2012
Determination of Lead in Submicroliter Samples of Whole Blood using Laser Diode Thermal Vaporization
PREISLER, Jan; Pavla FOLTYNOVÁ a Viktor KANICKÝZákladní údaje
Originální název
Determination of Lead in Submicroliter Samples of Whole Blood using Laser Diode Thermal Vaporization
Autoři
Vydání
27th International Symposium on MicroScale Bioseparations and Analyses, MSB 2012, 2012
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10406 Analytical chemistry
Stát vydavatele
Švýcarsko
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14740/12:00057267
Organizační jednotka
Středoevropský technologický institut
Klíčová slova anglicky
diode laser; inductively-coupled plasma; mass spectrometry; sample introduction; ICP MS
Štítky
Změněno: 15. 4. 2013 14:37, Mgr. Nikola Kostlánová, Ph.D.
Anotace
V originále
A new method for determination of trace elements in submicroliter sample volumes, laser diode thermal vaporization (LDTV) inductively-coupled plasma mass spectrometry (ICP-MS) is presented. Submicroliter samples for LDTV ICP-MS are deposited onto a suitable substrate sheet containing an absorber. After drying, samples are desorbed from the substrate in a commercial laser ablation chamber equipped with a near infrared continuous-wave diode laser and analyzed with an ICP quadrupole mass spectrometer. The laser induces pyrolysis and/or burning of the substrate and the generated aerosol is carried out into the plasma torch. Selection of experimental arrangement and laser wavelength as well as optimization of experimental conditions, e.g., raster type, scan speed, additives etc. was examined. An arrangement employing common paper as the substrate, commercial printer black ink as the absorber and 808 nm diode laser was chosen. The limits of detection of Co, Ni, Zn, Mo, Cd, Sn and Pb deposited on the pre-printed paper were found to be in a low pg range. The technique was applied to determination of lead in whole blood without any sample treatment. Other applications included analysis of Co in a drug preparation and tin in canned food. Potential coupling with planar and microcolumn separations will be discussed. In summary, LDTV ICP-MS is suitable for quantitative elemental analysis of low volume samples (typically 100 – 500 nL). The method does not require an expensive high-energy pulse laser, which makes it a promising alternative to nebulizers. Other advantages are also easy archiving, transportation, option of prearranged multi-elemental calibration sets and high throughput due to minimization of memory effects.
Návaznosti
| ED1.1.00/02.0068, projekt VaV |
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| GAP206/12/0538, projekt VaV |
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| GA203/09/1025, projekt VaV |
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| MSM0021622411, záměr |
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| MSM0021622412, záměr |
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| MSM0021622415, záměr |
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