EMBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2026
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. David Galuška (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Iva Audy (assistant)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Prerequisites (in Czech)
EMBF0211c Biophysics - practice && EMBC0321s Biochemistry I - seminar && EMFY0321c Physiology I - practicals && EMFY0321s Physiology I - practicals
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is complementary to the course EMBC0422p. The aim of the course is to acquaint students with the basic methodological principles and procedures used in determining the concentration of analytes and enzyme activity in body fluids and methods of interpretation of laboratory results.
Learning outcomes
After completing the course, the student will be able to:
- follow the principles of working with biological material
- be familiar with basic laboratory tasks
- explain methods of obtaining biological material for biochemical analyzes
- describe the basic methodological principles and procedures used in determining the concentration of analytes and enzyme activity in body fluids
- interpret the results of biochemical analyzes, discuss the significance of the findings
Syllabus
  • Laboratory examination. Blood, plasma, serum - factors influencing the measurement result, interpretation
  • Blood, plasma, serum - blood collection and processing
  • Diagnostic accuracy of laboratory testing - sensitivity, specificity, predictive values, prevalence.
  • Laboratory diagnosis of myocardial infarction - B-cTnT
  • Selected examinations of glucose metabolism - P/U-glucose, OGTT, U-ketone bodies, S-HbA1
  • Selected examinations of lipid and cholesterol metabolism - S/B-cholesterol, S-TG, S-LDL/HDL/non-HDL-cholesterol.
  • Selected liver examinations - S-ALT/AST, S/U-bilirubin, U-UBG, S-albumin
  • Selected examinations of proteins in blood serum - S-total protein, S-albumin, electrophoresis, S-CRP, ELISA
  • Selected kidney examinations - S/U-creatinine, creatinine clearance, eGFR, FR and FE water, U-proteins, U-albumin, U-blood, S/U-urea, nitrogen balance
  • Chemical qualitative analysis of urine - diagnostic strips
  • Selected examinations of urolithiasis - S/U-uric acid, chemical analysis of stones
Literature
    required literature
  • Interaktivní osnova v IS MU.
Teaching methods
Lab exercises
Assessment methods
Course-unit credit. Conditions for giving the course-unit credit:
1) completion of all classes, 2) elaboration and approval of all protocols, 3) completion of the final test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught every week.
General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu EMBC0422p.
Listed among pre-requisites of other courses
Teacher's information
Detailed information on the teaching and completion of the course is given in the Study Materials in IS MU.

White coat, slippers or sturdy shoes with covers, gloves, calculator are required for the exercise.

Consultation is possible by personal arrangement with the instructor.

The course is also listed under the following terms spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (spring 2026, recent)
  • Permalink: https://is.muni.cz/course/med/spring2026/EMBC0422c