Thesis/Dissertation: Jana Řezáčová: Stereospecificity of enzymatic oxidation of NADH
Bachelor's thesis
Stereospecificity of enzymatic oxidation of NADH
Stereospecifita enzymové oxidace NADH
Abstract
Oxidoreduktázy spojené s pyridinovými nukleotidy jsou široká třída enzymů, které zpravidla stereospecificky přenášejí hydrid ze substrátu na pozici C4 pyridinu NAD+ nebo NADP+ a naopak. U velkého množství enzymů již byla zjištěna jejich stereospecifita a tyto výsledky můžeme nalézt v databázích. V dnešní době se pro účel určení stereospecifity nejvíce využívá metoda 1H-NMR. Cílem této práce je určit stereospecifitu enzymu FerB izolovaného z bakterie Paracoccus denitrificans.
Abstract
The pyridine nucleotide linked oxidoreductases are an extensive class of enzymes which are capable of the ordinarily stereospecifically transfer of hydride from a substrate to the pyridine 4 position of NAD+ or NADP+ and vice versa. Stereospecifity has been already found at lagre numbers of enzymes and we can find results in databases. Nowadays 1H-NMR technique is most fequetnly used technique for …more
11/10/2008 13:02, (IS automatically)
- Entered/Edited 30/6/2008 15:33, Jindřiška Chlebečková
- Record made 27/3/2008 13:24, Jindřiška Chlebečková
- Accessible from: 29/5/2008 11:19, Jindřiška Chlebečková
- Thesis/dissertation received 29/5/2008 11:19, Jindřiška Chlebečková
Supervisor
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