FDFBC_FAF Biochemistry

Faculty of Pharmacy
Spring 2020
Extent and Intensity
1/1/0. 25 credit(s). Type of Completion: zk (examination).
Guaranteed by
Mgr. Marie Brázdová, Ph.D.
Faculty of Pharmacy
Prerequisites
The subject is realized through consultations and self-study, assumes the mastering of the study literature and the understanding of the context.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
Biochemistry is aimed at deepening the knowledge of biochemistry obtained in the undergraduate study. It focuses on detailed information on selected chemical processes in living organisms relevant to the study of DSP Pharmacognosy. Special emphasis is placed on modern information and links between biochemistry and molecular biology.
Syllabus
  • Syllabus Biochemistry
    1) Biochemistry: an introduction
    1.1) Scope of biochemistry: central principles, cellular and chemical foundations (functional groups, reactions), physical foundations
    1.2) Living cells (structure of eukaryotic cell)
    1.3) Water: the medium of life (interactions, pH, pKa, buffers, blood, liver and urine buffering systems, osmosis, dialysis)
    1.4) Energy (free energy, ATP)
    2) Structure and function of proteins
    2.1) Amino acids (structure, pKa, pI, properties, covalent modifications)
    2.2) Peptides (peptide bound, disulfide bridge, glutathione, insulin, antibiotics, neurotransmiters)
    2.3) Protein structure (secondary, tertiary, conformation, structure and function)
    2.4) Structure and function of hemoglobin and myoglobin
    3) Enzymes
    3.1) Properties of Enzymes (cofactors, coenzymes, vitamins)
    3.2) Classification of Enzymes (examples of each class)
    3.3) Catalysis (enzyme reaction, active site, specificity) and Enzyme regulation
    3.4) Kinetics of enzyme reactions
    4) Membrane transport
    4.1) Biomembranes - composition and function
    4.2) Membrane transports
    5) Carbohydrates and metabolism of carbohydrate
    5.1) Monosaccharides, Disaccharides, Oligosaccharides
    5.2) Polysaccharides (structure and function)
    5.3) Metabolism, homeostasis, catabolism, anabolism, nutrient pool, energy metabolism
    5.4) Glycolysis and Glyconeogenesis
    5.5) Glycogen metabolism
    6) Metabolism of Proteins and Amino Acid Nitrogen
    6.1) Overview of metabolism of proteins,
    6.2) Elimination of alfa-amino nitrogen
    6.3) Detoxification of Ammonia
    6.4) Catabolism of Amino Acids (Ala, Arg, Ser, Gly, Thr, Asp, Glu, His, Phe, Tyr, Cys)
    7) Aerobic metabolism
    6.1) Tricarboxy Acid Cycle (Citric Acid Cycle (overview, fuels, mitochondrion, enzymes and coenzymes, 8 steps of CC, regulation of pyruvate dehydrogenase, control of CC, biosynthesis aspects of CC)
    6.2) Respiratory chain (redox potential, sequence of electron carriers)
    6.3) Oxidative phosphorylation (enzymes, prosthetic groups, localization in mitochondria, mechanism ATP synthesis)
    6.4) Oxidative stress (reactive oxygen species, their formation, antioxidant enzymes systems, antioxidant molecules)
    7) Lipids
    7.1) Lipids classes (definition, biological) and metabolism
    7.2) Synthesis and degradation of Fatty acids
    7.3) Lipid synthesis. Peroxidation and Eicosanoids.
    7.4 Biotransformation
    8) Nucleic acids and their metabolism
    8.1) Structure of components, role of nucleotides, biosynthesis (replication, transcription and proteosynthesis
    8.2) Nucleic acids metabolism (biosynthesis and degradation of purine and pyrimidine nucleotides)
    9) Integration of metabolism and cell signaling
Literature
    required literature
  • MURRAY,R.K., GRANNER,D.K.,RODWELL,V.W. Harper´s Illustrated Biochemistry. Appleton & Lange, 2006. ISBN 07-147885-X. info
  • Robert K. Murray, Daryl K. Granner, Peter A. Mayes, Victor W. Ro. Harperova biochemie. ČR, 2002. ISBN 80-7319-013-3. info
  • CHAMPE,P.C.,HARVEY,R.A. Lippincott´s Illustrated Reviews: Biochemistry. Lippincott Wiliams & Wilkins, 1994. ISBN 0-397-51091-8. info
    not specified
  • MURRAY,R.K., GRANNER,D.K.,MAYES,P.A.,RODWELL,V.W. Harperova biochemie (český překlad). Nakladatelství a vydavatelství H & H, 1993. ISBN 80-85787-38-5. info
  • APPS,D.K.,COHEN,B.B.,STEEL,C.M. Biochemistry (A concise text for medical students). Bailliére Tindall, 1992. ISBN 70-20-1444-3. info
Teaching methods (in Czech)
Metody práce s textem (učebnicí, knihou)
Assessment methods (in Czech)
Ústní zkouška
Písemná zkouška
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
Requirements for the student
Successful completion of the subject by written and oral examination. The mark is based mainly on the part of the test. To pass the course, you must pass the test at 60%.
The course is also listed under the following terms Autumn 2020, Spring 2021, Autumn 2021, Spring 2022, Autumn 2022, Spring 2023, Autumn 2023, Spring 2024, Autumn 2024, Spring 2025.
  • Enrolment Statistics (Spring 2020, recent)
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