Amplification of hTERC (3q26) and MYCC (8q24) genes in
correlation with oncogenic human papilloma virus infection in
cervical cancer

a 2011

Amplification of hTERC (3q26) and MYCC (8q24) genes in correlation with oncogenic human papilloma virus infection in cervical cancer

VRANOVÁ, Vladimíra; Lucie MOUKOVÁ; Miroslava KIŠŠOVÁ; Iva SLÁMOVÁ; Petr KUGLÍK et al.

Základní údaje

Originální název

Amplification of hTERC (3q26) and MYCC (8q24) genes in correlation with oncogenic human papilloma virus infection in cervical cancer

Autoři

VRANOVÁ, Vladimíra; Lucie MOUKOVÁ; Miroslava KIŠŠOVÁ; Iva SLÁMOVÁ a Petr KUGLÍK

Vydání

European Cytogenetics Conference, Porto, Portugal, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.087

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/11:00055260

Organizační jednotka

Přírodovědecká fakulta

ISSN

Klíčová slova anglicky

cervix uteri; hTERC; MYCC; FISH; papiloma virus

Změněno: 1. 2. 2012 10:44, RNDr. Vladimíra Vallová, Ph.D.

Anotace

V originále

Cervical cancer remains one of the most common malignancies among women worldwide in both incidence and mortality. Several premalignant stages can be distinguished in the development of invasive carcinoma, including cervical intraepithelial neoplasia grades I-III (CIN I–III). The genetic basis of progression of CIN I to CIN II/III and to invasive carcinoma remain poorly understood. Although infection of high-risk human papilloma virus (HPV) is recognized as an essential initiating event in cervical tumorigenesis, this alone is not sufficient for the progression to invasive cancer. Some chromosomal aberrations have been associated with the progression of CIN to carcinoma, especially the amplification of the human telomerase gene hTERC (3q26) and protooncogene MYCC (8q24). Recently, Cervical FISH Probe Kit (Abbott-Vysis) was designed for common identification of HPV infected cells and copy number aberration of the hTERC a MYCC genes via fluorescence in situ hybridization (FISH) in cervical cytology specimens. In this study, cytology specimens of cervical carcinoma or cervical intraepithelial neoplasia from exocervix and endocervical canal of 20 women were analyzed using the new Cervical FISH Probe Kit. We determined number of HPV positive cells, status of HPV (episomal or integrated), copy number changes of chromosome hTERC and MYCC gene in all samples and compare the findings with the cytologic and histologic diagnoses. In some cases, oligonucleotide array based comparative genomic hybridization (aCGH) from fresh tumour was done to confirm the FISH findings and to find some more chromosomal changes associated with cervical cancer.

Návaznosti

NT11089-4/2010, interní kód MU

Název: Diagnostický význam amplifikace genů hTERC a MYCC při vzniku a vývoji cervikálních intraepiteliálních dysplázií a karcinomu děložního hrdla

Investor: Ministerstvo zdravotnictví ČR, Diagnostický význam amplifikace genů hTERC a MYCC při vzniku a vývoji cervikálních intraepiteliálních dysplázií a karcinomu děložního hrdla

Citovat

VRANOVÁ, Vladimíra; Lucie MOUKOVÁ; Miroslava KIŠŠOVÁ; Iva SLÁMOVÁ a Petr KUGLÍK. Amplification of hTERC (3q26) and MYCC (8q24) genes in correlation with oncogenic human papilloma virus infection in cervical cancer. In European Cytogenetics Conference, Porto, Portugal. 2011. ISSN 0967-3849.

@proceedings{969068,
   author = {Vranová, Vladimíra and Mouková, Lucie and Kiššová, Miroslava and Slámová, Iva and Kuglík, Petr},
   booktitle = {European Cytogenetics Conference, Porto, Portugal},
   keywords = {cervix uteri; hTERC; MYCC; FISH; papiloma virus},
   language = {eng},
   title = {Amplification of hTERC (3q26) and MYCC (8q24) genes in correlation with oncogenic human papilloma virus infection in cervical cancer},
   year = {2011}
}

TY  - CONF
ID  - 969068
AU  - Vranová, Vladimíra - Mouková, Lucie - Kiššová, Miroslava - Slámová, Iva - Kuglík, Petr
PY  - 2011
TI  - Amplification of hTERC (3q26) and MYCC (8q24) genes in correlation with oncogenic human papilloma virus infection in cervical cancer
KW  - cervix uteri
KW  - hTERC
KW  - MYCC
KW  - FISH
KW  - papiloma virus
N2  - Cervical cancer remains one of the most common malignancies among women worldwide in both incidence and mortality. Several premalignant stages can be distinguished in the development of invasive carcinoma, including cervical intraepithelial neoplasia grades I-III (CIN I–III). The genetic basis of progression of CIN I to CIN II/III and to invasive carcinoma remain poorly understood. Although infection of high-risk human papilloma virus (HPV) is recognized as an essential initiating event in cervical tumorigenesis, this alone is not sufficient for the progression to invasive cancer. Some chromosomal aberrations have been associated with the progression of CIN to carcinoma, especially the amplification of the human telomerase gene hTERC (3q26) and protooncogene MYCC (8q24). Recently, Cervical FISH Probe Kit (Abbott-Vysis) was designed for common identification of HPV infected cells and copy number aberration of the hTERC a MYCC genes via fluorescence in situ hybridization (FISH) in cervical cytology specimens. In this study, cytology specimens of cervical carcinoma or cervical intraepithelial neoplasia from exocervix and endocervical canal of 20 women were analyzed using the new Cervical FISH Probe Kit. We determined number of HPV positive cells, status of HPV (episomal or integrated), copy number changes of chromosome hTERC and MYCC gene in all samples and compare the findings with the cytologic and histologic diagnoses. In some cases, oligonucleotide array based comparative genomic hybridization (aCGH) from fresh tumour was done to confirm the FISH findings and to find some more chromosomal changes associated with cervical cancer.
ER  -

VRANOVÁ, Vladimíra; Lucie MOUKOVÁ; Miroslava KIŠŠOVÁ; Iva SLÁMOVÁ a Petr KUGLÍK. Amplification of hTERC (3q26) and MYCC (8q24) genes in correlation with oncogenic human papilloma virus infection in cervical cancer. In \textit{European Cytogenetics Conference, Porto, Portugal}. 2011. ISSN~0967-3849.

Přehled o publikaci