| MIKULIKOVA, Ivana, Helena MODRÁ, Jana BLAHOVA, Petr MARSALEK, Ladislav GROCH, Zuzana SIROKA, Kamila KRUŽÍKOVÁ, Jiří JARKOVSKÝ, Simona LITTNEROVÁ a Zdenka SVOBODOVA. The effects of Click 500 SC (terbuthylazine) on common carp Cyprinus carpio under (sub)chronic conditions. Neuroendocrinology Letters. Sweden, Stockholm: Maghira and Maas Publications, 2011, roč. 32, Suppl.1, s. 15-24. ISSN 0172-780X. |
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@article{992771,
author = {Mikulikova, Ivana and Modrá, Helena and Blahova, Jana and Marsalek, Petr and Groch, Ladislav and Siroka, Zuzana and Kružíková, Kamila and Jarkovský, Jiří and Littnerová, Simona and Svobodova, Zdenka},
article_location = {Sweden, Stockholm},
article_number = {Suppl.1},
keywords = {herbicide; fish; biometric indices; biochemical indices; xenobiotic metabolizing enzymes; haematological indices; antioxidant indices},
language = {eng},
issn = {0172-780X},
journal = {Neuroendocrinology Letters},
title = {The effects of Click 500 SC (terbuthylazine) on common carp Cyprinus carpio under (sub)chronic conditions},
volume = {32},
year = {2011}
}
TY - JOUR
ID - 992771
AU - Mikulikova, Ivana - Modrá, Helena - Blahova, Jana - Marsalek, Petr - Groch, Ladislav - Siroka, Zuzana - Kružíková, Kamila - Jarkovský, Jiří - Littnerová, Simona - Svobodova, Zdenka
PY - 2011
TI - The effects of Click 500 SC (terbuthylazine) on common carp Cyprinus carpio under (sub)chronic conditions
JF - Neuroendocrinology Letters
VL - 32
IS - Suppl.1
SP - 15-24
EP - 15-24
PB - Maghira and Maas Publications
SN - 0172780X
KW - herbicide
KW - fish
KW - biometric indices
KW - biochemical indices
KW - xenobiotic metabolizing enzymes
KW - haematological indices
KW - antioxidant indices
N2 - OBJECTIVES: Effects of the herbicide formulation Click 500 SC (terbuthylazine 500 g/l) on common carp Cyprinus carpio were assessed through biometric, biochemical, haematological and antioxidant indices, induction of xenobiotic metabolizing enzymes and histological examination of selected tissues. DESIGN: The fish were exposed to the formulation with terbuthylazine concentrations of 380 ng/l (environmental concentration); 60 mu g/l and 550 mu g/l for up to 91 days. Haematological indices were assessed using unified methods of haematological examination in fish. Biochemical indices in plasma were measured by biochemical analyzer, ferric reducing ability of plasma (FRAP) and ceruloplasmin activity were determined spectrophotometrically. Concentration of total cytochrome P450, glutathione-S-transferase activity and glutathione content were assessed spectrophotometrically in liver. Activity of liver ethoxyresorufin-O-deethylase (EROD) activity was measured spectrofluorimetrically. Histopathological examination of liver, skin, gills, spleen, cranial and caudal kidney was performed by light microscopy. RESULTS: An increase (p<0.05) was observed in hepatosomatic index and condition factor in fish from the environmental concentration. A decrease (p<0.05) in haemoglobin and mean corpuscular haemoglobin concentration (MCHC) was found in fish treated with terbuthylazine of 550 mu g/l. There was a decline in mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) (p<0.05) in terbuthylazine of 60 mu g/l and 550 mu g/l. Triglycerides (TAG) (p<0.01) were elevated in all pesticide-treated groups. Alanine aminotransferase (ALT) (p<0.01) and phosphorus (p<0.05) decreased in fish exposed to terbuthylazine of 60 mu g/l and 550 mu g/l, while albumin (p<0.01) rised in the same groups. An elevation in natrium (p<0.05) in terbuthylazine of 550 mu g/l and a rise in protein (p<0.01) in the concentrations of 380 ng/1 and 550 mu g/l were observed. Correlations between several indices were significant. Ceruloplasmin activity and FRAP were augmented (p<0.01) in the highest concentration tested. Examined xenobiotic detoxification systems were not significantly affected by the exposure. Non-specific histopathological changes were found in the gills and skin of the test fish. CONCLUSION: The fish treated with terbuthylazine developed a disorder in several haematological and plasma biochemical indices. The levels of markers of oxidative stress increased in response to the exposure. Examined systems involved in detoxification of xenobiotics did not reflect long-term contact with the herbicide. Detected histological lesions were non-specific. The environmental concentration of terbuthylazin affected biometric indices of the test fish.
ER -
MIKULIKOVA, Ivana, Helena MODRÁ, Jana BLAHOVA, Petr MARSALEK, Ladislav GROCH, Zuzana SIROKA, Kamila KRUŽÍKOVÁ, Jiří JARKOVSKÝ, Simona LITTNEROVÁ a Zdenka SVOBODOVA. The effects of Click 500 SC (terbuthylazine) on common carp Cyprinus carpio under (sub)chronic conditions. \textit{Neuroendocrinology Letters}. Sweden, Stockholm: Maghira and Maas Publications, 2011, roč.~32, Suppl.1, s.~15-24. ISSN~0172-780X.
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