Sequential modifications in class II transactivator isoform 1
induced by lipopolysaccharide stimulate major
histocompatibility complex class II transcription in
macrophages

J 2006

Sequential modifications in class II transactivator isoform 1 induced by lipopolysaccharide stimulate major histocompatibility complex class II transcription in macrophages

DROZINA, Gorazd, Jiří KOHOUTEK, Tadashi NISHIYA and B Matija PETERLIN

Basic information

Original name

Sequential modifications in class II transactivator isoform 1 induced by lipopolysaccharide stimulate major histocompatibility complex class II transcription in macrophages

Authors

DROZINA, Gorazd, Jiří KOHOUTEK, Tadashi NISHIYA and B Matija PETERLIN

Edition

Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2006, 0021-9258

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.808

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1074/jbc.M608538200

UT WoS

000243033900024

Keywords in English

ELONGATION-FACTOR-B; UBIQUITIN-MEDIATED PROTEOLYSIS; HUMAN-IMMUNODEFICIENCY-VIRUS; CREB BINDING-PROTEIN; TOLL-LIKE RECEPTORS; ACTIVATE TRANSCRIPTION; DIFFERENTIAL USAGE; DENDRITIC CELLS; CIITA FUNCTION; IFN-GAMMA

Abstract

V originále

By presenting antigenic peptides on major histocompatibility complex class (MHC) II determinants to CD4(+) T cells, macrophages help to direct the establishment of adaptive immunity. We found that in these cells, lipopolysaccharide stimulates the expression of MHC II genes via the activation of Erk1/2, which is mediated by Toll-like receptor 4. Erk1/2 then phosphorylates the serine at position 357, which is located in a degron of CIITA isoform 1 that leads to its monoubiquitylation. Thus modified, CIITA isoform 1 binds P-TEFb, which mediates the elongation of RNA polymerase II and co-transcriptional processing of nascent transcripts. This induction leads to the expression of MHC II genes. Subsequent polyubiquitylation results in the degradation of CIITA isoform 1. Thus, the signaling cascade from Toll-like receptor 4 to CIITA isoform 1 represents one connection between innate and adaptive immunity in macrophages.

Citovat

DROZINA, Gorazd, Jiří KOHOUTEK, Tadashi NISHIYA and B Matija PETERLIN. Sequential modifications in class II transactivator isoform 1 induced by lipopolysaccharide stimulate major histocompatibility complex class II transcription in macrophages. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2006, vol. 281, No 52, p. 39963-39970. ISSN 0021-9258. Available from: https://dx.doi.org/10.1074/jbc.M608538200.

@article{987487,
   author = {Drozina, Gorazd and Kohoutek, Jiří and Nishiya, Tadashi and Peterlin, B Matija},
   article_location = {Bethesda, USA},
   article_number = {52},
   doi = {http://dx.doi.org/10.1074/jbc.M608538200},
   keywords = {ELONGATION-FACTOR-B; UBIQUITIN-MEDIATED PROTEOLYSIS; HUMAN-IMMUNODEFICIENCY-VIRUS; CREB BINDING-PROTEIN; TOLL-LIKE RECEPTORS; ACTIVATE TRANSCRIPTION; DIFFERENTIAL USAGE; DENDRITIC CELLS; CIITA FUNCTION; IFN-GAMMA},
   language = {eng},
   issn = {0021-9258},
   journal = {Journal of Biological Chemistry},
   title = {Sequential modifications in class II transactivator isoform 1 induced by lipopolysaccharide stimulate major histocompatibility complex class II transcription in macrophages},
   url = {http://www.jbc.org/content/early/2006/11/09/jbc.M608538200.full.pdf+html},
   volume = {281},
   year = {2006}
}

TY  - JOUR
ID  - 987487
AU  - Drozina, Gorazd - Kohoutek, Jiří - Nishiya, Tadashi - Peterlin, B Matija
PY  - 2006
TI  - Sequential modifications in class II transactivator isoform 1 induced by lipopolysaccharide stimulate major histocompatibility complex class II transcription in macrophages
JF  - Journal of Biological Chemistry
VL  - 281
IS  - 52
SP  - 39963-39970
EP  - 39963-39970
PB  - Amer. Soc. Biochem. Mol. Biol.
SN  - 00219258
KW  - ELONGATION-FACTOR-B
KW  - UBIQUITIN-MEDIATED PROTEOLYSIS
KW  - HUMAN-IMMUNODEFICIENCY-VIRUS
KW  - CREB BINDING-PROTEIN
KW  - TOLL-LIKE RECEPTORS
KW  - ACTIVATE TRANSCRIPTION
KW  - DIFFERENTIAL USAGE
KW  - DENDRITIC CELLS
KW  - CIITA FUNCTION
KW  - IFN-GAMMA
UR  - http://www.jbc.org/content/early/2006/11/09/jbc.M608538200.full.pdf+html
N2  - By presenting antigenic peptides on major histocompatibility complex class (MHC) II determinants to CD4(+) T cells, macrophages help to direct the establishment of adaptive immunity. We found that in these cells, lipopolysaccharide stimulates the expression of MHC II genes via the activation of Erk1/2, which is mediated by Toll-like receptor 4. Erk1/2 then phosphorylates the serine at position 357, which is located in a degron of CIITA isoform 1 that leads to its monoubiquitylation. Thus modified, CIITA isoform 1 binds P-TEFb, which mediates the elongation of RNA polymerase II and co-transcriptional processing of nascent transcripts. This induction leads to the expression of MHC II genes. Subsequent polyubiquitylation results in the degradation of CIITA isoform 1. Thus, the signaling cascade from Toll-like receptor 4 to CIITA isoform 1 represents one connection between innate and adaptive immunity in macrophages.
ER  -

DROZINA, Gorazd, Jiří KOHOUTEK, Tadashi NISHIYA and B Matija PETERLIN. Sequential modifications in class II transactivator isoform 1 induced by lipopolysaccharide stimulate major histocompatibility complex class II transcription in macrophages. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2006, vol.~281, No~52, p.~39963-39970. ISSN~0021-9258. Available from: https://dx.doi.org/10.1074/jbc.M608538200.

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