Expression of mRNA for Adenosine A1, A2a, A2b, and A3 Receptors
in HL-60 Cells: Dependence on Cell Cycle Phases

J 2011

Expression of mRNA for Adenosine A1, A2a, A2b, and A3 Receptors in HL-60 Cells: Dependence on Cell Cycle Phases

HOFER, M.; Ladislav DUŠEK; Z. HOFEROVÁ; L. STIXOVÁ; M. POSPÍŠIL et al.

Základní údaje

Originální název

Expression of mRNA for Adenosine A1, A2a, A2b, and A3 Receptors in HL-60 Cells: Dependence on Cell Cycle Phases

Autoři

HOFER, M.; Ladislav DUŠEK; Z. HOFEROVÁ; L. STIXOVÁ a M. POSPÍŠIL

Vydání

Physiological Research, 2011, 0862-8408

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.555

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/11:00050445

Organizační jednotka

Lékařská fakulta

UT WoS

000299021500009

Klíčová slova anglicky

Adenosine receptors; Messenger RNA expression; Cell cycle; HL-60 promyelocytic cells

Příznaky

Mezinárodní význam

Změněno: 1. 2. 2012 12:54, Mgr. Michal Petr

Anotace

V originále

The present studies investigated changes in expression of mRNA for adenosine A1, A2a, A2b, and A3 receptors in samples of HL-60 promyelocytic cells differing in the actual presence of cells in various phases of the cell cycle induced by the double thymidine block method. Real-time PCR technique was used for obtaining data on mRNA expression. Statistical analysis of the data revealed that the mRNA expression of adenosine A1, A2a, and A3 receptors is dependent on the cell cycle phase. G0/G1 and G2/M phases were characterized by a higher mRNA expression of adenosine A1 receptors and a lower one of adenosine A2a and A3 receptors whereas the opposite was true for the S phase. Interestingly, expression of mRNA of the adenosine A2b receptors was independent on the cell cycle phase. The results indicate the plasticity of mRNA expression of adenosine receptors in the investigated promyelocytic cells and its interaction with physiological mechanisms of the cell cycle.

Návaznosti

GA305/08/0158, projekt VaV

Název: Aktivace adenosinových receptorů kombinovaná s inhibicí cyklooxygenázy v modulaci zářením vyvolaného poškození kostní dřeně

Investor: Grantová agentura ČR, Aktivace adenosinových receptorů kompbinovaná s inhibicí cyklooxygenázy v modulaci zářením vyvolavaného poškození kostní dřeně

Citovat

HOFER, M.; Ladislav DUŠEK; Z. HOFEROVÁ; L. STIXOVÁ a M. POSPÍŠIL. Expression of mRNA for Adenosine A1, A2a, A2b, and A3 Receptors in HL-60 Cells: Dependence on Cell Cycle Phases. Physiological Research. 2011, roč. 60, č. 6, s. 913-920. ISSN 0862-8408.

@article{969118,
   author = {Hofer, M. and Dušek, Ladislav and Hoferová, Z. and Stixová, L. and Pospíšil, M.},
   article_number = {6},
   keywords = {Adenosine receptors; Messenger RNA expression; Cell cycle; HL-60 promyelocytic cells},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological Research},
   title = {Expression of mRNA for Adenosine A1, A2a, A2b, and A3 Receptors in HL-60 Cells: Dependence on Cell Cycle Phases},
   volume = {60},
   year = {2011}
}

TY  - JOUR
ID  - 969118
AU  - Hofer, M. - Dušek, Ladislav - Hoferová, Z. - Stixová, L. - Pospíšil, M.
PY  - 2011
TI  - Expression of mRNA for Adenosine A1, A2a, A2b, and A3 Receptors in HL-60 Cells: Dependence on Cell Cycle Phases
JF  - Physiological Research
VL  - 60
IS  - 6
SP  - 913-920
EP  - 913-920
SN  - 08628408
KW  - Adenosine receptors
KW  - Messenger RNA expression
KW  - Cell cycle
KW  - HL-60 promyelocytic cells
N2  - The present studies investigated changes in expression of mRNA for adenosine A1, A2a, A2b, and A3 receptors in samples of HL-60 promyelocytic cells differing in the actual presence of cells in various phases of the cell cycle induced by the double thymidine block method. Real-time PCR technique was used for obtaining data on mRNA expression. Statistical analysis of the data revealed that the mRNA expression of adenosine A1, A2a, and A3 receptors is dependent on the cell cycle phase. G0/G1 and G2/M phases were characterized by a higher mRNA expression of adenosine A1 receptors and a lower one of adenosine A2a and A3 receptors whereas the opposite was true for the S phase. Interestingly, expression of mRNA of the adenosine A2b receptors was independent on the cell cycle phase. The results indicate the plasticity of mRNA expression of adenosine receptors in the investigated promyelocytic cells and its interaction with physiological mechanisms of the cell cycle.
ER  -

HOFER, M.; Ladislav DUŠEK; Z. HOFEROVÁ; L. STIXOVÁ a M. POSPÍŠIL. Expression of mRNA for Adenosine A1, A2a, A2b, and A3 Receptors in HL-60 Cells: Dependence on Cell Cycle Phases. \textit{Physiological Research}. 2011, roč.~60, č.~6, s.~913-920. ISSN~0862-8408.

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