PřF:Bi7001 Cell Architecture - Course Information
Bi7001 Cell Architecture
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
RNDr. Jan Škoda, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Renata Veselská, Ph.D., M.Sc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Renata Veselská, Ph.D., M.Sc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 16:00–17:50 B11/333
- Prerequisites
- Bi1700 Cell Biology && Bi4020 Molecular biology
Knowledge of cell and molecular biology at the bachelor's level. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cell Biology (programme PřF, N-CELLBI)
- Course objectives
- The main objective of this course is to enable students to understand the logic of the 3D-organization of eukaryotic (especially animal) cells and to recognize the mutual spatial relationships among cellular components in the context of their functions.
- Learning outcomes
- After successful completion of this course, the student will be able to: understand the 3D-organization of eukaryotic cells; describe the 3D-structure of cellular components and their spatial relationships; discuss the relationship between the architecture of specific cell types and their functions.
- Syllabus
- 1. THE RULES OF 3D ORGANIZATION INSIDE AND OUTSIDE CELLS: membrane organelles, cytoskeleton, extracellular matrix, CAMs, cell interactions and cell junctions (recap of the basic knowledge).
- 2. CELL NUCLEUS: internal spatial organization of the cell nucleus, nuclear envelope and nuclear lamina, nuclear pores and the communication between cell nucleus and cytoplasm.
- 3. SPATIAL ORGANIZATION OF THE EUKARYOTIC CELL: architecture of cytoplasmic organelles, mutual spatial arrangement of membrane organelles and their functional relationships, vesicular transport.
- 4. BIOGENESIS AND DEGRADATION OF ORGANELLES: overview of the mechanisms and spatial arrangement of organelles biogenesis and degradation, autophagy and its importance for the cell, autophagy, lysosomes, vacuoles.
- 5. MITOCHONDRIA: mitochondrial dynamics, biogenesis, mitochondrial networks, mitophagy, contact with endoplasmic reticulum and other organelles, regulation under physiological and pathological conditions.
- 6. MICROTUBULES: structure and dynamics of cytoplasmic microtubules in different types of eukaryotic cells, MTOCs, structure and dynamics of the mitotic spindle, microtubule-directed movement; primary cillia and kinocillia, microtubule toxins.
- 7. ACTIN FILAMENTS: structure and dynamics of actin filaments in different types of eukaryotic cells, focal adhesions and adhesion belts, actin filament-directed movement, contractile ring, muscle contraction, actin filament toxins.
- 8. INTERMEDIATE FILAMENTS: structure and dynamics of intermediate filaments, interactions with other cytoskeletal components, hemidesmosomes and desmosomes, classification of the IF proteins and their expression patterns, practical applications.
- 9. SPATIAL DYNAMICS OF THE PLASMA MEMBRANE: cortical skeleton, spatial organization and dynamics of the cell surface, cell junctions (gap junctions, tight junctions), exocytosis and endocytosis, plasma membrane turnover, extracellular vesicles.
- 10. EXTRACELLULAR MATRIX: glycocalyx of animal cells, ECM components, ECM dynamics and degradation.
- Literature
- Boal D: Mechanics of the Cell, Cambridge University Press 2012, ISBN 978-0521540629
- ALBERTS, Bruce, Alexander JOHNSON, Julian LEWIS, David Owen MORGAN, Martin C. RAFF, Keith ROBERTS and Peter WALTER. Molecular biology of the cell. Edited by John H. Wilson - Tim Hunt. Sixth edition. New York, NY: Garland Science, 2014, xxxiv, 134. ISBN 9780815344322. info
- Teaching methods
- Lectures using computer presentations, discussions with students.
- Assessment methods
- Oral exam, students draw 3 questions from the assigned exam topics.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Listed among pre-requisites of other courses
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2024/Bi7001