BLLC0111c Medical Chemistry - practice

Faculty of Medicine
autumn 2025
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. David Galuška (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Iva Audy (assistant)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
BLLC0111c/Sk_1: Tue 16. 9. 8:00–10:30 A16/211, Tue 23. 9. 8:00–10:30 A16/211, Tue 30. 9. 8:00–10:30 A16/211, Tue 7. 10. 8:00–10:30 A16/211, Tue 14. 10. 8:00–10:30 A16/211, Tue 21. 10. 8:00–10:30 A16/211, Tue 4. 11. 8:00–10:30 A16/211, Tue 11. 11. 8:00–10:30 A16/211, Tue 18. 11. 8:00–10:30 A16/211, Tue 25. 11. 8:00–10:30 A16/211, Tue 2. 12. 8:00–10:30 A16/211, Tue 9. 12. 8:00–10:30 A16/211, Tue 16. 12. 8:00–10:30 A16/211
BLLC0111c/Sk_2: Tue 16. 9. 8:00–10:30 A16/216, Tue 23. 9. 8:00–10:30 A16/216, Tue 30. 9. 8:00–10:30 A16/216, Tue 7. 10. 8:00–10:30 A16/216, Tue 14. 10. 8:00–10:30 A16/216, Tue 21. 10. 8:00–10:30 A16/216, Tue 4. 11. 8:00–10:30 A16/216, Tue 11. 11. 8:00–10:30 A16/216, Tue 18. 11. 8:00–10:30 A16/216, Tue 25. 11. 8:00–10:30 A16/216, Tue 2. 12. 8:00–10:30 A16/216, Tue 9. 12. 8:00–10:30 A16/216, Tue 16. 12. 8:00–10:30 A16/216
Prerequisites
Participation in the course is conditional on the successful completion of the course ZC011.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objectives of the course are to become oriented in the environment of the chemical laboratory and its equipment, to acquire simple laboratory procedures and techniques, to practice common separation procedures and become familiar with handling standard laboratory machines, to teach students how to process measurement results into protocols, evaluate them and draw conclusions.
Learning outcomes
Upon completion of the course, the student will be able to
- understand the most important bioanalytical principles
- manipulate basic laboratory tools and equipment
- pipetting and weighing according to the principles of good laboratory practice
- prepare a solution at a given concentration
- measure the pH of solutions, titrate
- prepare a suitable buffer and determine its buffering capacity
- master basic separation techniques - filtration, dialysis, centrifugation, extraction, chromatography
- perform basic qualitative analysis - precipitation, colour reactions
- apply spectrophotometry for bioanalytical purposes
Syllabus
  • Safety and health protection when working in the laboratory, first aid principles.
  • Measuring containers/tools. Heating, cooling, weighing. Pressure bottles.
  • Measuring small volumes of liquids, pipettor calibration.
  • Preparation of solutions of a given concentration.
  • Filtration, dialysis, ultrafiltration, centrifugation. Concentration of samples.
  • Measuring pH of solutions, calibration of pH meters.
  • Neutralization volumetric analysis. Titration neutralization curves.
  • Preparation of buffers, determination of buffer capacity.
  • Ionic reactions, ion-selective electrodes, precipitation reactions.
  • Chromatography.
  • Spectrophotometry.
  • Oxidation of alcohols, evidence of aldehydes/ketones, color reactions of amino acids.
  • Protein solubility, denaturation, methods of protein quantification.
Literature
    required literature
  • Interaktivní osnova v IS MU.
Teaching methods
Practical exercises.
Assessment methods
Course-unit credit. Conditions for giving course-unit credit:
1) Completion of all exercises, incl. submission of all lab reports and 2) Completion of progress tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
A white coat, slippers or sturdy shoes with overshoes, gloves, and a calculator are required for the practical class.

Detailed information on the teaching and completion of the course is given in the Study Materials in IS MU.

The course is also listed under the following terms Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/med/autumn2025/BLLC0111c