Isolation of Quaternary Benzo[c] phenanthridine Alkaloids from
Macleaya microcarpa (MAXIM.) FEDDE: Comparison of Maceration,
Soxhlet Extraction and Pressurised Liquid Extraction

J 2012

Isolation of Quaternary Benzo[c] phenanthridine Alkaloids from Macleaya microcarpa (MAXIM.) FEDDE: Comparison of Maceration, Soxhlet Extraction and Pressurised Liquid Extraction

URBANOVÁ, Jana; Kristýna PĚNČÍKOVÁ; Jana GREGOROVÁ; Barbora HOHNOVÁ; Lenka ŠŤAVÍKOVÁ et al.

Základní údaje

Originální název

Isolation of Quaternary Benzo[c] phenanthridine Alkaloids from Macleaya microcarpa (MAXIM.) FEDDE: Comparison of Maceration, Soxhlet Extraction and Pressurised Liquid Extraction

Autoři

URBANOVÁ, Jana; Kristýna PĚNČÍKOVÁ; Jana GREGOROVÁ ; Barbora HOHNOVÁ; Lenka ŠŤAVÍKOVÁ; Pavel KARÁSEK; Michal ROTH a Eva TÁBORSKÁ

Vydání

Phytochemical Analysis, Hoboken, WILEY-BLACKWELL, 2012, 0958-0344

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.480

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/12:00057370

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Maceration; pressurised liquid extraction; Soxhlet extraction; benzo[c]phenanthridine alkaloids; Macleaya microcarpa

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 12. 2019 15:11, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Macleaya microcarpa (Papaveraceae family) has been of considerable interest in recent years as a prospective source of quaternary benzo[c]phenanthridine alkaloids (QBAs) related to many pharmaceutical beneficial effects. For this purpose, a quantitative, efficient and fast method to isolate the QBAs from the plant material is required. To optimise and compare pressurised liquid extraction (PLE) with Soxhlet extraction and maceration in order to estimate extraction conditions for fast and efficient isolation of QBAs contained in the roots of Macleaya microcarpa. The QBAs were extracted by PLE, Soxhlet extraction and maceration at different conditions (solvent, time, etc.). Reversed phase HPLC with diode-array detector was utilised for their determination and quantification. To optimise the PLE procedure, the variable parameters, including temperature (40C-150C), sample-to-inert material ratio, extraction time (5-30 min) and number of extraction cycles (1-4), were also tested. Quantitative determination of QBAs resulted in 0.2-2.8 mg/g, 0.3-2.5 mg/g and 0.3-3.1 mg/g for PLE, Soxhlet extraction and maceration. To produce the yields mentioned above, PLE required only up to 30 min compared with 21 h for Soxhlet extraction and 49 days for maceration. PLE provided an effective and fast extraction of QBAs fromM. microcarpa roots and can be recommended as an alternative isolation method to conventional techniques for QBAs from the plant sources.

Návaznosti

GA525/08/0819, projekt VaV

Název: Rostlinné zdroje minoritních benzofenanthridinových alkaloidů a studium interakcí těchto alkaloidů s DNA

Investor: Grantová agentura ČR, Rostlinné zdroje minoritních benzofenanthridinových alkaloidů a studium interakcí těchto alkaloidů s DNA

MUNI/A/0963/2010, interní kód MU

Název: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění

Investor: Masarykova univerzita, Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

URBANOVÁ, Jana; Kristýna PĚNČÍKOVÁ; Jana GREGOROVÁ; Barbora HOHNOVÁ; Lenka ŠŤAVÍKOVÁ; Pavel KARÁSEK; Michal ROTH a Eva TÁBORSKÁ. Isolation of Quaternary Benzo[c] phenanthridine Alkaloids from Macleaya microcarpa (MAXIM.) FEDDE: Comparison of Maceration, Soxhlet Extraction and Pressurised Liquid Extraction. Phytochemical Analysis. Hoboken: WILEY-BLACKWELL, 2012, roč. 23, č. 5, s. 477-482. ISSN 0958-0344. Dostupné z: https://doi.org/10.1002/pca.2344.

@article{980546,
   author = {Urbanová, Jana and Pěnčíková, Kristýna and Gregorová, Jana and Hohnová, Barbora and Šťavíková, Lenka and Karásek, Pavel and Roth, Michal and Táborská, Eva},
   article_location = {Hoboken},
   article_number = {5},
   doi = {https://doi.org/10.1002/pca.2344},
   keywords = {Maceration; pressurised liquid extraction; Soxhlet extraction; benzo[c]phenanthridine alkaloids; Macleaya microcarpa},
   language = {eng},
   issn = {0958-0344},
   journal = {Phytochemical Analysis},
   title = {Isolation of Quaternary Benzo[c] phenanthridine Alkaloids from Macleaya microcarpa (MAXIM.) FEDDE: Comparison of Maceration, Soxhlet Extraction and Pressurised Liquid Extraction},
   url = {https://onlinelibrary.wiley.com/doi/pdf/10.1002/pca.2344},
   volume = {23},
   year = {2012}
}

TY  - JOUR
ID  - 980546
AU  - Urbanová, Jana - Pěnčíková, Kristýna - Gregorová, Jana - Hohnová, Barbora - Šťavíková, Lenka - Karásek, Pavel - Roth, Michal - Táborská, Eva
PY  - 2012
TI  - Isolation of Quaternary Benzo[c] phenanthridine Alkaloids from Macleaya microcarpa (MAXIM.) FEDDE: Comparison of Maceration, Soxhlet Extraction and Pressurised Liquid Extraction
JF  - Phytochemical Analysis
VL  - 23
IS  - 5
SP  - 477-482
EP  - 477-482
PB  - WILEY-BLACKWELL
SN  - 09580344
KW  - Maceration
KW  - pressurised liquid extraction
KW  - Soxhlet extraction
KW  - benzo[c]phenanthridine alkaloids
KW  - Macleaya microcarpa
UR  - https://onlinelibrary.wiley.com/doi/pdf/10.1002/pca.2344
L2  - https://onlinelibrary.wiley.com/doi/pdf/10.1002/pca.2344
N2  - Macleaya microcarpa (Papaveraceae family) has been of considerable interest in recent years as a prospective source of quaternary benzo[c]phenanthridine alkaloids (QBAs) related to many pharmaceutical beneficial effects. For this purpose, a quantitative, efficient and fast method to isolate the QBAs from the plant material is required. To optimise and compare pressurised liquid extraction (PLE) with Soxhlet extraction and maceration in order to estimate extraction conditions for fast and efficient isolation of QBAs contained in the roots of Macleaya microcarpa. The QBAs were extracted by PLE, Soxhlet extraction and maceration at different conditions (solvent, time, etc.). Reversed phase HPLC with diode-array detector was utilised for their determination and quantification. To optimise the PLE procedure, the variable parameters, including temperature (40C-150C), sample-to-inert material ratio, extraction time (5-30 min) and number of extraction cycles (1-4), were also tested. Quantitative determination of QBAs resulted in 0.2-2.8 mg/g, 0.3-2.5 mg/g and 0.3-3.1 mg/g for PLE, Soxhlet extraction and maceration. To produce the yields mentioned above, PLE required only up to 30 min compared with 21 h for Soxhlet extraction and 49 days for maceration. PLE provided an effective and fast extraction of QBAs fromM. microcarpa roots and can be recommended as an alternative isolation method to conventional techniques for QBAs from the plant sources.
ER  -

URBANOVÁ, Jana; Kristýna PĚNČÍKOVÁ; Jana GREGOROVÁ; Barbora HOHNOVÁ; Lenka ŠŤAVÍKOVÁ; Pavel KARÁSEK; Michal ROTH a Eva TÁBORSKÁ. Isolation of Quaternary Benzo[c] phenanthridine Alkaloids from Macleaya microcarpa (MAXIM.) FEDDE: Comparison of Maceration, Soxhlet Extraction and Pressurised Liquid Extraction. \textit{Phytochemical Analysis}. Hoboken: WILEY-BLACKWELL, 2012, roč.~23, č.~5, s.~477-482. ISSN~0958-0344. Dostupné z: https://doi.org/10.1002/pca.2344.

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