Trajectories and nuclear arrangement of PML bodies are
influenced by A-type lamin deficiency

J 2012

Trajectories and nuclear arrangement of PML bodies are influenced by A-type lamin deficiency

STIXOVÁ, Lenka; Pavel MATULA; Stanislav KOZUBEK; Dušan CMARKO; Adriana GOMBITOVÁ et al.

Základní údaje

Originální název

Trajectories and nuclear arrangement of PML bodies are influenced by A-type lamin deficiency

Autoři

STIXOVÁ, Lenka; Pavel MATULA; Stanislav KOZUBEK; Dušan CMARKO; Adriana GOMBITOVÁ; Ivan RAŠKA a Eva BÁRTOVÁ

Vydání

Biology of the Cell, 2012, 0248-4900

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.488

Kód RIV

RIV/00216224:14330/12:00057299

Organizační jednotka

Fakulta informatiky

DOI

https://doi.org/10.1111/boc.201100053

UT WoS

000305990900006

Klíčová slova anglicky

ACUTE PROMYELOCYTIC LEUKEMIA; DOUBLE-STRAND BREAKS; HUMAN-CELLS; PROTEIN STRUCTURES; STEM-CELLS; RAR-ALPHA; RNA; TELOMERE; HETEROCHROMATIN; TRANSCRIPTION

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 4. 2013 00:18, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

Background information Promyelocytic leukaemia (PML) bodies are specific nuclear structures with functional significance for acute promyelocytic leukaemia. In this study, we analysed the trajectories of PML bodies using single-particle tracking. Results We observed that the recovery of PML protein after photobleaching was ATP dependent in both wild-type (wt) and A-type lamin-deficient cells. The movement of PML bodies was faster and the nuclear area occupied by particular PML bodies was larger in A-type lamin-deficient fibroblasts compared with their wt counterparts. Moreover, dysfunction of the LMNA gene increased the frequency of mutual interactions between individual PML bodies and influenced the morphology of these domains at the ultrastructural level. As a consequence of A-type lamin deficiency, PML protein accumulated in nuclear blebs and frequently appeared at the nuclear periphery. Conclusions We suggest that the physiological function of lamin A proteins is important for events that occur in the compartment of PML bodies. This observation was confirmed in other experimental models characterised by lamin changes, including apoptosis or the differentiation of mouse embryonic stem cells.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

STIXOVÁ, Lenka; Pavel MATULA; Stanislav KOZUBEK; Dušan CMARKO; Adriana GOMBITOVÁ; Ivan RAŠKA a Eva BÁRTOVÁ. Trajectories and nuclear arrangement of PML bodies are influenced by A-type lamin deficiency. Biology of the Cell. 2012, roč. 104, č. 7, s. 418-432. ISSN 0248-4900. Dostupné z: https://doi.org/10.1111/boc.201100053.

@article{975303,
   author = {Stixová, Lenka and Matula, Pavel and Kozubek, Stanislav and Cmarko, Dušan and Gombitová, Adriana and Raška, Ivan and Bártová, Eva},
   article_number = {7},
   doi = {https://doi.org/10.1111/boc.201100053},
   keywords = {ACUTE PROMYELOCYTIC LEUKEMIA; DOUBLE-STRAND BREAKS; HUMAN-CELLS; PROTEIN STRUCTURES; STEM-CELLS; RAR-ALPHA; RNA; TELOMERE; HETEROCHROMATIN; TRANSCRIPTION},
   language = {eng},
   issn = {0248-4900},
   journal = {Biology of the Cell},
   title = {Trajectories and nuclear arrangement of PML bodies are influenced by A-type lamin deficiency},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/boc.201100053/abstract},
   volume = {104},
   year = {2012}
}

TY  - JOUR
ID  - 975303
AU  - Stixová, Lenka - Matula, Pavel - Kozubek, Stanislav - Cmarko, Dušan - Gombitová, Adriana - Raška, Ivan - Bártová, Eva
PY  - 2012
TI  - Trajectories and nuclear arrangement of PML bodies are influenced by A-type lamin deficiency
JF  - Biology of the Cell
VL  - 104
IS  - 7
SP  - 418-432
EP  - 418-432
SN  - 02484900
KW  - ACUTE PROMYELOCYTIC LEUKEMIA
KW  - DOUBLE-STRAND BREAKS
KW  - HUMAN-CELLS
KW  - PROTEIN STRUCTURES
KW  - STEM-CELLS
KW  - RAR-ALPHA
KW  - RNA
KW  - TELOMERE
KW  - HETEROCHROMATIN
KW  - TRANSCRIPTION
UR  - http://onlinelibrary.wiley.com/doi/10.1111/boc.201100053/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1111/boc.201100053/abstract
N2  - Background information Promyelocytic leukaemia (PML) bodies are specific nuclear structures with functional significance for acute promyelocytic leukaemia. In this study, we analysed the trajectories of PML bodies using single-particle tracking. Results We observed that the recovery of PML protein after photobleaching was ATP dependent in both wild-type (wt) and A-type lamin-deficient cells. The movement of PML bodies was faster and the nuclear area occupied by particular PML bodies was larger in A-type lamin-deficient fibroblasts compared with their wt counterparts. Moreover, dysfunction of the LMNA gene increased the frequency of mutual interactions between individual PML bodies and influenced the morphology of these domains at the ultrastructural level. As a consequence of A-type lamin deficiency, PML protein accumulated in nuclear blebs and frequently appeared at the nuclear periphery. Conclusions We suggest that the physiological function of lamin A proteins is important for events that occur in the compartment of PML bodies. This observation was confirmed in other experimental models characterised by lamin changes, including apoptosis or the differentiation of mouse embryonic stem cells.
ER  -

STIXOVÁ, Lenka; Pavel MATULA; Stanislav KOZUBEK; Dušan CMARKO; Adriana GOMBITOVÁ; Ivan RAŠKA a Eva BÁRTOVÁ. Trajectories and nuclear arrangement of PML bodies are influenced by A-type lamin deficiency. \textit{Biology of the Cell}. 2012, roč.~104, č.~7, s.~418-432. ISSN~0248-4900. Dostupné z: https://doi.org/10.1111/boc.201100053.

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