Modulation of death receptor-mediated apoptosis in
differentiating human myeloid leukemia HL-60 cells

J 2001

Modulation of death receptor-mediated apoptosis in differentiating human myeloid leukemia HL-60 cells

VONDRÁČEK, Jan; M.A. SHEARD; Pavel KREJČÍ; Kateřina MINKSOVÁ; Jiřina HOFMANOVÁ et al.

Základní údaje

Originální název

Modulation of death receptor-mediated apoptosis in differentiating human myeloid leukemia HL-60 cells

Autoři

VONDRÁČEK, Jan; M.A. SHEARD; Pavel KREJČÍ; Kateřina MINKSOVÁ; Jiřina HOFMANOVÁ a Alois KOZUBÍK

Vydání

Journal of Leukocyte Biology, New York, Society for Leukocyte Biology, 2001, 0741-5400

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.516

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000168806800016

Klíčová slova anglicky

all-trans retinoic acid; dimethyl sulfoxide; Fas/CD95/APO-1; TNF-alpha; Bcl-2; FAP-1

Změněno: 15. 2. 2012 16:50, Mgr. Jiřina Medalová, Ph.D.

Anotace

V originále

Differentiating myeloid cells may become resistant to various apoptotic stimuli. In the present study, dimethyl sulfoxide (DMSO) and all-trans retinoic acid (ATRA) were found to modulate the sensitivity of HL-60 cells to death receptor-mediated apoptosis in a time-dependent manner. During the early stages of differentiation, DMSO treatment increased the response of HL-60 cells to tumor necrosis factor cw; (TNF-alpha), but enhanced responsiveness was lost during later differentiation stages. In contrast, ATRA treatment induced resistance to TNF-alpha -induced apoptosis, HL-60 cells were resistant to Fas-mediated apoptosis but were sensitized by culturing in serum-free conditions. Similar to its effect on TNF-cr sensitivity, DMSO pretreatment augmented the response to Fas-mediated signaling, which coincided with increased expression of Fas on DMSO-pretreated cells. However, during the later stages of DMSO-induced differentiation, sensitivity to anti-Fas antibody-induced apoptosis declined significantly, although Fas expression was still elevated, The reduced sensitivity to anti-Fas treatment partially correlated with increased Fas-associated phosphatase-l mRNA expression, Thus, regardless of either Fas up-regulation or potentiation of TNF-alpha -mediated apoptosis during early DMSO-induced differentiation, a slow increase in resistance to apoptosis mediated through these death receptors occurs during DMSO-induced differentiation, which contrasts with the rapid induction of resistance following treatment with ATRA.

Citovat

VONDRÁČEK, Jan; M.A. SHEARD; Pavel KREJČÍ; Kateřina MINKSOVÁ; Jiřina HOFMANOVÁ a Alois KOZUBÍK. Modulation of death receptor-mediated apoptosis in differentiating human myeloid leukemia HL-60 cells. Journal of Leukocyte Biology. New York: Society for Leukocyte Biology, 2001, roč. 69, č. 5, s. 794-802. ISSN 0741-5400.

@article{971690,
   author = {Vondráček, Jan and Sheard, M.A. and Krejčí, Pavel and Minksová, Kateřina and Hofmanová, Jiřina and Kozubík, Alois},
   article_location = {New York},
   article_number = {5},
   keywords = {all-trans retinoic acid; dimethyl sulfoxide; Fas/CD95/APO-1; TNF-alpha; Bcl-2; FAP-1},
   language = {eng},
   issn = {0741-5400},
   journal = {Journal of Leukocyte Biology},
   title = {Modulation of death receptor-mediated apoptosis in differentiating human myeloid leukemia HL-60 cells},
   volume = {69},
   year = {2001}
}

TY  - JOUR
ID  - 971690
AU  - Vondráček, Jan - Sheard, M.A. - Krejčí, Pavel - Minksová, Kateřina - Hofmanová, Jiřina - Kozubík, Alois
PY  - 2001
TI  - Modulation of death receptor-mediated apoptosis in differentiating human myeloid leukemia HL-60 cells
JF  - Journal of Leukocyte Biology
VL  - 69
IS  - 5
SP  - 794-802
EP  - 794-802
PB  - Society for Leukocyte Biology
SN  - 07415400
KW  - all-trans retinoic acid
KW  - dimethyl sulfoxide
KW  - Fas/CD95/APO-1
KW  - TNF-alpha
KW  - Bcl-2
KW  - FAP-1
N2  - Differentiating myeloid cells may become resistant to various apoptotic stimuli. In the present study, dimethyl sulfoxide (DMSO) and all-trans retinoic acid (ATRA) were found to modulate the sensitivity of HL-60 cells to death receptor-mediated apoptosis in a time-dependent manner. During the early stages of differentiation, DMSO treatment increased the response of HL-60 cells to tumor necrosis factor cw; (TNF-alpha), but enhanced responsiveness was lost during later differentiation stages. In contrast, ATRA treatment induced resistance to TNF-alpha -induced apoptosis, HL-60 cells were resistant to Fas-mediated apoptosis but were sensitized by culturing in serum-free conditions. Similar to its effect on TNF-cr sensitivity, DMSO pretreatment augmented the response to Fas-mediated signaling, which coincided with increased expression of Fas on DMSO-pretreated cells. However, during the later stages of DMSO-induced differentiation, sensitivity to anti-Fas antibody-induced apoptosis declined significantly, although Fas expression was still elevated, The reduced sensitivity to anti-Fas treatment partially correlated with increased Fas-associated phosphatase-l mRNA expression, Thus, regardless of either Fas up-regulation or potentiation of TNF-alpha -mediated apoptosis during early DMSO-induced differentiation, a slow increase in resistance to apoptosis mediated through these death receptors occurs during DMSO-induced differentiation, which contrasts with the rapid induction of resistance following treatment with ATRA.
ER  -

VONDRÁČEK, Jan; M.A. SHEARD; Pavel KREJČÍ; Kateřina MINKSOVÁ; Jiřina HOFMANOVÁ a Alois KOZUBÍK. Modulation of death receptor-mediated apoptosis in differentiating human myeloid leukemia HL-60 cells. \textit{Journal of Leukocyte Biology}. New York: Society for Leukocyte Biology, 2001, roč.~69, č.~5, s.~794-802. ISSN~0741-5400.

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