Arrangement of nuclear structures is not transmitted through
mitosis but is identical in sister cells

J 2012

Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells

ORLOVA, Darya Yurevna, Lenka STIXOVÁ, Stanislav KOZUBEK, Hinco GIERMAN, Gabriela ŠUSTÁČKOVÁ et. al.

Základní údaje

Originální název

Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells

Autoři

ORLOVA, Darya Yurevna (643 Rusko), Lenka STIXOVÁ (203 Česká republika), Stanislav KOZUBEK (203 Česká republika), Hinco GIERMAN (840 Spojené státy), Gabriela ŠUSTÁČKOVÁ (203 Česká republika), Andrei CHERNYSHEV (643 Rusko), Ruslan MEDVEDEV (643 Rusko), Soňa LEGARTOVÁ (703 Slovensko), Rogier VERSTEEG (528 Nizozemské království), Pavel MATULA (203 Česká republika, garant, domácí), Roman STOKLASA (703 Slovensko, domácí) a Eva BÁRTOVÁ (203 Česká republika)

Vydání

Journal of Cellular Biochemistry, 2012, 0730-2312

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.062

Kód RIV

RIV/00216224:14330/12:00057413

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1002/jcb.24208

UT WoS

000308927200002

Klíčová slova česky

histones; chromatin; HP1 protein; photoconversion; Dendra2; Cajal bodies

Klíčová slova anglicky

histones; chromatin; HP1 protein; photoconversion; Dendra2; Cajal bodies

Štítky

best3

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 4. 2013 16:58, doc. RNDr. Pavel Matula, Ph.D.

Anotace

V originále

Although it is well known that chromosomes are non-randomly organized during interphase, it is not completely clear whether higher-order chromatin structure is transmitted from mother to daughter cells. Therefore, we addressed the question of how chromatin is rearranged during interphase and whether heterochromatin pattern is transmitted after mitosis. We additionally tested the similarity of chromatin arrangement in sister interphase nuclei. We noticed a very active cell rotation during interphase, especially when histone hyperacetylation was induced or transcription was inhibited. This natural phenomenon can influence the analysis of nuclear arrangement. Using photoconversion of Dendra2-tagged core histone H4 we showed that the distribution of chromatin in daughter interphase nuclei differed from that in mother cells. Similarly, the nuclear distribution of heterochromatin protein 1beta (HP1beta) was not completely identical in mother and daughter cells. However, identity between mother and daughter cells was in many cases evidenced by nucleolar composition. Moreover, morphology of nucleoli, HP1beta protein, Cajal bodies, chromosome territories, and gene transcripts were identical in sister cell nuclei. We conclude that the arrangement of interphase chromatin is not transmitted through mitosis, but the chromatin pattern is identical in naturally synchronized sister cells. It is also necessary to take into account the possibility that cell rotation and the degree of chromatin condensation during functionally specific cell cycle phases might influence our view of nuclear architecture.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

MUNI/A/0914/2009, interní kód MU

Název: Rozsáhlé výpočetní systémy: modely, aplikace a verifikace (Akronym: SV-FI MAV)

Investor: Masarykova univerzita, Rozsáhlé výpočetní systémy: modely, aplikace a verifikace, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

ORLOVA, Darya Yurevna, Lenka STIXOVÁ, Stanislav KOZUBEK, Hinco GIERMAN, Gabriela ŠUSTÁČKOVÁ, Andrei CHERNYSHEV, Ruslan MEDVEDEV, Soňa LEGARTOVÁ, Rogier VERSTEEG, Pavel MATULA, Roman STOKLASA a Eva BÁRTOVÁ. Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells. Journal of Cellular Biochemistry. 2012, roč. 113, č. 11, s. 3313-3329. ISSN 0730-2312. Dostupné z: https://dx.doi.org/10.1002/jcb.24208.

@article{983372,
   author = {Orlova, Darya Yurevna and Stixová, Lenka and Kozubek, Stanislav and Gierman, Hinco and Šustáčková, Gabriela and Chernyshev, Andrei and Medvedev, Ruslan and Legartová, Soňa and Versteeg, Rogier and Matula, Pavel and Stoklasa, Roman and Bártová, Eva},
   article_number = {11},
   doi = {http://dx.doi.org/10.1002/jcb.24208},
   keywords = {histones; chromatin; HP1 protein; photoconversion; Dendra2; Cajal bodies},
   language = {eng},
   issn = {0730-2312},
   journal = {Journal of Cellular Biochemistry},
   title = {Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract},
   volume = {113},
   year = {2012}
}

TY  - JOUR
ID  - 983372
AU  - Orlova, Darya Yurevna - Stixová, Lenka - Kozubek, Stanislav - Gierman, Hinco - Šustáčková, Gabriela - Chernyshev, Andrei - Medvedev, Ruslan - Legartová, Soňa - Versteeg, Rogier - Matula, Pavel - Stoklasa, Roman - Bártová, Eva
PY  - 2012
TI  - Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells
JF  - Journal of Cellular Biochemistry
VL  - 113
IS  - 11
SP  - 3313-3329
EP  - 3313-3329
SN  - 07302312
KW  - histones
KW  - chromatin
KW  - HP1 protein
KW  - photoconversion
KW  - Dendra2
KW  - Cajal bodies
UR  - http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract
N2  - Although it is well known that chromosomes are non-randomly organized during interphase, it is not completely clear whether higher-order chromatin structure is transmitted from mother to daughter cells. Therefore, we addressed the question of how chromatin is rearranged during interphase and whether heterochromatin pattern is transmitted after mitosis. We additionally tested the similarity of chromatin arrangement in sister interphase nuclei. We noticed a very active cell rotation during interphase, especially when histone hyperacetylation was induced or transcription was inhibited. This natural phenomenon can influence the analysis of nuclear arrangement. Using photoconversion of Dendra2-tagged core histone H4 we showed that the distribution of chromatin in daughter interphase nuclei differed from that in mother cells. Similarly, the nuclear distribution of heterochromatin protein 1beta (HP1beta) was not completely identical in mother and daughter cells. However, identity between mother and daughter cells was in many cases evidenced by nucleolar composition. Moreover, morphology of nucleoli, HP1beta protein, Cajal bodies, chromosome territories, and gene transcripts were identical in sister cell nuclei. We conclude that the arrangement of interphase chromatin is not transmitted through mitosis, but the chromatin pattern is identical in naturally synchronized sister cells. It is also necessary to take into account the possibility that cell rotation and the degree of chromatin condensation during functionally specific cell cycle phases might influence our view of nuclear architecture.
ER  -

ORLOVA, Darya Yurevna, Lenka STIXOVÁ, Stanislav KOZUBEK, Hinco GIERMAN, Gabriela ŠUSTÁČKOVÁ, Andrei CHERNYSHEV, Ruslan MEDVEDEV, Soňa LEGARTOVÁ, Rogier VERSTEEG, Pavel MATULA, Roman STOKLASA a Eva BÁRTOVÁ. Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells. \textit{Journal of Cellular Biochemistry}. 2012, roč.~113, č.~11, s.~3313-3329. ISSN~0730-2312. Dostupné z: https://dx.doi.org/10.1002/jcb.24208.

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