FDSAa_FAF Separation and Analytical Methods

Faculty of Pharmacy
Spring 2026
Extent and Intensity
2/0/0. 5 credit(s). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
prof. PharmDr. Karel Šmejkal, Ph.D. (lecturer)
Guaranteed by
prof. PharmDr. Karel Šmejkal, Ph.D.
Faculty of Pharmacy
Supplier department: Department of Natural Drugs – Departments – Faculty of Pharmacy
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The Separation Analysis Methods aim to show DSP students modern methods of isolation of natural substances, especially chromatographic techniques. It deepens knowledge of both analytical and preparative separation of secondary metabolites, focusing on specific methods of division and detection. It shows the possibilities of so-called scale-up chromatographic systems, the specifics in the division of different types of substances, and the possibility of verifying the quality of the separation performed.
Learning outcomes
The ability to practically use all the available separation analytical methods, to control their theoretical basics.
Syllabus
Separation analytical methods is a subject showing the modern approaches for the isolation of natural compounds, namely using different chromatographic techniques. It shows possibilities of both analytical and preparative separation, including the scaling up of the methods. It is targeted on specifics of separation of different compounds, chromatographic systems, and also on the control of the quality of the separation process.
1) analytical HPLC
2) semipreparative HPLC
3) analytical TLC
4) preparative TLC
5) column chromatography
6) further separation techniques
Literature
    recommended literature
  • Smejkal, K, Muselik, J, Mokry, P. Laboratory methods of experimental phytochemistry. Brno, 2017. ISBN 978-80-7305-796-1. info
  • Sarker, SD, Latif, Z, Gray, AI. Natural Products Isolation. New jersey, 2006. ISBN 1-59259-955-9. info
  • Walton, NJ, Brown, DE. Chemicals from Plants. London, 1999. ISBN 981-02-2773-6. info
Teaching methods
Discussion, reading, brainstorming, demonstration of methods.
Assessment methods
Oral examination
Language of instruction
English
The course is also listed under the following terms Spring 2025, Autumn 2025.
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