The introduction of probiotics in the prevention or adjunct therapy of oral diseases represents a current trend in oral medicine. Multiplex qPCR appears to be a suitable method for detecting specific bacterial strains introduced through the use of, for example, oral hygiene products.

The Bachelor's thesis aims to conduct a literature search focusing on oral probiotics, design primers, and test reaction conditions for the analysis of several selected oral bacteria. In the practical part of the work, whole genome sequencing of selected bacterial cultures will be performed and multiplex qPCR will be introduced. In the pilot study, DNA will be isolated from participants and the oral microbiome analyzed using an established method.