In vivo SELEX reveals novel sequence and structural
determinants of Nrd1-Nab3-Sen1 dependent transcription
termination

J 2012

In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination

PORRUA-FUERTE, Odil, Fruzsina HÓBOR, Jocelyne BOULAY, Karel KUBÍČEK, Yves D’AUBENTON-CARAFA et. al.

Základní údaje

Originální název

In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination

Autoři

PORRUA-FUERTE, Odil (724 Španělsko), Fruzsina HÓBOR (348 Maďarsko, domácí), Jocelyne BOULAY (250 Francie), Karel KUBÍČEK (203 Česká republika, domácí), Yves D’AUBENTON-CARAFA (250 Francie), Rajani Kanth GUDIPATI (250 Francie), Richard ŠTEFL (203 Česká republika, domácí) a Domenico LIBRI (250 Francie, garant)

Vydání

EMBO Journal, NEW YORK, NATURE PUBLISHING GROUP, 2012, 0261-4189

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.822

Kód RIV

RIV/00216224:14740/12:00057509

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/emboj.2012.237

UT WoS

000309656600015

Klíčová slova anglicky

cryptic unstable transcripts; transcription termination; Nrd1p-Nab3p-Sen1p complex; hidden transcription; transcriptional insulators

Štítky

ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 4. 2013 08:10, Olga Křížová

Anotace

V originále

The Nrd1-Nab3-Sen1 (NNS) complex pathway is responsible for transcription termination of cryptic unstable transcripts and sn/snoRNAs. The NNS complex recognizes short motifs on the nascent RNA, but the presence of these sequences alone is not sufficient to define a functional terminator. We generated a homogeneous set of several hundreds of artificial, NNS-dependent terminators with an in vivo selection approach. Analysis of these terminators revealed novel and extended sequence determinants for transcription termination and NNS complex binding as well as supermotifs that are critical for termination. Biochemical and structural data revealed that affinity and specificity of RNA recognition by Nab3p relies on induced fit recognition implicating an a-helical extension of the RNA recognition motif. Interestingly, the same motifs can be recognized by the NNS or the mRNA termination complex depending on their position relative to the start of transcription, suggesting that they function as general transcriptional insulators to prevent interference between the non-coding and the coding yeast transcriptomes.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GAP305/10/1490, projekt VaV

Název: Strukturní podstata ukončení transkripce nezávislé na poly(A) signálu

Investor: Grantová agentura ČR, Strukturní podstata ukončení transkripce nezávislé na poly(A) signálu

GBP305/12/G034, projekt VaV

Název: Centrum biologie RNA

Citovat

PORRUA-FUERTE, Odil, Fruzsina HÓBOR, Jocelyne BOULAY, Karel KUBÍČEK, Yves D’AUBENTON-CARAFA, Rajani Kanth GUDIPATI, Richard ŠTEFL a Domenico LIBRI. In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination. EMBO Journal. NEW YORK: NATURE PUBLISHING GROUP, 2012, roč. 31, č. 19, s. 3935-3948. ISSN 0261-4189. Dostupné z: https://dx.doi.org/10.1038/emboj.2012.237.

@article{988334,
   author = {PorruaandFuerte, Odil and Hóbor, Fruzsina and Boulay, Jocelyne and Kubíček, Karel and D’AubentonandCarafa, Yves and Gudipati, Rajani Kanth and Štefl, Richard and Libri, Domenico},
   article_location = {NEW YORK},
   article_number = {19},
   doi = {http://dx.doi.org/10.1038/emboj.2012.237},
   keywords = {cryptic unstable transcripts; transcription termination; Nrd1p-Nab3p-Sen1p complex; hidden transcription; transcriptional insulators},
   language = {eng},
   issn = {0261-4189},
   journal = {EMBO Journal},
   title = {In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination},
   url = {http://www.nature.com/emboj/journal/v31/n19/abs/emboj2012237a.html},
   volume = {31},
   year = {2012}
}

TY  - JOUR
ID  - 988334
AU  - Porrua-Fuerte, Odil - Hóbor, Fruzsina - Boulay, Jocelyne - Kubíček, Karel - D’Aubenton-Carafa, Yves - Gudipati, Rajani Kanth - Štefl, Richard - Libri, Domenico
PY  - 2012
TI  - In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination
JF  - EMBO Journal
VL  - 31
IS  - 19
SP  - 3935-3948
EP  - 3935-3948
PB  - NATURE PUBLISHING GROUP
SN  - 02614189
KW  - cryptic unstable transcripts
KW  - transcription termination
KW  - Nrd1p-Nab3p-Sen1p complex
KW  - hidden transcription
KW  - transcriptional insulators
UR  - http://www.nature.com/emboj/journal/v31/n19/abs/emboj2012237a.html
L2  - http://www.nature.com/emboj/journal/v31/n19/abs/emboj2012237a.html
N2  - The Nrd1-Nab3-Sen1 (NNS) complex pathway is responsible for transcription termination of cryptic unstable transcripts and sn/snoRNAs. The NNS complex recognizes short motifs on the nascent RNA, but the presence of these sequences alone is not sufficient to define a functional terminator. We generated a homogeneous set of several hundreds of artificial, NNS-dependent terminators with an in vivo selection approach. Analysis of these terminators revealed novel and extended sequence determinants for transcription termination and NNS complex binding as well as supermotifs that are critical for termination. Biochemical and structural data revealed that affinity and specificity of RNA recognition by Nab3p relies on induced fit recognition implicating an a-helical extension of the RNA recognition motif. Interestingly, the same motifs can be recognized by the NNS or the mRNA termination complex depending on their position relative to the start of transcription, suggesting that they function as general transcriptional insulators to prevent interference between the non-coding and the coding yeast transcriptomes.
ER  -

PORRUA-FUERTE, Odil, Fruzsina HÓBOR, Jocelyne BOULAY, Karel KUBÍČEK, Yves D’AUBENTON-CARAFA, Rajani Kanth GUDIPATI, Richard ŠTEFL a Domenico LIBRI. In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination. \textit{EMBO Journal}. NEW YORK: NATURE PUBLISHING GROUP, 2012, roč.~31, č.~19, s.~3935-3948. ISSN~0261-4189. Dostupné z: https://dx.doi.org/10.1038/emboj.2012.237.

Podrobný výpis o publikaci