2011
THE NUCLEAR PORE COMPLEXES CHANGED AFTER ULTRASOUND EXPOSURE
VAŠKOVICOVÁ, Naděžda, Roman JANISCH a Jiřina ŠKORPÍKOVÁZákladní údaje
Originální název
THE NUCLEAR PORE COMPLEXES CHANGED AFTER ULTRASOUND EXPOSURE
Název anglicky
THE NUCLEAR PORE COMPLEXES CHANGED AFTER ULTRASOUND EXPOSURE
Autoři
VAŠKOVICOVÁ, Naděžda, Roman JANISCH a Jiřina ŠKORPÍKOVÁ
Vydání
XX. Biologické dny, 2011
Další údaje
Typ výsledku
Konferenční abstrakt
Utajení
není předmětem státního či obchodního tajemství
ISBN
978-80-260-0849-1
Změněno: 16. 12. 2011 12:31, Mgr. Naděžda Vaškovicová, Ph.D.
Anotace
V originále
Changes in the size of diameter of nuclear pore complexes fractured along a protoplasmic and an exoplasmic face of the inner and outer membrane of a nuclear envelope (freeze-etching method) in HL-60 cells after ultrasound exposure were studied. A cell suspension was exposed at 1 MHz frequency and 1 W/cm2 intensity in a continuous mode for 10 min was placed in a 37 degrees Celsius water bath; samples were collected at four different intervals (0 min, 10 min, 20 min, 30 min) and fixed in 2 % paraformaldehyde + 2.5 % glutaraldehyde in 0.1 M cacodylate buffer. The assessment showed significant differences in the size of diameter of nuclear pore complexes on the protoplasmic face between ultrasound-exposed and unexposed cells (P0=0.001, P10=0.000, P20=0.000). On the exoplasmic face there was a significant difference between ultrasound-exposed and unexposed cells only in the sample fixed immediately after sonication (P0=0.004). In the other samples the differences were not statistically significant. The study shows that therapeutic ultrasound has an effect on cellular structures formed from subunits and separate proteins whose bonds can be disintegrated by mechanical waves. The visual manifestation of it is an increase in the size of diameter of nuclear pore complexes on fractured membrane faces.