Cyclin T2 Is Essential for Mouse Embryogenesis

J 2009

Cyclin T2 Is Essential for Mouse Embryogenesis

KOHOUTEK, Jiří, Qintong LI, Dalibor BLAŽEK, Zeping LUO, Huimin JIANG et. al.

Základní údaje

Originální název

Cyclin T2 Is Essential for Mouse Embryogenesis

Autoři

KOHOUTEK, Jiří, Qintong LI, Dalibor BLAŽEK, Zeping LUO, Huimin JIANG a B Matija PETERLIN

Vydání

Molecular and Cellular Biology, Washington, D.C. ASM, 2009, 0270-7306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.057

DOI

http://dx.doi.org/10.1128/MCB.00172-09

UT WoS

000266436300004

Klíčová slova anglicky

RNA-POLYMERASE-II; ELONGATION-FACTOR-B; RECRUITS P-TEFB; TRANSCRIPTIONAL ELONGATION; TERMINAL DOMAIN; TARGET GENES; EXPRESSION; ACTIVATION; TAT; DIFFERENTIATION

Štítky

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 7. 2012 05:50, Olga Křížová

Anotace

V originále

The positive transcription elongation factor b (P-TEFb) is essential for the elongation of transcription and cotranscriptional processing by RNA polymerase II. In mammals, it contains predominantly the C-type cyclin cyclin T1 (CycT1) or CycT2 and cyclin-dependent kinase 9 (Cdk9). To determine if these cyclins have redundant functions or affect distinct sets of genes, we genetically inactivated the CycT2 gene (Ccnt2) using the beta-galactosidase-neomycin gene (beta-geo) gene trap technology in the mouse. Visualizing beta-galactosidase during mouse embryogenesis revealed that CycT2 is expressed abundantly during embryogenesis and throughout the organism in the adult. This finding was reflected in the expression of CycT2 in all adult tissues and organs. However, despite numerous matings of heterozygous mice, we observed no CycT2(-/-) embryos, pups, or adult mice. This early lethality could have resulted from decreased expression of critical genes, which were revealed by short interfering RNAs against CycT2 in embryonic stem cells. Thus, CycT1 and CycT2 are not redundant, and these different P-TEFb complexes regulate subsets of distinct genes that are important for embryonic development.

Citovat

KOHOUTEK, Jiří, Qintong LI, Dalibor BLAŽEK, Zeping LUO, Huimin JIANG a B Matija PETERLIN. Cyclin T2 Is Essential for Mouse Embryogenesis. Molecular and Cellular Biology. Washington, D.C.: ASM, 2009, roč. 29, č. 12, s. 3280-3285. ISSN 0270-7306. Dostupné z: https://dx.doi.org/10.1128/MCB.00172-09.

@article{987838,
   author = {Kohoutek, Jiří and Li, Qintong and Blažek, Dalibor and Luo, Zeping and Jiang, Huimin and Peterlin, B Matija},
   article_location = {Washington, D.C.},
   article_number = {12},
   doi = {http://dx.doi.org/10.1128/MCB.00172-09},
   keywords = {RNA-POLYMERASE-II; ELONGATION-FACTOR-B; RECRUITS P-TEFB; TRANSCRIPTIONAL ELONGATION; TERMINAL DOMAIN; TARGET GENES; EXPRESSION; ACTIVATION; TAT; DIFFERENTIATION},
   language = {eng},
   issn = {0270-7306},
   journal = {Molecular and Cellular Biology},
   title = {Cyclin T2 Is Essential for Mouse Embryogenesis},
   url = {http://mcb.asm.org/content/29/12/3280.full.pdf},
   volume = {29},
   year = {2009}
}

TY  - JOUR
ID  - 987838
AU  - Kohoutek, Jiří - Li, Qintong - Blažek, Dalibor - Luo, Zeping - Jiang, Huimin - Peterlin, B Matija
PY  - 2009
TI  - Cyclin T2 Is Essential for Mouse Embryogenesis
JF  - Molecular and Cellular Biology
VL  - 29
IS  - 12
SP  - 3280-3285
EP  - 3280-3285
PB  - ASM
SN  - 02707306
KW  - RNA-POLYMERASE-II
KW  - ELONGATION-FACTOR-B
KW  - RECRUITS P-TEFB
KW  - TRANSCRIPTIONAL ELONGATION
KW  - TERMINAL DOMAIN
KW  - TARGET GENES
KW  - EXPRESSION
KW  - ACTIVATION
KW  - TAT
KW  - DIFFERENTIATION
UR  - http://mcb.asm.org/content/29/12/3280.full.pdf
N2  - The positive transcription elongation factor b (P-TEFb) is essential for the elongation of transcription and cotranscriptional processing by RNA polymerase II. In mammals, it contains predominantly the C-type cyclin cyclin T1 (CycT1) or CycT2 and cyclin-dependent kinase 9 (Cdk9). To determine if these cyclins have redundant functions or affect distinct sets of genes, we genetically inactivated the CycT2 gene (Ccnt2) using the beta-galactosidase-neomycin gene (beta-geo) gene trap technology in the mouse. Visualizing beta-galactosidase during mouse embryogenesis revealed that CycT2 is expressed abundantly during embryogenesis and throughout the organism in the adult. This finding was reflected in the expression of CycT2 in all adult tissues and organs. However, despite numerous matings of heterozygous mice, we observed no CycT2(-/-) embryos, pups, or adult mice. This early lethality could have resulted from decreased expression of critical genes, which were revealed by short interfering RNAs against CycT2 in embryonic stem cells. Thus, CycT1 and CycT2 are not redundant, and these different P-TEFb complexes regulate subsets of distinct genes that are important for embryonic development.
ER  -

KOHOUTEK, Jiří, Qintong LI, Dalibor BLAŽEK, Zeping LUO, Huimin JIANG a B Matija PETERLIN. Cyclin T2 Is Essential for Mouse Embryogenesis. \textit{Molecular and Cellular Biology}. Washington, D.C.: ASM, 2009, roč.~29, č.~12, s.~3280-3285. ISSN~0270-7306. Dostupné z: https://dx.doi.org/10.1128/MCB.00172-09.

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