LF:BLLK051c Liquorology - practice - Course Information

BLLK051c Liquorology - practice

Extent and Intensity

0/1/0. 1 credit(s). Type of Completion: z (credit).

Teacher(s)

MUDr. Zdeňka Čermáková, Ph.D. (seminar tutor)
Michaela Gregorovičová (assistant)

Guaranteed by

doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine

Timetable of Seminar Groups

BLLK051c/01: Mon 15:20–16:10 342
BLLK051c/02: Thu 15:00–15:50 342

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Laboratory Assistant (programme LF, B-SZ)

Course objectives

The goal of theoretical part considering the subject of laboratory diagnosis of neurological diseases is to become familier with the modern conception of cerebrospinal fluid analysis. Sensitive immunochemical methods improve to make diagnosis of inflammatory and degenerative damages of nervous system. It is neccesssary to provide information related to indications and above all interpretations of results of the particular methods considering clinical units. In the practical part students gain experience with executing particular methods in cerebrospinal fluid analysis at least within the range that the graduate is able to join the working team of laboratory dealing with diagnosis of neurological diseases.; A student will be able to do the following upon completing the course: perform cytological tests of cerebrospinal fluid – quantitative cytology in a FR chamber; prepare cytospin dissections – qualitative cytology; perform and evaluate biochemical tests of cerebrospinal fluid including spectrophotometry; apply immunochemical methods of testing cerebrospinal fluid including isoelectric focusing of CSF and serum;

Syllabus

• Reception and identification of biological material ; Laboratory information systém; CSF cells count in Fuchs-Rosenthall cell ; Cellspin – cytocentrifugation- cell differential; Biochemical analysis of CSF ; Immunochemical analysis of CSF ; Spectrophotometry of CSF ; Isoelectric focusation of CSF and blood ; Demonstration of liquorea ;

Literature

• DASTYCH, Milan, Zdeňka ČERMÁKOVÁ, Pavel ŠTOURAČ, Alena ŠEVČÍKOVÁ, Iva KOCMANOVÁ and Svatava SNOPKOVÁ. Mikroskopický nález kryptokoka v mozkomíšním moku jako první příznak rozvinutého stadia AIDS: kazuistika. (Microskopis detection of Cryptococcus in the cerebrospinal fluid as the first symptom of the advanced stage of AIDS - a case study.). Česká a slovenská neurologie a neurochirurgie. Brno: Medica Healthworld, 2008, 71/104, No 1, p. 93-96. ISSN 1802-4041. info

Teaching methods

Continuous oversight of the assigned tasks, possible corrections and evaluation are performed by the lecturer;

Assessment methods

credit based on active attendance at practical laboratory work, written protocols and verification of attained knowledge and practical skills;

Language of instruction

Czech

Further Comments

Study Materials

• Enrolment Statistics (Autumn 2016, recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2016/BLLK051c