| GUMULEC, Jaromír, Michal MASAŘÍK, Soňa KŘÍŽKOVÁ, Marián HLAVNA, P. BABULA, Roman HRABEC, Arne ROVNÝ, Miroslava MASAŘÍKOVÁ, J. SOCHOR, V. ADAM, T. ECKSCHLAGER a René KIZEK. Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers. Neoplasma. 2012, roč. 59, č. 2, s. 191-200. ISSN 0028-2685. Dostupné z: https://dx.doi.org/10.4149/neo_2012_025. |
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@article{970027,
author = {Gumulec, Jaromír and Masařík, Michal and Křížková, Soňa and Hlavna, Marián and Babula, P. and Hrabec, Roman and Rovný, Arne and Masaříková, Miroslava and Sochor, J. and Adam, V. and Eckschlager, T. and Kizek, René},
article_number = {2},
doi = {http://dx.doi.org/10.4149/neo_2012_025},
keywords = {cancer; tumor marker; immunodetection; electrochemistry; polymerase chain reaction; mRNA},
language = {eng},
issn = {0028-2685},
journal = {Neoplasma},
title = {Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers},
volume = {59},
year = {2012}
}
TY - JOUR
ID - 970027
AU - Gumulec, Jaromír - Masařík, Michal - Křížková, Soňa - Hlavna, Marián - Babula, P. - Hrabec, Roman - Rovný, Arne - Masaříková, Miroslava - Sochor, J. - Adam, V. - Eckschlager, T. - Kizek, René
PY - 2012
TI - Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers
JF - Neoplasma
VL - 59
IS - 2
SP - 191
EP - 191
SN - 00282685
KW - cancer
KW - tumor marker
KW - immunodetection
KW - electrochemistry
KW - polymerase chain reaction
KW - mRNA
N2 - Current diagnostic techniques are inefficient in distinguishing latent and low-risk forms of prostate cancer from high-risk forms. The present study is focused on determination of putative tumor markers of aggressive high-grade forms of prostate cancer. Potential markers were determined in blood sera of 133 patients (82 cases and 51 controls) and in cell lines (Gleason score 9-derived 22Rv1 and normal tissue derived PNT1A) on mRNA and protein levels. Alpha-methylacyl-CoA racemase (AMACR), metallothionein classes 1A and 2A (MT1A and MT2A) were determined and compared to prostate specific antigen (PSA) levels. On mRNA level, significantly increased expression of MT2A (2.4-fold), PSA (2.6-fold) and AMACR (8.4-fold) and insignificantly (1.9-fold) elevated MT1A in 22Rv1 compared to non-tumor PNT1A were determined. On protein level, significant enhancement of free PSA and total PSA in tumor cell line was evident. AMACR protein was 1.5-fold elevated in tumor line (below the level of significance). Contrary to mRNA, significantly (p = 0.01) reduced level of MT protein in tumor lines was determined. In the case of serum level, significantly enhanced MT level (4.5-fold) in patients’ sera was found. No significant changes were observed in the case of AMACR. These findings indicate possible alternative role of MT to PSA prostate cancer marker. In addition, level of AMACR is distinctly higher in the Gleason score 9 in serum of patients and MT shows a descending trend in relation to Gleason score.
ER -
GUMULEC, Jaromír, Michal MASAŘÍK, Soňa KŘÍŽKOVÁ, Marián HLAVNA, P. BABULA, Roman HRABEC, Arne ROVNÝ, Miroslava MASAŘÍKOVÁ, J. SOCHOR, V. ADAM, T. ECKSCHLAGER a René KIZEK. Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers. \textit{Neoplasma}. 2012, roč.~59, č.~2, s.~191-200. ISSN~0028-2685. Dostupné z: https://dx.doi.org/10.4149/neo\_{}2012\_{}025.
|
