2011
Study of ergosterol signal pathway
KAŠPAROVSKÝ, Tomáš, Jitka KLEMPOVÁ, Kateřina DADÁKOVÁ a Jan LOCHMANZákladní údaje
Originální název
Study of ergosterol signal pathway
Autoři
KAŠPAROVSKÝ, Tomáš (203 Česká republika, garant, domácí), Jitka KLEMPOVÁ (203 Česká republika, domácí), Kateřina DADÁKOVÁ (203 Česká republika, domácí) a Jan LOCHMAN (203 Česká republika, domácí)
Vydání
FEBS Journal, 2011
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 3.790
Kód RIV
RIV/00216224:14310/11:00050628
Organizační jednotka
Přírodovědecká fakulta
ISSN
UT WoS
000292333102209
Klíčová slova česky
ergosterol signální dráha
Klíčová slova anglicky
ergosterol signal pathway
Štítky
Změněno: 11. 4. 2012 12:25, prof. Mgr. Tomáš Kašparovský, Ph.D.
Anotace
V originále
Ergosterol principal compound of the fungal plasma membrane belongs to the group of elicitors called non-specific elicitors whose not differ in their effects on different cultivars within a plant species. It triggers a defence reaction in tobacco and tomato cells manifested by active oxygen species synthesis and changes of ion fluxes. Further production of the phytoalexin capsidiol and pathogenesis related proteins expression was observed. Even though some steps of signalling pathway including phospholipase A2 protein kinase C and intracellular calcium were proposed up today activation mechanism was not elucidate. But it is assumed role of some specific binding partner or lipid rafts. Here we have attempted to elucidate in more details of the signal pathway elicited by ergosterol. Therefore we focused on study of calcium signalling by using fluorescence confocal microscopy and specific inhibitors. Especially, we tested treatment of ergosterol on vacuoles in comparison with vacuole calcium release activators. Moreover, we tried to localise site of ergosterol recognition. We detected that elicitation by ergosterol increases the concentration of cytosolic calcium mainly from internal sources but direct activation of calcium release from vacuole by ergosterol didnt determined.
Návaznosti
GAP501/11/1003, projekt VaV |
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GP203/09/P248, projekt VaV |
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MSM0021622413, záměr |
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