SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES

D 2012

SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES

PILAŘOVÁ, Iveta, Gabriela CASTILLO and Libuše TRNKOVÁ

Basic information

Original name

SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES

Authors

PILAŘOVÁ, Iveta (203 Czech Republic, belonging to the institution), Gabriela CASTILLO (218 Ecuador) and Libuše TRNKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Brno, XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků. p. 216-219, 4 pp. 2012

Publisher

Mendelova univerzita v Brně

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10403 Physical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

RIV identification code

RIV/00216224:14740/12:00057434

Organization unit

Central European Institute of Technology

ISBN

978-80-7375-618-5

Keywords in English

Gold nanoparticles;GNPs;UV-Vis spectroscopy;PCS;thrombin;aptamer

Abstract

V originále

Gold nanoparticles (GNPs) are the object of interest in the field of nanotechnology due to their unique electronic, structural, optical, magnetic, thermal, and catalytic properties. They are extensively applied in the construction of electrochemical sensors and biosensors. The aim of this contribution is to characterize GNPs of both sizes (diameters of 5nm and 10 nm) in buffered and non-buffered aqueous solutions with the change of ionic strength by spectroscopic methods (UV/ Vis, photon correlation spectroscopy), and to investigate the GNP–aptamer–thrombin system as an application of GNPs in a sensitive aptasensor. For the immobilization the thrombin-binding aptamer (TBA) with the sequence GGT TGG TGT GGT TGG on the GNP surface was modified by a sulfhydryl group. The study showed that the spectral characterization of GNPs depends strongly not only on the aptamer–thrombin conjugate but also on the GNP size and solution composition (buffer, ions and their concentrations).

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GD106/09/H035, research and development project

Name: Víceúrovňový design pokrokových materiálů

Investor: Czech Science Foundation

Citovat

PILAŘOVÁ, Iveta, Gabriela CASTILLO and Libuše TRNKOVÁ. SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES. In XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků. Brno: Mendelova univerzita v Brně, 2012, p. 216-219. ISBN 978-80-7375-618-5.

@inproceedings{984223,
   author = {Pilařová, Iveta and Castillo, Gabriela and Trnková, Libuše},
   address = {Brno},
   booktitle = {XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků.},
   keywords = {Gold nanoparticles;GNPs;UV-Vis spectroscopy;PCS;thrombin;aptamer},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-7375-618-5},
   pages = {216-219},
   publisher = {Mendelova univerzita v Brně},
   title = {SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES},
   year = {2012}
}

TY  - JOUR
ID  - 984223
AU  - Pilařová, Iveta - Castillo, Gabriela - Trnková, Libuše
PY  - 2012
TI  - SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES
PB  - Mendelova univerzita v Brně
CY  - Brno
SN  - 9788073756185
KW  - Gold nanoparticles;GNPs;UV-Vis spectroscopy;PCS;thrombin;aptamer
N2  - Gold nanoparticles (GNPs) are the object of interest in the field of nanotechnology due to their unique electronic, structural, optical, magnetic, thermal, and catalytic properties. They are extensively applied in the construction of electrochemical sensors and biosensors. The aim of this contribution is to characterize GNPs of both sizes (diameters of 5nm and 10 nm) in buffered and non-buffered aqueous solutions with the change of ionic strength by spectroscopic methods (UV/ Vis, photon correlation spectroscopy), and to investigate the GNP–aptamer–thrombin system as an application of GNPs in a sensitive aptasensor. For the immobilization the thrombin-binding aptamer (TBA) with the sequence GGT TGG TGT GGT TGG on the GNP surface was modified by a sulfhydryl group. The study showed that the spectral characterization of GNPs depends strongly not only on the aptamer–thrombin conjugate but also on the GNP size and solution composition (buffer, ions and their concentrations).
ER  -

PILAŘOVÁ, Iveta, Gabriela CASTILLO and Libuše TRNKOVÁ. SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES. In \textit{XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků.}. Brno: Mendelova univerzita v Brně, 2012, p.~216-219. ISBN~978-80-7375-618-5.

Detailed Information on Publication Record