Screening a cDNA Library for Protein-Protein Interactions
Directly in Planta

J 2012

Screening a cDNA Library for Protein-Protein Interactions Directly in Planta

LEE, Lan-Ying; Fu-Hui WU; Chen-Tran HSU; Shu-Chen SHEN; Hsuan-Yu YEH et. al.

Základní údaje

Originální název

Screening a cDNA Library for Protein-Protein Interactions Directly in Planta

Autoři

LEE, Lan-Ying; Fu-Hui WU; Chen-Tran HSU; Shu-Chen SHEN; Hsuan-Yu YEH; De-Chih LIAO; Mei-Jane FANG; Nien-Tze LIU; Yu-Chen YEN; Ladislav DOKLÁDAL; Eva SÝKOROVÁ; Stanton B. GELVIN a Choun-Sea LIN

Vydání

Plant Cell, UNITED STATES, AMER SOC PLANT BIOLOGISTS, 2012, 1040-4651

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.251

Kód RIV

RIV/00216224:14740/12:00057108

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1105/tpc.112.097998

UT WoS

000306105400008

Klíčová slova anglicky

BIMOLECULAR FLUORESCENCE COMPLEMENTATION; STRAND TELOMERIC DNA; BINDING-PROTEIN; NUCLEAR IMPORT; IN-PLANTA; T-DNA; VIRD2 PROTEIN; CELLS; TRANSFORMATION; VISUALIZATION

Štítky

ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 3. 2013 06:52, Olga Křížová

Anotace

V originále

Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. T-DNA insertions in the genes represented by some cDNAs revealed five novel Arabidopsis proteins important for Agrobacterium-mediated plant transformation. We also used this cDNA library to confirm VirE2-interacting proteins in orchid (Phalaenopsis amabilis) flowers. Thus, this technology can be applied to several plant species.

Návaznosti

IAA500040801, projekt VaV

Název: Telomery a telomerázy: posun od molekulárně-biologického ke strukturně-biologickému přístupu.

Investor: Akademie věd ČR, Telomery a telomerázy: posun od molekulárně-biologického ke strukturně-biologickému přístupu

Citovat

LEE, Lan-Ying; Fu-Hui WU; Chen-Tran HSU; Shu-Chen SHEN; Hsuan-Yu YEH; De-Chih LIAO; Mei-Jane FANG; Nien-Tze LIU; Yu-Chen YEN; Ladislav DOKLÁDAL; Eva SÝKOROVÁ; Stanton B. GELVIN a Choun-Sea LIN. Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. Plant Cell. UNITED STATES: AMER SOC PLANT BIOLOGISTS, 2012, roč. 24, č. 5, s. 1746-1759. ISSN 1040-4651. Dostupné z: https://doi.org/10.1105/tpc.112.097998.

@article{990393,
   author = {Lee, LanandYing and Wu, FuandHui and Hsu, ChenandTran and Shen, ShuandChen and Yeh, HsuanandYu and Liao, DeandChih and Fang, MeiandJane and Liu, NienandTze and Yen, YuandChen and Dokládal, Ladislav and Sýkorová, Eva and Gelvin, Stanton B. and Lin, ChounandSea},
   article_location = {UNITED STATES},
   article_number = {5},
   doi = {https://doi.org/10.1105/tpc.112.097998},
   keywords = {BIMOLECULAR FLUORESCENCE COMPLEMENTATION; STRAND TELOMERIC DNA; BINDING-PROTEIN; NUCLEAR IMPORT; IN-PLANTA; T-DNA; VIRD2 PROTEIN; CELLS; TRANSFORMATION; VISUALIZATION},
   language = {eng},
   issn = {1040-4651},
   journal = {Plant Cell},
   title = {Screening a cDNA Library for Protein-Protein Interactions Directly in Planta},
   url = {http://www.plantcell.org/content/24/5/1746.abstract},
   volume = {24},
   year = {2012}
}

TY  - JOUR
ID  - 990393
AU  - Lee, Lan-Ying - Wu, Fu-Hui - Hsu, Chen-Tran - Shen, Shu-Chen - Yeh, Hsuan-Yu - Liao, De-Chih - Fang, Mei-Jane - Liu, Nien-Tze - Yen, Yu-Chen - Dokládal, Ladislav - Sýkorová, Eva - Gelvin, Stanton B. - Lin, Choun-Sea
PY  - 2012
TI  - Screening a cDNA Library for Protein-Protein Interactions Directly in Planta
JF  - Plant Cell
VL  - 24
IS  - 5
SP  - 1746-1759
EP  - 1746-1759
PB  - AMER SOC PLANT BIOLOGISTS
SN  - 10404651
KW  - BIMOLECULAR FLUORESCENCE COMPLEMENTATION
KW  - STRAND TELOMERIC DNA
KW  - BINDING-PROTEIN
KW  - NUCLEAR IMPORT
KW  - IN-PLANTA
KW  - T-DNA
KW  - VIRD2 PROTEIN
KW  - CELLS
KW  - TRANSFORMATION
KW  - VISUALIZATION
UR  - http://www.plantcell.org/content/24/5/1746.abstract
L2  - http://www.plantcell.org/content/24/5/1746.abstract
N2  - Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. T-DNA insertions in the genes represented by some cDNAs revealed five novel Arabidopsis proteins important for Agrobacterium-mediated plant transformation. We also used this cDNA library to confirm VirE2-interacting proteins in orchid (Phalaenopsis amabilis) flowers. Thus, this technology can be applied to several plant species.
ER  -

LEE, Lan-Ying; Fu-Hui WU; Chen-Tran HSU; Shu-Chen SHEN; Hsuan-Yu YEH; De-Chih LIAO; Mei-Jane FANG; Nien-Tze LIU; Yu-Chen YEN; Ladislav DOKLÁDAL; Eva SÝKOROVÁ; Stanton B. GELVIN a Choun-Sea LIN. Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. \textit{Plant Cell}. UNITED STATES: AMER SOC PLANT BIOLOGISTS, 2012, roč.~24, č.~5, s.~1746-1759. ISSN~1040-4651. Dostupné z: https://doi.org/10.1105/tpc.112.097998.

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