Centrifugal partition chromatography embodies hydrostatic countercurrent chromatography exploiting the principle of partitioning the compounds between two immiscible liquid phases. One of the phases, used as the stationary phase, is immobilized in a rotating column by centrifugal forces, while the other is pumped through the column serving as the mobile phase. CPC constitutes a very versatile method because a wide spectrum of solvents and various operating conditions can be used, allowing the user to separate from the level of proteins up to small organic molecules. In this Bachelor’s project, the impact of different operating conditions, such as temperature, rotation speed, and flow-rate, on separation will be examined. The aim of the project is to analyze the dependency of the quality of separation on diverse operating conditions, with possible application of statistical tools and computer modeling.