J 2011

Depleted genetic variation of the European ground squirrel in Central Europe in both microsatellites and the major histocompatibility complex gene: implications for conservation

ŘÍČANOVÁ, Štěpánka; Josef BRYJA; Jean Francois COSSON; Csongor GEDEON; Lukáš CHOLEVA et al.

Základní údaje

Originální název

Depleted genetic variation of the European ground squirrel in Central Europe in both microsatellites and the major histocompatibility complex gene: implications for conservation

Název česky

Depleted genetic variation of the European ground squirrel in Central Europe in both microsatellites and the major histocompatibility complex gene: implications for conservation

Autoři

ŘÍČANOVÁ, Štěpánka; Josef BRYJA; Jean Francois COSSON; Csongor GEDEON; Lukáš CHOLEVA; Michal AMBROS a František SEDLÁČEK

Vydání

Conservation Genetics, Netherlands, Springer, 2011, 1566-0621

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.610

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/11:00054809

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Souslik; Endangered species; Sciuridae; Habitat fragmentation; DRB; MHC Class II

Klíčová slova anglicky

Souslik; Endangered species; Sciuridae; Habitat fragmentation; DRB; MHC Class II

Štítky

Změněno: 10. 1. 2012 18:08, doc. RNDr. Zdeněk Řehák, Ph.D.

Anotace

V originále

Habitat fragmentation may influence the genetic make-up and adaptability of endangered populations. To facilitate genetic monitoring of the endangered European ground squirrel (EGS), we analyzed 382 individuals from 16 populations in Central Europe, covering almost half of its natural range. We tested how fragmentation affects the genetic architecture of presumably selectively neutral (12 microsatellites) and non-neutral (the major histocompatibility class II DRB gene) loci. Spatial genetic analyses defined two groups of populations, ‘‘western’’ and ‘‘eastern’’, with a significantly higher level of habitat fragmentation in the former group. The highly fragmented western populations had significantly lower genetic diversity in both types of markers. Only one allele of the DRB gene predominated in populations of the western group, while four alleles were evenly distributed across the eastern populations. Coefficient of inbreeding values (FIS) calculated from microsatellites were significantly higher in the western (0.27–0.79) than in eastern populations (-0.060–0.119). Inter-population differentiation was very high, but similar in both groups (western FST = 0.23, eastern FST = 0.25). The test of isolation by distance was significant for the whole dataset, as well as for the two groups analyzed separately. Comparison of genetic variability and structure on microsatellites and the DRB gene does not provide any evidence for contemporary selection on MHC genes. We suggest that genetic drift in small bottlenecked and fragmented populations may overact the role of balancing selection. Based on the resulting risk of inbreeding depression in the western populations, we support population management by crossbreeding between the western and eastern populations.

Česky

Habitat fragmentation may influence the genetic make-up and adaptability of endangered populations. To facilitate genetic monitoring of the endangered European ground squirrel (EGS), we analyzed 382 individuals from 16 populations in Central Europe, covering almost half of its natural range. We tested how fragmentation affects the genetic architecture of presumably selectively neutral (12 microsatellites) and non-neutral (the major histocompatibility class II DRB gene) loci. Spatial genetic analyses defined two groups of populations, ‘‘western’’ and ‘‘eastern’’, with a significantly higher level of habitat fragmentation in the former group. The highly fragmented western populations had significantly lower genetic diversity in both types of markers. Only one allele of the DRB gene predominated in populations of the western group, while four alleles were evenly distributed across the eastern populations. Coefficient of inbreeding values (FIS) calculated from microsatellites were significantly higher in the western (0.27–0.79) than in eastern populations (-0.060–0.119). Inter-population differentiation was very high, but similar in both groups (western FST = 0.23, eastern FST = 0.25). The test of isolation by distance was significant for the whole dataset, as well as for the two groups analyzed separately. Comparison of genetic variability and structure on microsatellites and the DRB gene does not provide any evidence for contemporary selection on MHC genes. We suggest that genetic drift in small bottlenecked and fragmented populations may overact the role of balancing selection. Based on the resulting risk of inbreeding depression in the western populations, we support population management by crossbreeding between the western and eastern populations.

Návaznosti

MSM0021622416, záměr
Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase