EMAR0832c Methods of Assisted Reproduction II - practice

Faculty of Medicine
spring 2025
Extent and Intensity
0/4/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. MVDr. Aleš Hampl, CSc. (seminar tutor)
PharmDr. Zuzana Holubcová, Ph.D. (seminar tutor)
MUDr. Eva Mecová (seminar tutor)
MUDr. Volodymyr Porokh (seminar tutor)
RNDr. Gabriela Tauwinklová (seminar tutor)
Drahomíra Kyjovská (assistant)
Guaranteed by
doc. MVDr. Aleš Hampl, CSc.
Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Gabriela Tauwinklová
Supplier department: Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main goal of the course is to familiarize students with the practical aspects of basic and special methods of assisted reproduction. During the exercises, students will learn micromanipulation with gametes and embryos, laboratory methods performed during the acquisition of gametes, their evaluation, and preparation for fertilization. Great attention will be paid to the practical training of fertilization methods, evaluation of embryo quality, cryopreservation of gametes and embryos, and sample collection for genetic testing. A key element of the course will be the practical training of skills.
Learning outcomes
After completing the course, the student will be able to: Master the procedures for obtaining gametes and preparing them for fertilization. Gain experience in performing micromanipulation techniques, including methods of oocyte fertilization using intracytoplasmic sperm injection (ICSI) and sample collection for genetic testing. Independently assess the quality of gametes and understand the methods for evaluating embryo development. Know how to prepare an embryo for transfer and understand the basic procedures for cryopreservation of gametes and embryos. Adjust a microscope and micromanipulator.
Syllabus
  • Basic and special methods of assisted reproduction (AR) Processing (ejaculate/testicular biopsy), preparation of sperm for in vitro fertilization, determination and evaluation of spermiogram Searching for and evaluating oocyte-cumulus complexes, denudation of oocytes, assessment of oocyte maturity and quality Conventional in vitro fertilization Fertilization using intracytoplasmic sperm injection (ICSI) and its modifications (PICSI, IMSI) Classical and extended embryo culture, individual and group culture, assisted hatching Evaluation of embryo development – basic morphological criteria, assessment of morphokinetic parameters (Time-lapse systems) Embryo transfer – transfer techniques, media, transfer catheters, proper passage during transfer Cryopreservation methods (slow freezing, vitrification; open and closed freezing systems), practical training in freezing and thawing sperm and embryos; safety working with liquid nitrogen Laboratory documentation management
Literature
    recommended literature
  • WHO laboratory manual for the examination and processing of human semen, 5th ed., 2010.
  • Coward K., Wells D.: Textbook of Clinical Embryology, 1st ed., Cambridge 2013.
  • Elder K.: In Vitro Fertilization. 3rd ed. Cambridge 2011.
  • Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology:
  • Magli, C, Jones,G.M., Lundin,K., Van den Abbeel E. and The Special Interest Group on Embryology: Atlas 27, Suppl. 1.
  • http://atlas.eshre.eu/
  • The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. In Hum Reprod. 2011 Jun;26(6):1270-83.
  • In vitro fertilization. Edited by Kay Elder - Brian Dale. 2nd ed. New York: Cambridge University Press, 2000, xii, 310 p. ISBN 0521778638. info
Teaching methods
The main element of the course will be practical training in the teaching laboratory, supplemented by an excursion to a clinical embryology laboratory. During the practical training, mouse oocytes, embryos, and reproductive organs (or biological material from other animal species) will be used.
Assessment methods
Attendance at the practice, credit + practical exam
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on the extent and intensity of the course: 60.
The course is also listed under the following terms spring 2024.
  • Enrolment Statistics (spring 2025, recent)
  • Permalink: https://is.muni.cz/course/med/spring2025/EMAR0832c