-360. ISSN 1096-6080. Dostupné z: https://doi.org/10.1093/toxsci/kfr129.

@article{962162,
   author = {Procházková, Jiřina and Kabátková, Markéta and Bryja, Vítězslav and Umannová, Lenka and Bernatík, Ondřej and Kozubík, Alois and Machala, Miroslav and Vondráček, Jan},
   article_number = {2},
   doi = {https://doi.org/10.1093/toxsci/kfr129},
   keywords = {Wnt signaling; dioxin; intercellular junctions; contact inhibition; liver progenitor cells; differentiation},
   language = {eng},
   issn = {1096-6080},
   journal = {Toxicological sciences},
   title = {The interplay of the aryl hydrocarbon receptor and beta-catenin alters both AhR-dependent transcription and Wnt/beta catenin signaling in liver progenitors.},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/21602191},
   volume = {122},
   year = {2011}
}

TY  - JOUR
ID  - 962162
AU  - Procházková, Jiřina - Kabátková, Markéta - Bryja, Vítězslav - Umannová, Lenka - Bernatík, Ondřej - Kozubík, Alois - Machala, Miroslav - Vondráček, Jan
PY  - 2011
TI  - The interplay of the aryl hydrocarbon receptor and beta-catenin alters both AhR-dependent transcription and Wnt/beta catenin signaling in liver progenitors.
JF  - Toxicological sciences
VL  - 122
IS  - 2
SP  - 349-360
EP  - 349-360
SN  - 10966080
KW  - Wnt signaling
KW  - dioxin
KW  - intercellular junctions
KW  - contact inhibition
KW  - liver progenitor cells
KW  - differentiation
UR  - http://www.ncbi.nlm.nih.gov/pubmed/21602191
L2  - http://www.ncbi.nlm.nih.gov/pubmed/21602191
N2  - beta-catenin is a key integrator of cadherin-mediated cell-cell adhesion and transcriptional regulation through the Wnt/beta-catenin pathway, which plays an important role in liver biology. Using a model of contact-inhibited liver progenitor cells, we examined the interactions of Wnt/beta-catenin signaling with the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor, which mediates the toxicity of dioxin-like compounds, including their effects on development and hepatocarcinogenesis. We found that AhR and Wnt/beta-catenin cooperated in the induction of AhR transcriptional targets, such as Cyp1a1 and Cyp1b1. However, simultaneously, the activation of AhR led to a decrease of dephosphorylated active beta-catenin pool, as well as to hypophosphorylation of Dishevelled, participating in regulation of Wnt signaling. A sustained AhR activation by its model ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), led to a downregulation of a number of Wnt/beta-catenin pathway target genes. TCDD also induced a switch in cytokeratin expression, where downregulation of cytokeratins 14 and 19 was accompanied with an increased cytokeratin 8 expression. Together with a downregulation of additional markers associated with stem-like phenotype, this indicated that the AhR activation interfered with differentiation of liver progenitors.
ER  -

PROCHÁZKOVÁ, Jiřina; Markéta KABÁTKOVÁ; Vítězslav BRYJA; Lenka UMANNOVÁ; Ondřej BERNATÍK; Alois KOZUBÍK; Miroslav MACHALA a Jan VONDRÁČEK. The interplay of the aryl hydrocarbon receptor and beta-catenin alters both AhR-dependent transcription and Wnt/beta catenin signaling in liver progenitors. \textit{Toxicological sciences}. 2011, roč.~122, č.~2, s.~349-360. ISSN~1096-6080. Dostupné z: https://doi.org/10.1093/toxsci/kfr129.

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