aVLHE0221c Histology and Embryology I - practice

Faculty of Medicine
spring 2020
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
MVDr. Martin Anger, CSc. (seminar tutor)
MUDr. Ivana Baltasová (seminar tutor)
Mgr. Michaela Capandová (seminar tutor)
prof. MUDr. RNDr. Svatopluk Čech, DrSc. (seminar tutor)
MUDr. Jana Dumková, Ph.D. (seminar tutor)
doc. MVDr. Aleš Hampl, CSc. (seminar tutor)
Mgr. Hana Kotasová, Ph.D. (seminar tutor)
doc. RNDr. Irena Koutná, Ph.D. (seminar tutor)
MUDr. Irena Lauschová, Ph.D. (seminar tutor)
MVDr. Anna Mac Gillavry Danylevska, Ph.D. (seminar tutor)
MUDr. Eva Mecová (seminar tutor)
doc. MUDr. Miroslava Sedláčková, CSc. (seminar tutor)
MUDr. Veronika Sedláková, Ph.D. (seminar tutor)
RNDr. Petr Vaňhara, Ph.D. (seminar tutor)
Guaranteed by
doc. MVDr. Aleš Hampl, CSc.
Department of Histology and Embryology - Theoretical Departments - Faculty of Medicine
Contact Person: doc. MUDr. Miroslava Sedláčková, CSc.
Supplier department: Department of Histology and Embryology - Theoretical Departments - Faculty of Medicine
Timetable of Seminar Groups
aVLHE0221c/30: Mon 17. 2. to Fri 15. 5. Fri 7:30–10:00 N01022/22
aVLHE0221c/31: Mon 17. 2. to Fri 15. 5. Fri 7:30–10:00 N01022/22
aVLHE0221c/32: Mon 17. 2. to Wed 13. 5. Wed 7:30–10:00 N01022/22
aVLHE0221c/33: Mon 17. 2. to Wed 13. 5. Wed 7:30–10:00 N01022/22
aVLHE0221c/34: Mon 17. 2. to Wed 13. 5. Wed 7:30–10:00 N01022/22
aVLHE0221c/35: Mon 17. 2. to Mon 11. 5. Mon 7:30–10:00 N01022/22
aVLHE0221c/36: Mon 17. 2. to Mon 11. 5. Mon 7:30–10:00 N01022/22
aVLHE0221c/37: Mon 17. 2. to Mon 11. 5. Mon 7:30–10:00 N01022/22
aVLHE0221c/38: Mon 17. 2. to Thu 14. 5. Thu 14:00–16:30 N01022/22
aVLHE0221c/39: Mon 17. 2. to Thu 14. 5. Thu 14:00–16:30 N01022/22
Prerequisites (in Czech)
aVLAN0121s Anatomy I -sem. && aVLBI0121c Biology I - pract.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course objectives
At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy.
2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue.
3. Identify tissue types in histologic slides.
4. Characterize microscopic structure of some organs of human body and explain correlations of tissues within organs.
5. Describe gamete development, explain principle of meiosis and regulation of gametogenesis.
6. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord.
7. Characterize stages of embryonic development.
Learning outcomes
At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy.
2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue.
3. Identify tissue types in histologic slides.
4. Characterize microscopic structure of some organs of human body and explain correlations of tissues within organs.
5. Describe gamete development, explain principle of meiosis and regulation of gametogenesis.
6. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord.
7. Characterize stages of embryonic development.
Syllabus
  • 1. Introduction, organization of practicals, teaching aids. Overview of histological methods. Tissue processing for light and electron microscopy - explanation and film. Basic histochemistry and immunocytochemistry.
  • 2. Cytology: Ultrastructure of the cell nucleus during interphase. Ultrastructure of cell organelles I (mitochondria, the Golgi apparatus, the endoplasmic reticulum, ribosomes, lysosomes).
  • 3. Ultrastructure of cell organelles II (peroxisomes, and the centriol). Cell inclusions. The arrangement of cell surfaces: apical, lateral, and basal ones. Intercellular junctions: adhering, occluding, and communicating ones.
  • 4. General embryology I. Aids: Set of embryological schemes and pictures (I).
  • 5. General embryology II. Aids: Set of embryological schemes and pictures (II).
  • 6. General histology: Use of the light microscope, common faults in microscopy. Results of basic staining methods in histology: Hematoxylin-eosine /HE/ (hepar), hematoxylin–eosine-saffron /HES/ (esophagus), AZAN (prostate), impregnation (cerebellum). Connective tissue proper - classification and examples. Slides: Funiculus umbilicalis, Esophagus, Posterior segment of the eye, Lien, Aorta.
  • 7. Supporting tissues: cartilage and bone. Histogenesis of the bone tissue (ossification). Slides: Trachea, Auricula, Elastic cartilage, Lamellar bone, Chondrogenic ossification.
  • 8. Epithelial tissue: epithelial membranes (covering epithelia) and glandular epithelium. Slides: Ren, Vesica fellea, Trachea, Esophagus, Ureter, Palpebra, Skin from the finger tip.
  • 9. Glandular epithelium. Skides: Intestinum tenue, Glandula parotis, Glandula submandibularis.
  • 10. Nerve tissue. Slides: Cortex cerebri, Cerebellum, Medulla spinalis, Ganglion spinale, Peripheral nerve, Motor end plate.
  • 11. Muscle tissue. Slides: Apex linguae, Cardiac muscle tissue Intestinum tenue.
  • 12. Blood cells: Preparation of blood smears and their staining. Cytology of formed elements of blood. Differential white cell count (dWCC) - average values, abnormities and terminology of dWCC. Slide: A smear of peripheral blood stained by Pappenheim's method. Credits.
Literature
    not specified
  • EROSCHENKO, Victor P. Di Fiore's atlas of histology with functional correlations. 9th ed. Philadelphia: Lippincott Williams-Wilkins, 2000. xv, 363. ISBN 0683307495. info
Teaching methods
Content of this course includes study of slides and electron micrographs in microscopic hall as well as class discussion on the base of materials in the text book. Electronic textbooks are presented also in: http://www.med.muni.cz/histol/atlas.htm
Assessment methods
Testing of knowledge: Student must prove sufficient level of knowledge by written test examination. Each student completes 4 partial tests during semester. Tests are evaluated by point for correct answer. More than half number of correct answers (points) is evaluated as "Passed". All of these tests must be successful. In case of failure, only 1 resit is possible. There is condition 4 from 4 (ie. 4 P / 4 regular tests) or 4 from 5 (ie. 3 P / 4 regular tests + 1 P / 1 resit). If student does not fulfill this condition, credit test follows in the relevant exam period. This test covers all topics studied during semester. In case of failure in credit test, credit will not be given and student must enroll the course again.
Conditions for obtaining credit: 1. Attendance at all practical exercises (100% participation, all absences must be regularly excused (in IS) and substituted).
2. Successful completion of all tests.
3. Submission of all protocols (correctly completed forms of protocols signed by teacher).
Language of instruction
English
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2016, Spring 2017, Spring 2018, spring 2019.
  • Enrolment Statistics (recent)
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