Další formáty:
BibTeX
LaTeX
RIS
@article{981977,
author = {Tesařová, Lenka and Krontorád Koutná, Irena and Dvořáková, Magdaléna and Klabusay, Martin},
article_number = {5},
doi = {http://dx.doi.org/10.1111/j.1744-313X.2012.01114.x},
keywords = {Reverse transcription PCR; Capillary electrophoresis; gammadelta T cell; TCR; Vgamma and Vdelta gene families},
language = {eng},
issn = {1744-3121},
journal = {International Journal of immunogenetics},
title = {Relative expression of gammadelta T-cell receptor gene families detected by RT–PCR and capillary electrophoresis},
url = {http://onlinelibrary.wiley.com/doi/10.1111/j.1744-313X.2012.01114.x/full},
volume = {39},
year = {2012}
}
TY - JOUR
ID - 981977
AU - Tesařová, Lenka - Krontorád Koutná, Irena - Dvořáková, Magdaléna - Klabusay, Martin
PY - 2012
TI - Relative expression of gammadelta T-cell receptor gene families detected by RT–PCR and capillary electrophoresis
JF - International Journal of immunogenetics
VL - 39
IS - 5
SP - 381-388
EP - 381-388
PB - Blackwell Publishing Ltd
SN - 17443121
KW - Reverse transcription PCR
KW - Capillary electrophoresis
KW - gammadelta T cell
KW - TCR
KW - Vgamma and Vdelta gene families
UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1744-313X.2012.01114.x/full
L2 - http://onlinelibrary.wiley.com/doi/10.1111/j.1744-313X.2012.01114.x/full
N2 - Gammadelta T cells are intensively studied because their function in infection, allergy, autoimmune disease, cancer and post-transplant period is not yet fully understood. PCR-based techniques were established to study the gamma variable (Vgamma) and delta variable (Vdelta) gene families. PCR product evaluation is routinely carried out by Southern blot analysis or the third complementarity-determining region (CDR3) spectratyping, but a fast and simple assessment of Vgamma and Vdelta gene family expression is missing. The aim of our study was to test capillary electrophoresis as a potential method for evaluating the composition of the gammadelta T-cell population. This report provides optimized PCR conditions for gammadelta T-cell receptor amplification. Further it describes the utilization of capillary electrophoresis in the Agilent 2100 Bioanalyzer to evaluate the relative expression of Vgamma and Vdelta gene families after their amplification. An application of the methodology to peripheral blood mononuclear cell samples from patients during hemato-oncological treatment is shown. The described methodology is fast and simple to operate and is therefore suitable as a first screening of the gammadelta T-cell population composition in tissues of interest.
ER -
TESAŘOVÁ, Lenka, Irena KRONTORÁD KOUTNÁ, Magdaléna DVOŘÁKOVÁ a Martin KLABUSAY. Relative expression of gammadelta T-cell receptor gene families detected by RT–PCR and capillary electrophoresis. \textit{International Journal of immunogenetics}. Blackwell Publishing Ltd, roč.~39, č.~5, s.~381-388. ISSN~1744-3121. doi:10.1111/j.1744-313X.2012.01114.x. 2012.
|
