Abstract

This master thesis is focused on RNA Interference (RNAi) effect and its usage in gene silencing. RNAi uses a special very short subsequence of silenced mRNA sequence. It is possible to find usable subsequence on its pursuant biochemical properties and structural characteristics. Specifically, this work studies relationship between mentioned theoretically calculated property of subsequences and their efficiency for RNAi for any input set of experimental data. Furthermore, in this work software RNA Interference Analyzer is designed, implemented, tested and used. The Analyzer computes biochemical or structural property for input sets of experimental data (mRNA sequences, their subsequences and efficiency of subsequences). According to this property the Analyzer makes statistical analyses which are displayed and offers the ability to download results for next usage. By this software, a set of analyses for data from CGB and Sonnhammer databases was realized. In these analyses, factual dependence of property value on subsequence RNAi efficiency was verified. These results fit outcomes presented in literature which validate usability of the Analyzer. …víceméně