Commonly used ROS-sensitive fluorescent probes are substrates
of ABC transporters

a 2011

Commonly used ROS-sensitive fluorescent probes are substrates of ABC transporters

PROCHÁZKOVÁ, Jiřina; Lukáš KUBALA; Hana KOTASOVÁ; Iva GUDERNOVÁ; Zuzana ŠRÁMKOVÁ et al.

Základní údaje

Originální název

Commonly used ROS-sensitive fluorescent probes are substrates of ABC transporters

Autoři

PROCHÁZKOVÁ, Jiřina; Lukáš KUBALA; Hana KOTASOVÁ; Iva GUDERNOVÁ; Zuzana ŠRÁMKOVÁ; Michaela PEKAROVÁ; Balazs SARKADI a Jiří PACHERNÍK

Vydání

VI. konference Analytická cytometri, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/11:00050554

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-260-0676-3

Klíčová slova anglicky

ABC transporters; mitochondria; fluorescent probe

Změněno: 20. 3. 2012 14:47, Mgr. Jiřina Medalová, Ph.D.

Anotace

V originále

Intracellular ROS production significantly regulates the fate of various cell types through redox interaction with signal transduction pathways (reviewed in Hensley et al. 2000). Thus, the determination of the actual intracellular ROS level in cells is crucial for understanding the ROS role in the key processes governing differentiation, cellular death and carcinogenesis. Intracellular ROS are usually produced by non-phagocytic NADPH oxidases and mitochondria (Kowaltowski et al. 2009). Thus, mitochondria mass and membrane potential are often determined together with intracellular ROS production. A wide spectrum of fluorescent redox-sensitive probes for the determination of intracellular ROS production have been developed and employed in numerous studies (Soh 2006, Dickinson et al. 2009). Both the high sensitivity and the reproducibility of results of are directly connected with the preservation of the oxidized/reduced fluorescent probe inside the cell.

Návaznosti

GA301/08/0717, projekt VaV

Název: Regulace diferenciace ES buněk prostřednictvím receptoru gp130

Investor: Grantová agentura ČR, Regulace diferenciace ES buněk prostřednictvím receptoru gp130

MUNI/A/0975/2009, interní kód MU

Název: Podpora výzkumné činnosti studentů v oblasti fyziologie a imunologie živočichů. (Akronym: VYFIŽ)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů v oblasti fyziologie a imunologie živočichů., DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

PROCHÁZKOVÁ, Jiřina; Lukáš KUBALA; Hana KOTASOVÁ; Iva GUDERNOVÁ; Zuzana ŠRÁMKOVÁ; Michaela PEKAROVÁ; Balazs SARKADI a Jiří PACHERNÍK. Commonly used ROS-sensitive fluorescent probes are substrates of ABC transporters. In VI. konference Analytická cytometri. 2011. ISBN 978-80-260-0676-3.

@proceedings{974560,
   author = {Procházková, Jiřina and Kubala, Lukáš and Kotasová, Hana and Gudernová, Iva and Šrámková, Zuzana and Pekarová, Michaela and Sarkadi, Balazs and Pacherník, Jiří},
   booktitle = {VI. konference Analytická cytometri},
   keywords = {ABC transporters; mitochondria; fluorescent probe},
   language = {eng},
   isbn = {978-80-260-0676-3},
   title = {Commonly used ROS-sensitive fluorescent probes are substrates of ABC transporters},
   year = {2011}
}

TY  - CONF
ID  - 974560
AU  - Procházková, Jiřina - Kubala, Lukáš - Kotasová, Hana - Gudernová, Iva - Šrámková, Zuzana - Pekarová, Michaela - Sarkadi, Balazs - Pacherník, Jiří
PY  - 2011
TI  - Commonly used ROS-sensitive fluorescent probes are substrates of ABC transporters
SN  - 9788026006763
KW  - ABC transporters
KW  - mitochondria
KW  - fluorescent probe
N2  - Intracellular ROS production significantly regulates the fate of various cell types through redox interaction with signal transduction pathways (reviewed in Hensley et al. 2000). Thus, the determination of the actual intracellular ROS level in cells is crucial for understanding the ROS role in the key processes governing differentiation, cellular death and carcinogenesis. Intracellular ROS are usually produced by non-phagocytic NADPH oxidases and mitochondria (Kowaltowski et al. 2009). Thus, mitochondria mass and membrane potential are often determined together with intracellular ROS production. A wide spectrum of fluorescent redox-sensitive probes for the determination of intracellular ROS production have been developed and employed in numerous studies (Soh 2006, Dickinson et al. 2009). Both the high sensitivity and the reproducibility of results of are directly connected with the preservation of the oxidized/reduced fluorescent probe inside the cell.
ER  -

PROCHÁZKOVÁ, Jiřina; Lukáš KUBALA; Hana KOTASOVÁ; Iva GUDERNOVÁ; Zuzana ŠRÁMKOVÁ; Michaela PEKAROVÁ; Balazs SARKADI a Jiří PACHERNÍK. Commonly used ROS-sensitive fluorescent probes are substrates of ABC transporters. In \textit{VI. konference Analytická cytometri}. 2011. ISBN~978-80-260-0676-3.

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