Detection and quantification of bacterial genes of
Staphylococcus aureus in transducing phage particles by qPCR

a 2011

Detection and quantification of bacterial genes of Staphylococcus aureus in transducing phage particles by qPCR

MAŠLAŇOVÁ, Ivana, Marian VARGA, Lucie KUNTOVÁ, Roman PANTŮČEK, Jiří DOŠKAŘ et. al.

Základní údaje

Originální název

Detection and quantification of bacterial genes of Staphylococcus aureus in transducing phage particles by qPCR

Název česky

Detekce a kvantifikace bakteriálních genů druhu Staphylococcus aureus v transdukujících fágových částicích pomocí qPCR

Autoři

MAŠLAŇOVÁ, Ivana, Marian VARGA, Lucie KUNTOVÁ, Roman PANTŮČEK a Jiří DOŠKAŘ

Vydání

Federation of Infection Societies Conference 2011, Manchester, UK, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Staphylococcus; aureus; kvantifikace; transdukce; plazmid; bakteriofág; rezistence; antibiotikum; qPCR

Klíčová slova anglicky

Staphylococcus; aureus; quantification; transduction; plasmid; bacteriophage; resistance; antibiotic; qPCR

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 1. 2012 09:19, Mgr. Ivana Mašlaňová, Ph.D.

Anotace

V originále

Staphylococcus aureus is a major human and veterinary pathogen causing local and systemic infections. The pathogenicity of S. aureus depends on production of virulent factors and contents of resistant determinants to antimicrobial agents. These virulent and resistant factors are carried on mobile genetic elements (MGE). Different types of MGE comprise 15 % of genome S. aureus and can be horizontal transferred. The transduction mediated by temperate staphylococcal bacteriophages of serological group B is one of the horizontal gene transfer mechanisms.The aim of the project is detection and quantification of bacterial genes on MGE, which are transferred by transducing phage particles. The work is focused on staphylococcal chromosomal cassettes (SCCmec), pathogenicity islands (SaPIs), plasmids and chromosomal genes encoding virulent factors. qPCR was used for the quantification and detection of genes. It is more sensitive and has higher resolution level than end-point PCR. The plasmid copy number in S. aureus, resistant genes on plasmids, the parts of SCCmec and SaPIs in transducing phage particles were determined by qPCR. The results of this project contribute to explanation of mechanism and frequency of horizontal gene transfer by transducing phage particles.

Návaznosti

GA310/09/0459, projekt VaV

Název: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

Investor: Grantová agentura ČR, Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

MAŠLAŇOVÁ, Ivana, Marian VARGA, Lucie KUNTOVÁ, Roman PANTŮČEK a Jiří DOŠKAŘ. Detection and quantification of bacterial genes of Staphylococcus aureus in transducing phage particles by qPCR. In Federation of Infection Societies Conference 2011, Manchester, UK. 2011.

@proceedings{965322,
   author = {Mašlaňová, Ivana and Varga, Marian and Kuntová, Lucie and Pantůček, Roman and Doškař, Jiří},
   booktitle = {Federation of Infection Societies Conference 2011, Manchester, UK},
   keywords = {Staphylococcus; aureus; quantification; transduction; plasmid; bacteriophage; resistance; antibiotic; qPCR},
   language = {eng},
   title = {Detection and quantification of bacterial genes of Staphylococcus aureus in transducing phage particles by qPCR},
   url = {http://www.fis-infection.org.uk/},
   year = {2011}
}

TY  - CONF
ID  - 965322
AU  - Mašlaňová, Ivana - Varga, Marian - Kuntová, Lucie - Pantůček, Roman - Doškař, Jiří
PY  - 2011
TI  - Detection and quantification of bacterial genes of Staphylococcus aureus in transducing phage particles by qPCR
KW  - Staphylococcus
KW  - aureus
KW  - quantification
KW  - transduction
KW  - plasmid
KW  - bacteriophage
KW  - resistance
KW  - antibiotic
KW  - qPCR
UR  - http://www.fis-infection.org.uk/
N2  - Staphylococcus aureus is a major human and veterinary pathogen causing local and systemic infections. The pathogenicity of S. aureus depends on production of virulent factors and contents of resistant determinants to antimicrobial agents. These virulent and resistant factors are carried on mobile genetic elements (MGE). Different types of MGE comprise 15 % of genome S. aureus and can be horizontal transferred. The transduction mediated by temperate staphylococcal bacteriophages of serological group B is one of the horizontal gene transfer mechanisms.The aim of the project is detection and quantification of bacterial genes on MGE, which are transferred by transducing phage particles. The work is focused on staphylococcal chromosomal cassettes (SCCmec), pathogenicity islands (SaPIs), plasmids and chromosomal genes encoding virulent factors. qPCR was used for the quantification and detection of genes. It is more sensitive and has higher resolution level than end-point PCR. The plasmid copy number in S. aureus, resistant genes on plasmids, the parts of SCCmec and SaPIs in transducing phage particles were determined by qPCR. The results of this project contribute to explanation of mechanism and frequency of horizontal gene transfer by transducing phage particles.
ER  -

MAŠLAŇOVÁ, Ivana, Marian VARGA, Lucie KUNTOVÁ, Roman PANTŮČEK a Jiří DOŠKAŘ. Detection and quantification of bacterial genes of Staphylococcus aureus in transducing phage particles by qPCR. In \textit{Federation of Infection Societies Conference 2011, Manchester, UK}. 2011.

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