The effects of H1-antihistamines on the nitric oxide production
by RAW 264.7 cells with respect to their lipophilicity.

J 2009

The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity.

MORAVCOVÁ, Jana; L RACKOVÁ; Michaela PEKAROVÁ; Lukáš KUBALA; Radomír NOSÁL et al.

Základní údaje

Originální název

The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity.

Autoři

MORAVCOVÁ, Jana; L RACKOVÁ; Michaela PEKAROVÁ; Lukáš KUBALA; Radomír NOSÁL; Viera JANČINOVÁ; Milan ČÍŽ a Antonín LOJEK

Vydání

International Immunopharmacology, Amsterdam, Netherlands, Elsevier, 2009, 1567-5769

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.214

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.intimp.2009.04.005

UT WoS

000267444800026

Klíčová slova česky

H1-antihistamines; Nitric oxide; RAW 264.7 cells; Inducible nitric oxide synthase; Nitrites

Klíčová slova anglicky

H1-antihistamines; Nitric oxide; RAW 264.7 cells; Inducible nitric oxide synthase; Nitrites

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 5. 2012 20:55, Mgr. Michaela Pekarová, Ph.D.

Anotace

V originále

H1-antihistamines are known to be important modulators of inflammatory response. However, the information about the influence of these drugs on reactive nitrogen species generation is still controversial. The main aim of the present study was to investigate the effects of selected H1-antihistamines on nitric oxide production by lipopolysaccharide-stimulated murine macrophages RAW 264.7, measured as changes in inducible nitric oxide synthase (iNOS) protein expression in cell lysates by Western blotting and nitrite formation in cell supernatants using the Griess reaction. In pharmacological non-toxic concentrations, H1-antihistamines significantly inhibited nitrite accumulation that was not caused by the scavenging ability of drugs against nitric oxide, measured amperometrically. The degree of inhibition of nitrite accumulation positively correlated with the degree of tested lipophilicity, measured by reversed-phase thin layer chromatography. Furthermore, H1-antihistamines differentially modulated the iNOS protein expression. In conclusion, as was shown in this study, the modulation of nitric oxide production could be caused by the downregulation of iNOS protein expression and/or the iNOS protein activity.

Citovat

MORAVCOVÁ, Jana; L RACKOVÁ; Michaela PEKAROVÁ; Lukáš KUBALA; Radomír NOSÁL; Viera JANČINOVÁ; Milan ČÍŽ a Antonín LOJEK. The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity. International Immunopharmacology. Amsterdam, Netherlands: Elsevier, 2009, roč. 9, 7-8, s. 990-995, 5 s. ISSN 1567-5769. Dostupné z: https://doi.org/10.1016/j.intimp.2009.04.005.

@article{983197,
   author = {Moravcová, Jana and Racková, L and Pekarová, Michaela and Kubala, Lukáš and Nosál, Radomír and Jančinová, Viera and Číž, Milan and Lojek, Antonín},
   article_location = {Amsterdam, Netherlands},
   article_number = {7-8},
   doi = {https://doi.org/10.1016/j.intimp.2009.04.005},
   keywords = {H1-antihistamines; Nitric oxide; RAW 264.7 cells; Inducible nitric oxide synthase; Nitrites},
   issn = {1567-5769},
   journal = {International Immunopharmacology},
   title = {The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity.},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/19362170},
   volume = {9},
   year = {2009}
}

TY  - JOUR
ID  - 983197
AU  - Moravcová, Jana - Racková, L - Pekarová, Michaela - Kubala, Lukáš - Nosál, Radomír - Jančinová, Viera - Číž, Milan - Lojek, Antonín
PY  - 2009
TI  - The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity.
JF  - International Immunopharmacology
VL  - 9
IS  - 7-8
SP  - 990-995
EP  - 990-995
PB  - Elsevier
SN  - 15675769
KW  - H1-antihistamines
KW  - Nitric oxide
KW  - RAW 264.7 cells
KW  - Inducible nitric oxide synthase
KW  - Nitrites
UR  - http://www.ncbi.nlm.nih.gov/pubmed/19362170
N2  - H1-antihistamines are known to be important modulators of inflammatory response. However, the information about the influence of these drugs on reactive nitrogen species generation is still controversial. The main aim of the present study was to investigate the effects of selected H1-antihistamines on nitric oxide production by lipopolysaccharide-stimulated murine macrophages RAW 264.7, measured as changes in inducible nitric oxide synthase (iNOS) protein expression in cell lysates by Western blotting and nitrite formation in cell supernatants using the Griess reaction. In pharmacological non-toxic concentrations, H1-antihistamines significantly inhibited nitrite accumulation that was not caused by the scavenging ability of drugs against nitric oxide, measured amperometrically. The degree of inhibition of nitrite accumulation positively correlated with the degree of tested lipophilicity, measured by reversed-phase thin layer chromatography. Furthermore, H1-antihistamines differentially modulated the iNOS protein expression. In conclusion, as was shown in this study, the modulation of nitric oxide production could be caused by the downregulation of iNOS protein expression and/or the iNOS protein activity.
ER  -

MORAVCOVÁ, Jana; L RACKOVÁ; Michaela PEKAROVÁ; Lukáš KUBALA; Radomír NOSÁL; Viera JANČINOVÁ; Milan ČÍŽ a Antonín LOJEK. The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity. \textit{International Immunopharmacology}. Amsterdam, Netherlands: Elsevier, 2009, roč.~9, 7-8, s.~990-995, 5 s. ISSN~1567-5769. Dostupné z: https://doi.org/10.1016/j.intimp.2009.04.005.

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