Bi2MP_ZMIC Basic of microbiology

Faculty of Education
Autumn 2011
Extent and Intensity
0/0/2. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Marek David, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Marek David, Ph.D.
Department of Biology – Faculty of Education
Contact Person: Mgr. Marek David, Ph.D.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to: - explain basic terms used in microbiology, clarify main differences among individual groups of microorganisms, mention their basic taxonomic classification and enumerate their typical species;
- describe individual work instruments used in microbiological laboratory and interpret their characteristic functions;
- prepare culture media for isolation, growth and storage of microorganisms;
- apply basic microbiological methods (isolation, cultivation and identification of microorganisms, inoculation, observation of microorganisms by microscope etc.);
- understand an importance of sterile work in microbiological laboratory;
- characterize generally an occurrence of microorganisms in environment (soil, water), their contribution to nutrients flow in environment and their significance for man (etiological agens of infectious diseases, commensals, using of microorganisms in industry and in research, bioremediation)
Syllabus
  • 1. Introduction: Labour safety. Microbiology as a branch of science, historical milestones of microbiology and its significance today. Basic classification of microorganisms. Fundamental principles of work with microorganisms, characterization of working tools and instrumentation. Culture mediums preparation. Sterilization.
  • 2. Characterization of prokaryotic cell. ABC of bacterial taxonomy. Inoculation and cultivation of microorganisms.
  • 3. Isolation (streaking) and identification of microorganisms. Microscopy and microorganisms I. - kinds of microscopes, microscopic techniques, principles of work with microscopes. Observation of microorganisms.
  • 4. Assessment of microorganisms’ colonies morphology. Microscopy and microorganisms II. - native, fixed and permanent slides, microorganisms staining (Gram staining, etc.).
  • 5. Effect of environment on microorganisms. Cell cycle of prokaryotic organisms. Methods of cell amount assessment in microbial culture.
  • 6. Importance of bacteria for man I. - pathogenic and non-pathogenic bacteria, using of microorganisms in research. Microorganisms ecology I. - microbiological assessment of water pollution.
  • 7. Importance of bacteria for man II. - using of bacteria in industry, bioremediation. Microorganisms ecology II. - bacteria isolation from soil, microbiological analysis of soil and food.
  • 8. Yeasts: their importance for man (fermentative processes, model organisms, pathogens). Cultivation, identification and microscopic observation of yeasts.
  • 9. Practical microbiology - excursion of chosen microbiological workplace (will be specified during semester). Introduction to veterinary microbiology. Other microscopic organisms – viruses, fungi, parasites.
  • 10. Internet sources, collections of microorganisms, protocols control.
Literature
    recommended literature
  • JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info
  • VESELÁ, Mária and Milan DRDÁK. Praktikum z obecné mikrobiologie. Vyd. 2. přeprac. Brno: Vysoké učení technické v Brně, 1999, 87 s. ISBN 80-214-1305-0. info
    not specified
  • Vybrané metody v mikrobiologii. Edited by Josef Cudlín. Vyd. 1. Praha: Academia, 1981, 488 s. URL info
  • KOCKOVÁ-KRATOCHVÍLOVÁ, A, G BASAŘOVÁ, Olga BENDOVÁ, Karel BERAN, V BETINA, M BETINOVÁ, P BIELY, V FARKAŠ, Marie HAVELKOVÁ, Z HOLAN, M KAHLER, Marie KOPECKÁ, Arnošt KOTYK, V KOVÁČOVÁ, J LIEBLOVÁ, O MARKOVIČ, E MINÁRIK, Oldřich NEČAS, J ŘIČICA, M SOBOTKA, Augustin SVOBODA, Eva STREIBLOVÁ, Josef ŠANDULA, L ŠILHÁNKOVÁ, Jana SUBIKOVÁ, A TOMŠÍKOVÁ and David VRÁNA. Kvasinky ve výzkumu a praxi. 1st ed. Praha: Academia Praha, 1986, 379 pp. Československá akademie věd. URL info
  • NĚMEC, Miroslav and Dana HORÁKOVÁ. Základy mikrobiologie pro učitelské studium. 3. vyd. Brno: Masarykova univerzita v Brně, 1999, 233 s. ISBN 8021020601. info
  • JANDEROVÁ, Blanka and Olga BENDOVÁ. Úvod do biologie kvasinek. 1. vyd. Praha: Karolinum, 1999, 108 s. ISBN 8071849901. info
Teaching methods
theoretical preparation, laboratory course
Assessment methods
Individual laboratory course during whole semester are obligatory for students (accepted is one unexcused absence). Control of filled work protocols by teacher is necessary condition for successful completion of the course. Students acquire credit after oral exam, which has a form of discussion and which include a short practical demonstration.
Language of instruction
Czech
Further Comments
Study Materials
The course is also listed under the following terms Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017.
  • Enrolment Statistics (Autumn 2011, recent)
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